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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              18    1135
NP_001035957                                                           18    1135
XP_006721991                                                           18    1135
XP_006721990                                                           18    1135
XP_006721989                                                           18    1135
XP_005258041                                                           18    1135
XP_006721988                                                           18    1135
XP_006721987                                                           18    1135
XP_006721986                                                           18    1135
XP_005258040                                                           18    1135
XP_006721985                                                           18    1135
NP_002903                                                              17    4312
NP_001273361                                                           17    4312
NP_001273360                                                           17    4312
XP_006715607                                                           17    4312
XP_006715606                                                           17    4312
NP_004411                                                              16    5631
NP_057191                                                              16    7355
NP_055989                                                              16    7355
NP_059112                                                              16    7355
XP_006714540                                                           16    7355
NP_775105                                                              15    9605
NP_659436                                                              15    9605
NP_001036148                                                           15    9605
XP_006715506                                                           15    9605
XP_006715505                                                           15    9605
XP_006715504                                                           15    9605
XP_006715503                                                           15    9605
XP_006715502                                                           15    9605

>NP_000258
          Length = 2818

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1414 IERGLKLMS 1422



>NP_001035957
          Length = 2839

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1435 IERGLKLMS 1443



>XP_006721991
          Length = 1632

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1445 IERGLKLMS 1453



>XP_006721990
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1445 IERGLKLMS 1453



>XP_006721989
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1445 IERGLKLMS 1453



>XP_005258041
          Length = 2836

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1414 IERGLKLMS 1422



>XP_006721988
          Length = 2846

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1424 IERGLKLMS 1432



>XP_006721987
          Length = 2849

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1445 IERGLKLMS 1453



>XP_006721986
          Length = 2854

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1432 IERGLKLMS 1440



>XP_005258040
          Length = 2857

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1435 IERGLKLMS 1443



>XP_006721985
          Length = 2867

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IERSLKLMS 9
            IER LKLMS
Sbjct: 1445 IERGLKLMS 1453



>NP_002903
          Length = 3130

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IERSLKLM 8
            +ERSLKL+
Sbjct: 2870 LERSLKLL 2877



>NP_001273361
          Length = 3052

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IERSLKLM 8
            +ERSLKL+
Sbjct: 2792 LERSLKLL 2799



>NP_001273360
          Length = 3052

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IERSLKLM 8
            +ERSLKL+
Sbjct: 2792 LERSLKLL 2799



>XP_006715607
          Length = 3052

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IERSLKLM 8
            +ERSLKL+
Sbjct: 2792 LERSLKLL 2799



>XP_006715606
          Length = 3130

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IERSLKLM 8
            +ERSLKL+
Sbjct: 2870 LERSLKLL 2877



>NP_004411
          Length = 625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ERSLKLMS 9
           ERSLKL++
Sbjct: 297 ERSLKLLA 304



>NP_057191
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ERSLKLMS 9
           ER+LKL+S
Sbjct: 752 ERALKLVS 759



>NP_055989
          Length = 939

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ERSLKLMS 9
           ER+LKL+S
Sbjct: 618 ERALKLVS 625



>NP_059112
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   IERSLKLMS 9
           ++RS+KL+S
Sbjct: 524 LKRSIKLLS 532



>XP_006714540
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ERSLKLMS 9
           ER+LKL+S
Sbjct: 708 ERALKLVS 715



>NP_775105
          Length = 1659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ERSLKLMS 9
           ERS+ LMS
Sbjct: 135 ERSITLMS 142



>NP_659436
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IERSLKLMS 9
           +ER LK+M+
Sbjct: 600 LERQLKVMA 608



>NP_001036148
          Length = 1683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ERSLKLMS 9
           ERS+ LMS
Sbjct: 135 ERSITLMS 142



>XP_006715506
          Length = 1507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ERSLKLMS 9
           ERS+ LMS
Sbjct: 135 ERSITLMS 142



>XP_006715505
          Length = 1673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ERSLKLMS 9
           ERS+ LMS
Sbjct: 135 ERSITLMS 142



>XP_006715504
          Length = 1683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ERSLKLMS 9
           ERS+ LMS
Sbjct: 135 ERSITLMS 142



>XP_006715503
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ERSLKLMS 9
           ERS+ LMS
Sbjct: 135 ERSITLMS 142



>XP_006715502
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ERSLKLMS 9
           ERS+ LMS
Sbjct: 135 ERSITLMS 142



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.130    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 311,036
Number of extensions: 361
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)