Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              19     665
NP_065915                                                              18    1482
XP_005246765                                                           18    1482
NP_056377                                                              16    5631
NP_008949                                                              16    5631
XP_006717007                                                           16    5631
XP_006717006                                                           16    5631
XP_006717005                                                           16    5631
XP_006717004                                                           16    5631
XP_006717003                                                           16    5631
XP_005251736                                                           16    5631
NP_061939                                                              16    7355
NP_115683                                                              16    7355
NP_001099001                                                           16    7355
NP_065870                                                              16    7355
NP_001156001                                                           16    7355
XP_005252328                                                           16    7355
XP_005248808                                                           16    7355
XP_005248807                                                           16    7355
XP_005248806                                                           16    7355
XP_005248805                                                           16    7355
XP_005248804                                                           16    7355
XP_005248803                                                           16    7355
XP_005248802                                                           16    7355
XP_005248801                                                           16    7355
XP_005248800                                                           16    7355
NP_775820                                                              15    9605
XP_006710379                                                           15    9605
XP_006710378                                                           15    9605
>NP_005179
          Length = 906

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IEMVKNILR 9
           IEM KNILR
Sbjct: 885 IEMAKNILR 893
>NP_065915
          Length = 653

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IEMVKNILR 9
           IEMV NILR
Sbjct: 246 IEMVGNILR 254
>XP_005246765
          Length = 653

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IEMVKNILR 9
           IEMV NILR
Sbjct: 246 IEMVGNILR 254
>NP_056377
          Length = 489

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EMVKNILR 9
           E VKNI+R
Sbjct: 191 EAVKNIIR 198
>NP_008949
          Length = 2325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IEMVKNILR 9
            IE+++N+LR
Sbjct: 1043 IELLQNLLR 1051
>XP_006717007
          Length = 1499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IEMVKNILR 9
            IE+++N+LR
Sbjct: 1043 IELLQNLLR 1051
>XP_006717006
          Length = 1760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   IEMVKNILR 9
           IE+++N+LR
Sbjct: 463 IELLQNLLR 471
>XP_006717005
          Length = 1788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   IEMVKNILR 9
           IE+++N+LR
Sbjct: 491 IELLQNLLR 499
>XP_006717004
          Length = 2240

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IEMVKNILR 9
            IE+++N+LR
Sbjct: 1043 IELLQNLLR 1051
>XP_006717003
          Length = 2278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IEMVKNILR 9
            IE+++N+LR
Sbjct: 1043 IELLQNLLR 1051
>XP_005251736
          Length = 2325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IEMVKNILR 9
            IE+++N+LR
Sbjct: 1043 IELLQNLLR 1051
>NP_061939
          Length = 1249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EMVK ILR
Sbjct: 1045 EMVKVILR 1052
>NP_115683
          Length = 341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IEMVKNILR 9
           ++ V+NILR
Sbjct: 127 LDQVRNILR 135
>NP_001099001
          Length = 1319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1257 EMIHNLLR 1264
>NP_065870
          Length = 1302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1240 EMIHNLLR 1247
>NP_001156001
          Length = 1515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1453 EMIHNLLR 1460
>XP_005252328
          Length = 201

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IEMVKNILR 9
           ++ V+NILR
Sbjct: 127 LDQVRNILR 135
>XP_005248808
          Length = 1121

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1059 EMIHNLLR 1066
>XP_005248807
          Length = 1182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1120 EMIHNLLR 1127
>XP_005248806
          Length = 1317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1255 EMIHNLLR 1262
>XP_005248805
          Length = 1345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1283 EMIHNLLR 1290
>XP_005248804
          Length = 1378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1316 EMIHNLLR 1323
>XP_005248803
          Length = 1455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1393 EMIHNLLR 1400
>XP_005248802
          Length = 1515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1453 EMIHNLLR 1460
>XP_005248801
          Length = 1541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1479 EMIHNLLR 1486
>XP_005248800
          Length = 1541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMVKNILR 9
            EM+ N+LR
Sbjct: 1479 EMIHNLLR 1486
>NP_775820
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   IEMVKNI 7
           +EM++NI
Sbjct: 321 VEMIRNI 327
>XP_006710379
          Length = 1452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    IEMVKN 6
            +EMVKN
Sbjct: 1401 VEMVKN 1406
>XP_006710378
          Length = 1478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    IEMVKN 6
            +EMVKN
Sbjct: 1427 VEMVKN 1432
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.148    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 340,408
Number of extensions: 573
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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