Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
NP_705838                                                              16    5631
NP_612429                                                              16    5631
NP_573420                                                              16    5631
NP_573419                                                              16    5631
NP_573418                                                              16    5631
NP_006042                                                              16    5631
NP_071371                                                              16    5631
NP_060667                                                              16    5631
NP_955458                                                              16    5631
XP_005260506                                                           16    5631
XP_006723884                                                           16    5631
XP_005267356                                                           16    5631
NP_063946                                                              16    7355
NP_001137446                                                           16    7355
NP_859067                                                              16    7355
NP_055778                                                              15    9605
NP_001093592                                                           15    9605
XP_005259577                                                           15    9605
XP_005259576                                                           15    9605

>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IDLGRQSQR 9
           IDLG+QSQR
Sbjct: 342 IDLGQQSQR 350



>NP_705838
          Length = 4069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    DLGRQS 7
            DLGRQS
Sbjct: 1644 DLGRQS 1649



>NP_612429
          Length = 5795

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GRQSQR 9
           GRQSQR
Sbjct: 251 GRQSQR 256



>NP_573420
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DLGRQS 7
           DLGRQS
Sbjct: 377 DLGRQS 382



>NP_573419
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DLGRQS 7
           DLGRQS
Sbjct: 379 DLGRQS 384



>NP_573418
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DLGRQS 7
           DLGRQS
Sbjct: 384 DLGRQS 389



>NP_006042
          Length = 493

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DLGRQS 7
           DLGRQS
Sbjct: 386 DLGRQS 391



>NP_071371
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DLGRQSQR 9
           D GRQS R
Sbjct: 353 DTGRQSSR 360



>NP_060667
          Length = 681

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DLGRQSQR 9
           D GRQS R
Sbjct: 407 DTGRQSSR 414



>NP_955458
          Length = 679

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DLGRQSQR 9
           D GRQS R
Sbjct: 405 DTGRQSSR 412



>XP_005260506
          Length = 523

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DLGRQSQR 9
           D GRQS R
Sbjct: 249 DTGRQSSR 256



>XP_006723884
          Length = 625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DLGRQSQR 9
           D GRQS R
Sbjct: 351 DTGRQSSR 358



>XP_005267356
          Length = 5695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GRQSQR 9
           GRQSQR
Sbjct: 151 GRQSQR 156



>NP_063946
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IDLGRQSQR 9
           ID+G  SQR
Sbjct: 158 IDIGMVSQR 166



>NP_001137446
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IDLGRQSQR 9
           ID+G  SQR
Sbjct: 158 IDIGMVSQR 166



>NP_859067
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IDLGRQSQR 9
           +DLG Q +R
Sbjct: 106 LDLGEQRER 114



>NP_055778
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DLGRQSQ 8
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103



>NP_001093592
          Length = 1309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DLGRQSQ 8
            ++GRQSQ
Sbjct: 1040 NIGRQSQ 1046



>XP_005259577
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DLGRQSQ 8
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103



>XP_005259576
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DLGRQSQ 8
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.139    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,909
Number of extensions: 700
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)