Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005912                                                              18    1935
XP_005248577                                                           18    1935
XP_005248576                                                           18    1935
NP_000248                                                              17    2528
XP_005267754                                                           17    2528
XP_005267753                                                           17    2528
NP_001274675                                                           16    5631
NP_001035865                                                           16    5631
NP_061909                                                              16    5631
NP_001274670                                                           16    5631
NP_001073283                                                           16    7355
NP_694567                                                              16    7355
XP_005253356                                                           16    7355
XP_006719092                                                           16    7355
NP_079023                                                              15    9605
NP_001128715                                                           15    9605
NP_001128717                                                           15    9605
NP_001128716                                                           15    9605
NP_001008657                                                           15    9605
NP_000347                                                              15    9605
NP_001182070                                                           15    9605
NP_055469                                                              15    9605
NP_000704                                                              15    9605
NP_003326                                                              15    9605
XP_005268566                                                           15    9605
XP_005268565                                                           15    9605
XP_005268564                                                           15    9605
XP_005268563                                                           15    9605
XP_005268562                                                           15    9605
XP_005268561                                                           15    9605
XP_005268560                                                           15    9605
XP_005268559                                                           15    9605
XP_005265487                                                           15    9605
XP_006713384                                                           15    9605
>NP_005912
          Length = 1512

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IAVFGAAAR 9
            IA FGAAAR
Sbjct: 1386 IADFGAAAR 1394
>XP_005248577
          Length = 1349

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IAVFGAAAR 9
            IA FGAAAR
Sbjct: 1223 IADFGAAAR 1231
>XP_005248576
          Length = 1375

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IAVFGAAAR 9
            IA FGAAAR
Sbjct: 1249 IADFGAAAR 1257
>NP_000248
          Length = 1935

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  IAVFGAAA 8
          +AVFGAAA
Sbjct: 6  MAVFGAAA 13
>XP_005267754
          Length = 1087

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  IAVFGAAA 8
          +AVFGAAA
Sbjct: 6  MAVFGAAA 13
>XP_005267753
          Length = 1935

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  IAVFGAAA 8
          +AVFGAAA
Sbjct: 6  MAVFGAAA 13
>NP_001274675
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  IAVFGAAA 8
          I+VFGAA+
Sbjct: 40 ISVFGAAS 47
>NP_001035865
          Length = 340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IAVFGAAA 8
           I+VFGAA+
Sbjct: 139 ISVFGAAS 146
>NP_061909
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IAVFGAAA 8
           I+VFGAA+
Sbjct: 169 ISVFGAAS 176
>NP_001274670
          Length = 308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IAVFGAAA 8
           I+VFGAA+
Sbjct: 107 ISVFGAAS 114
>NP_001073283
          Length = 183

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVFGAAAR 9
           +VFG AAR
Sbjct: 106 SVFGPAAR 113
>NP_694567
          Length = 163

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AVFGAAAR 9
          +VFG AAR
Sbjct: 86 SVFGPAAR 93
>XP_005253356
          Length = 170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVFGAAAR 9
           +VFG AAR
Sbjct: 93  SVFGPAAR 100
>XP_006719092
          Length = 183

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVFGAAAR 9
           +VFG AAR
Sbjct: 106 SVFGPAAR 113
>NP_079023
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  IAVFGAAAR 9
          ++ FG AAR
Sbjct: 13 LSAFGGAAR 21
>NP_001128715
          Length = 1488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>NP_001128717
          Length = 1412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>NP_001128716
          Length = 1451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>NP_001008657
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>NP_000347
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>NP_001182070
          Length = 1450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>NP_055469
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IAVFGAAAR 9
           +AV GAAA+
Sbjct: 334 VAVGGAAAK 342
>NP_000704
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  IAVFGAAAR 9
          IA+FGA  +
Sbjct: 6  IAIFGATGQ 14
>NP_003326
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IAVFGA 6
           IAVFGA
Sbjct: 419 IAVFGA 424
>XP_005268566
          Length = 994

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005268565
          Length = 995

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005268564
          Length = 1449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005268563
          Length = 1488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005268562
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005268561
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005268560
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005268559
          Length = 1526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAVFGAAAR 9
           +  FGAAA+
Sbjct: 179 VPAFGAAAK 187
>XP_005265487
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IAVFGA 6
           IAVFGA
Sbjct: 419 IAVFGA 424
>XP_006713384
          Length = 1005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IAVFGA 6
           IAVFGA
Sbjct: 419 IAVFGA 424
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.140    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 313,582
Number of extensions: 195
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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