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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_644805                                                              16    7355
NP_003141                                                              16    7355
NP_998827                                                              16    7355
XP_005257675                                                           16    7355
XP_005257674                                                           16    7355
XP_005257673                                                           16    7355
NP_000466                                                              15    9605
NP_660201                                                              15    9605
XP_005276595                                                           15    9605
XP_005256701                                                           15    9605
XP_005274171                                                           15    9605
XP_006718703                                                           15    9605

>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ASSKQAHA 9
            ASSKQAHA
Sbjct: 1472 ASSKQAHA 1479



>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ASSKQAHA 9
            ASSKQAHA
Sbjct: 1472 ASSKQAHA 1479



>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ASSKQAHA 9
            ASSKQAHA
Sbjct: 1472 ASSKQAHA 1479



>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ASSKQAHA 9
            ASSKQAHA
Sbjct: 1472 ASSKQAHA 1479



>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ASSKQAHA 9
            ASSKQAHA
Sbjct: 1472 ASSKQAHA 1479



>NP_644805
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ASSKQAHA 9
          A+SK++HA
Sbjct: 46 AASKESHA 53



>NP_003141
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ASSKQAHA 9
          A+SK++HA
Sbjct: 46 AASKESHA 53



>NP_998827
          Length = 722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ASSKQAHA 9
          A+SK++HA
Sbjct: 46 AASKESHA 53



>XP_005257675
          Length = 863

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ASSKQAHA 9
           A+SK++HA
Sbjct: 140 AASKESHA 147



>XP_005257674
          Length = 864

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ASSKQAHA 9
           A+SK++HA
Sbjct: 140 AASKESHA 147



>XP_005257673
          Length = 864

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ASSKQAHA 9
           A+SK++HA
Sbjct: 141 AASKESHA 148



>NP_000466
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IASSKQAH 8
           + SSKQ H
Sbjct: 150 LTSSKQTH 157



>NP_660201
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ASSKQAH 8
          ASS+Q+H
Sbjct: 91 ASSRQSH 97



>XP_005276595
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IASSKQAHA 9
           +AS  QAHA
Sbjct: 129 VASLGQAHA 137



>XP_005256701
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ASSKQAHA 9
           ASSK+A+A
Sbjct: 681 ASSKEANA 688



>XP_005274171
          Length = 182

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ASSKQAH 8
          ASS+Q+H
Sbjct: 91 ASSRQSH 97



>XP_006718703
          Length = 227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ASSKQAH 8
          ASS+Q+H
Sbjct: 91 ASSRQSH 97



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.100    0.240 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 381,401
Number of extensions: 321
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)