Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     509
NP_001035957                                                           20     509
XP_006721990                                                           20     509
XP_006721989                                                           20     509
XP_005258041                                                           20     509
XP_006721988                                                           20     509
XP_006721987                                                           20     509
XP_006721986                                                           20     509
XP_005258040                                                           20     509
XP_006721985                                                           20     509
NP_005290                                                              17    4312
NP_060592                                                              16    5631
NP_001268223                                                           16    5631
NP_001268222                                                           16    5631
NP_114106                                                              16    5631
NP_036614                                                              16    5631
XP_006719407                                                           16    5631
XP_005248395                                                           16    5631
XP_005248394                                                           16    5631
XP_005245356                                                           16    5631
XP_006711490                                                           16    5631
XP_006711489                                                           16    5631
NP_001004304                                                           16    5631
NP_001442                                                              16    7355
NP_003418                                                              16    7355
XP_005249427                                                           16    7355
XP_005249426                                                           16    7355
XP_005249421                                                           16    7355
NP_115497                                                              15    9605
NP_201591                                                              15    9605
NP_006756                                                              15    9605
NP_839952                                                              15    9605
NP_001480                                                              15    9605
NP_001265475                                                           15    9605
NP_219480                                                              15    9605
NP_001029344                                                           15    9605
NP_001185524                                                           15    9605
NP_001185523                                                           15    9605
NP_758515                                                              15    9605
NP_006864                                                              15    9605
NP_001185522                                                           15    9605
XP_005257805                                                           15    9605
XP_006722205                                                           15    9605
XP_006722204                                                           15    9605
XP_005251975                                                           15    9605
XP_005251974                                                           15    9605
XP_005273704                                                           15    9605
XP_005273703                                                           15    9605
NP_689814                                                              15    9605
NP_003623                                                              15    9605
>NP_000258
          Length = 2818

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2155 IAFRSSYR 2162
>NP_001035957
          Length = 2839

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2176 IAFRSSYR 2183
>XP_006721990
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2186 IAFRSSYR 2193
>XP_006721989
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2186 IAFRSSYR 2193
>XP_005258041
          Length = 2836

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2155 IAFRSSYR 2162
>XP_006721988
          Length = 2846

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2165 IAFRSSYR 2172
>XP_006721987
          Length = 2849

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2186 IAFRSSYR 2193
>XP_006721986
          Length = 2854

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2173 IAFRSSYR 2180
>XP_005258040
          Length = 2857

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2176 IAFRSSYR 2183
>XP_006721985
          Length = 2867

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IAFRSSYR 8
            IAFRSSYR
Sbjct: 2186 IAFRSSYR 2193
>NP_005290
          Length = 319

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FRSSYR 8
           FRSSYR
Sbjct: 289 FRSSYR 294
>NP_060592
          Length = 645

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           IAF  S+RG
Sbjct: 602 IAFPKSFRG 610
>NP_001268223
          Length = 859

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFRSSY 7
           AFRSSY
Sbjct: 233 AFRSSY 238
>NP_001268222
          Length = 895

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFRSSY 7
           AFRSSY
Sbjct: 269 AFRSSY 274
>NP_114106
          Length = 514

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           I+FR +Y G
Sbjct: 191 ISFRGAYHG 199
>NP_036614
          Length = 895

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFRSSY 7
           AFRSSY
Sbjct: 269 AFRSSY 274
>XP_006719407
          Length = 207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFRSSY 7
           AFRSSY
Sbjct: 109 AFRSSY 114
>XP_005248395
          Length = 449

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           I+FR +Y G
Sbjct: 191 ISFRGAYHG 199
>XP_005248394
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           I+FR +Y G
Sbjct: 190 ISFRGAYHG 198
>XP_005245356
          Length = 570

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           IAF  S+RG
Sbjct: 527 IAFPKSFRG 535
>XP_006711490
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           IAF  S+RG
Sbjct: 551 IAFPKSFRG 559
>XP_006711489
          Length = 609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           IAF  S+RG
Sbjct: 566 IAFPKSFRG 574
>NP_001004304
          Length = 193

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFRSSY 7
           AFRSSY
Sbjct: 109 AFRSSY 114
>NP_001442
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FRSSYRG 9
          FR SY+G
Sbjct: 85 FRGSYQG 91
>NP_003418
          Length = 570

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   AFRSSYRG 9
           AFR  Y+G
Sbjct: 164 AFRCGYKG 171
>XP_005249427
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   AFRSSYRG 9
           AFR  Y+G
Sbjct: 164 AFRCGYKG 171
>XP_005249426
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   AFRSSYRG 9
           AFR  Y+G
Sbjct: 164 AFRCGYKG 171
>XP_005249421
          Length = 570

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   AFRSSYRG 9
           AFR  Y+G
Sbjct: 164 AFRCGYKG 171
>NP_115497
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   FRSSYRG 9
           FRS Y G
Sbjct: 353 FRSKYHG 359
>NP_201591
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   IAFRSSYRG 9
           +AFR  Y G
Sbjct: 208 MAFREQYMG 216
>NP_006756
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   FRSSYRG 9
           FRS Y G
Sbjct: 333 FRSKYHG 339
>NP_839952
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   FRSSYRG 9
           FRS Y G
Sbjct: 333 FRSKYHG 339
>NP_001480
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   IAFRSSYRG 9
           +AFR  Y G
Sbjct: 203 MAFREQYMG 211
>NP_001265475
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   IAFRSSYRG 9
           +AFR  Y G
Sbjct: 204 MAFREQYMG 212
>NP_219480
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FRSSYRG 9
          FR+ YRG
Sbjct: 77 FRNLYRG 83
>NP_001029344
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FRSSYRG 9
          FR+ YRG
Sbjct: 87 FRNLYRG 93
>NP_001185524
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           +A RSSY G
Sbjct: 552 LAQRSSYAG 560
>NP_001185523
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           +A RSSY G
Sbjct: 552 LAQRSSYAG 560
>NP_758515
          Length = 1182

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           +A RSSY G
Sbjct: 552 LAQRSSYAG 560
>NP_006864
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           +A RSSY G
Sbjct: 552 LAQRSSYAG 560
>NP_001185522
          Length = 1158

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IAFRSSYRG 9
           +A RSSY G
Sbjct: 552 LAQRSSYAG 560
>XP_005257805
          Length = 1308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IAFRSSYRG 9
           +A+R ++RG
Sbjct: 238 LAYRHNFRG 246
>XP_006722205
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IAFRSSYRG 9
           +A+R ++RG
Sbjct: 314 LAYRHNFRG 322
>XP_006722204
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IAFRSSYRG 9
           +A+R ++RG
Sbjct: 314 LAYRHNFRG 322
>XP_005251975
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   IAFRSSYRG 9
           +AFR  Y G
Sbjct: 166 MAFREQYMG 174
>XP_005251974
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   IAFRSSYRG 9
           +AFR  Y G
Sbjct: 207 MAFREQYMG 215
>XP_005273704
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   FRSSYRG 9
           FRS Y G
Sbjct: 333 FRSKYHG 339
>XP_005273703
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   FRSSYRG 9
           FRS Y G
Sbjct: 333 FRSKYHG 339
>NP_689814
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFRSSYR 8
           +FRSS+R
Sbjct: 150 SFRSSFR 156
>NP_003623
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IAFRSSYRG 9
           +A+R ++RG
Sbjct: 314 LAYRHNFRG 322
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.141    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 462,286
Number of extensions: 685
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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