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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              18    1135
NP_066124                                                              18    1135
XP_006717999                                                           18    1135
NP_003046                                                              17    4312
XP_005273301                                                           16    7355
NP_003263                                                              16    7355
NP_077013                                                              15    9605
NP_001257822                                                           15    9605
NP_001257821                                                           15    9605
NP_001257820                                                           15    9605

>NP_065681
          Length = 1072

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAATVLFSF 9
           IAA VLFSF
Sbjct: 638 IAAAVLFSF 646



>NP_066124
          Length = 1114

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAATVLFSF 9
           IAA VLFSF
Sbjct: 638 IAAAVLFSF 646



>XP_006717999
          Length = 1207

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAATVLFSF 9
           IAA VLFSF
Sbjct: 731 IAAAVLFSF 739



>NP_003046
          Length = 532

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AATVLFSF 9
           A+TVLF+F
Sbjct: 164 ASTVLFAF 171



>XP_005273301
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  TVLFSF 9
          TVLFSF
Sbjct: 93 TVLFSF 98



>NP_003263
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  TVLFSF 9
          TVLFSF
Sbjct: 93 TVLFSF 98



>NP_077013
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1 IAATVLFSF 9
         +A TVLF F
Sbjct: 1 MADTVLFEF 9



>NP_001257822
          Length = 113

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1 IAATVLFSF 9
         +A TVLF F
Sbjct: 1 MADTVLFEF 9



>NP_001257821
          Length = 127

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1 IAATVLFSF 9
         +A TVLF F
Sbjct: 1 MADTVLFEF 9



>NP_001257820
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1 IAATVLFSF 9
         +A TVLF F
Sbjct: 1 MADTVLFEF 9



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.145    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 276,867
Number of extensions: 295
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)