Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           20     509
NP_000240                                                              20     509
NP_001161089                                                           20     509
XP_005265218                                                           20     509
NP_001257292                                                           16    5631
NP_001257291                                                           16    5631
NP_005044                                                              16    5631
NP_036519                                                              16    5631
XP_005246443                                                           16    5631
XP_005246440                                                           16    5631
XP_006712429                                                           16    5631
NP_006419                                                              16    7355
NP_003698                                                              16    7355
XP_005255098                                                           16    7355
XP_005255097                                                           16    7355
XP_005247898                                                           16    7355
NP_005468                                                              15    9605
NP_002205                                                              15    9605
NP_003310                                                              15    9605
NP_597676                                                              15    9605
NP_596870                                                              15    9605
NP_001243779                                                           15    9605
NP_596869                                                              15    9605
XP_005249809                                                           15    9605
XP_005249808                                                           15    9605
XP_006712787                                                           15    9605
XP_006712786                                                           15    9605
NP_001254479                                                           15    9605
NP_597681                                                              15    9605
>NP_001245200
          Length = 687

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HVTITMKTA 9
           HVTIT KTA
Sbjct: 112 HVTITTKTA 120
>NP_000240
          Length = 756

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HVTITMKTA 9
           HVTIT KTA
Sbjct: 112 HVTITTKTA 120
>NP_001161089
          Length = 658

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  HVTITMKTA 9
          HVTIT KTA
Sbjct: 14 HVTITTKTA 22
>XP_005265218
          Length = 687

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HVTITMKTA 9
           HVTIT KTA
Sbjct: 112 HVTITTKTA 120
>NP_001257292
          Length = 308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2 VTITMKT 8
         VTIT+KT
Sbjct: 3 VTITLKT 9
>NP_001257291
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2 VTITMKT 8
         VTIT+KT
Sbjct: 3 VTITLKT 9
>NP_005044
          Length = 363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2 VTITMKT 8
         VTIT+KT
Sbjct: 3 VTITLKT 9
>NP_036519
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VTITMKTA 9
          VT+TMK A
Sbjct: 76 VTLTMKVA 83
>XP_005246443
          Length = 506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 HVTITMKT 8
         H T+TM+T
Sbjct: 2 HCTLTMET 9
>XP_005246440
          Length = 532

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 HVTITMKT 8
         H T+TM+T
Sbjct: 2 HCTLTMET 9
>XP_006712429
          Length = 532

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 HVTITMKT 8
         H T+TM+T
Sbjct: 2 HCTLTMET 9
>NP_006419
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VTITMKT 8
           VT+TM+T
Sbjct: 125 VTVTMRT 131
>NP_003698
          Length = 456

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HVTITMKTA 9
           HV I +KTA
Sbjct: 156 HVIIGLKTA 164
>XP_005255098
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VTITMKT 8
           VT+TM+T
Sbjct: 125 VTVTMRT 131
>XP_005255097
          Length = 287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VTITMKT 8
           VT+TM+T
Sbjct: 156 VTVTMRT 162
>XP_005247898
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HVTITMKTA 9
           HV I +KTA
Sbjct: 96  HVIIGLKTA 104
>NP_005468
          Length = 1203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HVTITMKTA 9
            HVT+  KT+
Sbjct: 1149 HVTLPRKTS 1157
>NP_002205
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HVTITMK 7
           +VT+TMK
Sbjct: 431 NVTVTMK 437
>NP_003310
          Length = 26926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2837 HITKTMK 2843
>NP_597676
          Length = 27051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2837 HITKTMK 2843
>NP_596870
          Length = 5604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2883 HITKTMK 2889
>NP_001243779
          Length = 34350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2883 HITKTMK 2889
>NP_596869
          Length = 33423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2883 HITKTMK 2889
>XP_005249809
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HVTITMK 7
           +VT+TMK
Sbjct: 296 NVTVTMK 302
>XP_005249808
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HVTITMK 7
           +VT+TMK
Sbjct: 296 NVTVTMK 302
>XP_006712787
          Length = 33978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2884 HITKTMK 2890
>XP_006712786
          Length = 35484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2884 HITKTMK 2890
>NP_001254479
          Length = 35991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2883 HITKTMK 2889
>NP_597681
          Length = 27118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HVTITMK 7
            H+T TMK
Sbjct: 2837 HITKTMK 2843
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.117    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,221
Number of extensions: 646
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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