Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              20     509
XP_006717990                                                           20     509
XP_006717989                                                           20     509
NP_055660                                                              18    1135
XP_006715685                                                           18    1135
NP_115515                                                              17    2528
XP_005259367                                                           17    2528
XP_006723476                                                           17    2528
XP_006723475                                                           17    2528
NP_004215                                                              17    4312
NP_001017921                                                           17    4312
NP_001008739                                                           17    4312
NP_001071153                                                           16    5631
NP_775866                                                              16    7355
XP_005273481                                                           16    7355
NP_001027006                                                           16    7355
NP_001025041                                                           16    7355
NP_001276980                                                           15    9605
NP_001276979                                                           15    9605
NP_001276981                                                           15    9605
NP_037414                                                              15    9605
NP_001041666                                                           15    9605
NP_002494                                                              15    9605
NP_775841                                                              15    9605
NP_055685                                                              15    9605
NP_001274532                                                           15    9605
NP_003434                                                              15    9605
NP_001161693                                                           15    9605
NP_001257589                                                           15    9605
NP_001257590                                                           15    9605
NP_005876                                                              15    9605
NP_001230045                                                           15    9605
XP_006723290                                                           15    9605
XP_006723289                                                           15    9605
XP_005247326                                                           15    9605
XP_006713625                                                           15    9605
XP_005247324                                                           15    9605
XP_006713624                                                           15    9605
XP_005247323                                                           15    9605
XP_005247322                                                           15    9605
XP_005246404                                                           15    9605
XP_005246043                                                           15    9605
NP_001077090                                                           15    9605
>NP_000305
          Length = 403

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HVAAIHFKA 9
           HVAAIH KA
Sbjct: 118 HVAAIHCKA 126
>XP_006717990
          Length = 175

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HVAAIHFKA 9
           HVAAIH KA
Sbjct: 103 HVAAIHCKA 111
>XP_006717989
          Length = 388

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HVAAIHFKA 9
           HVAAIH KA
Sbjct: 103 HVAAIHCKA 111
>NP_055660
          Length = 907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   HVAAIHF 7
           HVAA+HF
Sbjct: 355 HVAALHF 361
>XP_006715685
          Length = 761

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   HVAAIHF 7
           HVAA+HF
Sbjct: 209 HVAALHF 215
>NP_115515
          Length = 1050

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HVAAIHFKA 9
           HVAA+H +A
Sbjct: 749 HVAALHGRA 757
>XP_005259367
          Length = 854

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HVAAIHFKA 9
           HVAA+H +A
Sbjct: 553 HVAALHGRA 561
>XP_006723476
          Length = 979

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HVAAIHFKA 9
           HVAA+H +A
Sbjct: 749 HVAALHGRA 757
>XP_006723475
          Length = 1050

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HVAAIHFKA 9
           HVAA+H +A
Sbjct: 749 HVAALHGRA 757
>NP_004215
          Length = 617

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AAIHFKA 9
           A++HFKA
Sbjct: 442 ASVHFKA 448
>NP_001017921
          Length = 169

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  HVAAIHFKA 9
          H+AA+H +A
Sbjct: 15 HLAAVHRRA 23
>NP_001008739
          Length = 101

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  HVAAIH 6
          H+AAIH
Sbjct: 41 HIAAIH 46
>NP_001071153
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HV+ +HF
Sbjct: 109 HVSTVHF 115
>NP_775866
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HVAAIH 6
           H+AAIH
Sbjct: 311 HMAAIH 316
>XP_005273481
          Length = 726

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  HVAAIHFK 8
          HV ++HF+
Sbjct: 77 HVRSLHFQ 84
>NP_001027006
          Length = 1149

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  HVAAIHFK 8
          HV ++HF+
Sbjct: 77 HVRSLHFQ 84
>NP_001025041
          Length = 793

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HVAAIH 6
           H+AAIH
Sbjct: 675 HLAAIH 680
>NP_001276980
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 445 HVAGIH 450
>NP_001276979
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 445 HVAGIH 450
>NP_001276981
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 445 HVAGIH 450
>NP_037414
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 458 HVAGIH 463
>NP_001041666
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 445 HVAGIH 450
>NP_002494
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 212 HVAVIH 217
>NP_775841
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HVA+ HF
Sbjct: 104 HVASWHF 110
>NP_055685
          Length = 1239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    HVAAIHFK 8
            HV   HFK
Sbjct: 1122 HVTTEHFK 1129
>NP_001274532
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VAAIHFKA 9
          V  +HFKA
Sbjct: 30 VDGVHFKA 37
>NP_003434
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VAAIHFKA 9
          V  +HFKA
Sbjct: 30 VDGVHFKA 37
>NP_001161693
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HVAAIHFKA 9
           HV+  HF+A
Sbjct: 819 HVSKKHFEA 827
>NP_001257589
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HVAAIHFK 8
           HV A HF+
Sbjct: 276 HVQASHFE 283
>NP_001257590
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HVAAIHFK 8
           HV A HF+
Sbjct: 219 HVQASHFE 226
>NP_005876
          Length = 910

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HVAAIHFK 8
           HV A HF+
Sbjct: 324 HVQASHFE 331
>NP_001230045
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 126 HVAVIH 131
>XP_006723290
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 207 HVAVIH 212
>XP_006723289
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HVAAIH 6
           HVA IH
Sbjct: 272 HVAVIH 277
>XP_005247326
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HVA+ HF
Sbjct: 104 HVASWHF 110
>XP_006713625
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HVA+ HF
Sbjct: 104 HVASWHF 110
>XP_005247324
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HVA+ HF
Sbjct: 98  HVASWHF 104
>XP_006713624
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HVA+ HF
Sbjct: 104 HVASWHF 110
>XP_005247323
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HVA+ HF
Sbjct: 104 HVASWHF 110
>XP_005247322
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HVAAIHF 7
           HVA+ HF
Sbjct: 104 HVASWHF 110
>XP_005246404
          Length = 1821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HVAAIHFKA 9
           HV+  HF+A
Sbjct: 820 HVSKKHFEA 828
>XP_005246043
          Length = 747

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VAAIHFKA 9
          V  +HFKA
Sbjct: 30 VDGVHFKA 37
>NP_001077090
          Length = 1239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    HVAAIHFK 8
            HV   HFK
Sbjct: 1122 HVTTEHFK 1129
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.133    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 528,620
Number of extensions: 891
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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