Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              20     509
NP_001091680                                                           20     509
NP_001091679                                                           20     509
XP_006713048                                                           20     509
XP_006713047                                                           20     509
XP_006713046                                                           20     509
XP_005264943                                                           20     509
NP_002221                                                              19     665
NP_068831                                                              19     665
XP_006721941                                                           19     665
XP_006721940                                                           19     665
XP_006721939                                                           19     665
XP_006721938                                                           19     665
XP_006721937                                                           19     665
XP_006721936                                                           19     665
XP_006721935                                                           19     665
XP_006721934                                                           19     665
XP_005257370                                                           19     665
NP_055690                                                              17    2528
NP_112181                                                              17    4312
NP_001273279                                                           16    5631
NP_001243793                                                           16    5631
NP_001243794                                                           16    5631
NP_055602                                                              16    5631
NP_999627                                                              16    5631
NP_001191304                                                           16    5631
NP_000899                                                              16    5631
NP_001191305                                                           16    5631
NP_004776                                                              16    5631
NP_001123484                                                           16    5631
XP_005248366                                                           16    5631
NP_075054                                                              16    5631
NP_003873                                                              16    7355
NP_543028                                                              16    7355
NP_001275918                                                           16    7355
NP_057425                                                              16    7355
NP_006827                                                              16    7355
NP_071896                                                              16    7355
XP_006717952                                                           16    7355
XP_006717951                                                           16    7355
XP_006717950                                                           16    7355
XP_006717949                                                           16    7355
XP_006717948                                                           16    7355
XP_006716743                                                           16    7355
NP_001004450                                                           16    7355
NP_002448                                                              15    9605
NP_001107039                                                           15    9605
NP_997270                                                              15    9605
NP_000695                                                              15    9605
>NP_001895
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TVLRAGDR 9
           TVLRAGDR
Sbjct: 450 TVLRAGDR 457
>NP_001091680
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TVLRAGDR 9
           TVLRAGDR
Sbjct: 450 TVLRAGDR 457
>NP_001091679
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TVLRAGDR 9
           TVLRAGDR
Sbjct: 450 TVLRAGDR 457
>XP_006713048
          Length = 720

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TVLRAGDR 9
           TVLRAGDR
Sbjct: 450 TVLRAGDR 457
>XP_006713047
          Length = 774

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TVLRAGDR 9
           TVLRAGDR
Sbjct: 443 TVLRAGDR 450
>XP_006713046
          Length = 774

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TVLRAGDR 9
           TVLRAGDR
Sbjct: 443 TVLRAGDR 450
>XP_005264943
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TVLRAGDR 9
           TVLRAGDR
Sbjct: 450 TVLRAGDR 457
>NP_002221
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>NP_068831
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>XP_006721941
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>XP_006721940
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>XP_006721939
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>XP_006721938
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>XP_006721937
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>XP_006721936
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 440 HAILRAGDK 448
>XP_006721935
          Length = 762

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 457 HAILRAGDK 465
>XP_006721934
          Length = 783

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 478 HAILRAGDK 486
>XP_005257370
          Length = 783

 Score = 19.2 bits (38), Expect =   665
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           H +LRAGD+
Sbjct: 478 HAILRAGDK 486
>NP_055690
          Length = 1648

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TVLRAGDR 9
           TVLR GDR
Sbjct: 880 TVLRHGDR 887
>NP_112181
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HTVLRAGDR 9
           H +LRA +R
Sbjct: 96  HAILRAAER 104
>NP_001273279
          Length = 604

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HTVLRAGDR 9
           HT  R GDR
Sbjct: 519 HTTNRFGDR 527
>NP_001243793
          Length = 970

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HTVLRAGD 8
           HTVL AG+
Sbjct: 898 HTVLWAGE 905
>NP_001243794
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HTVLRAGD 8
           HTVL AG+
Sbjct: 828 HTVLWAGE 835
>NP_055602
          Length = 913

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HTVLRAGD 8
           HTVL AG+
Sbjct: 841 HTVLWAGE 848
>NP_999627
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HTVLRAGDR 9
           HT  R GDR
Sbjct: 689 HTTNRFGDR 697
>NP_001191304
          Length = 541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HTVLRAG 7
           H VLRAG
Sbjct: 373 HEVLRAG 379
>NP_000899
          Length = 540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HTVLRAG 7
           H VLRAG
Sbjct: 373 HEVLRAG 379
>NP_001191305
          Length = 324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HTVLRAG 7
           H VLRAG
Sbjct: 157 HEVLRAG 163
>NP_004776
          Length = 326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LRAGDR 9
          LRAGDR
Sbjct: 50 LRAGDR 55
>NP_001123484
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LRAGDR 9
          LRAGDR
Sbjct: 50 LRAGDR 55
>XP_005248366
          Length = 325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HTVLRAG 7
           H VLRAG
Sbjct: 157 HEVLRAG 163
>NP_075054
          Length = 467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  TVLRAGD 8
          T+LR+GD
Sbjct: 63 TILRSGD 69
>NP_003873
          Length = 367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           HT+++AG +
Sbjct: 264 HTLIKAGKK 272
>NP_543028
          Length = 280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           HT+++AG +
Sbjct: 177 HTLIKAGKK 185
>NP_001275918
          Length = 2286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HTVLRAGD 8
          HTV R+G+
Sbjct: 42 HTVRRSGE 49
>NP_057425
          Length = 2302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HTVLRAGD 8
          HTV R+G+
Sbjct: 42 HTVRRSGE 49
>NP_006827
          Length = 2671

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    TVLRAGDR 9
            T LRAG R
Sbjct: 1796 TALRAGQR 1803
>NP_071896
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HTVLRAGD 8
           HT++ +GD
Sbjct: 542 HTLIESGD 549
>XP_006717952
          Length = 2300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HTVLRAGD 8
          HTV R+G+
Sbjct: 42 HTVRRSGE 49
>XP_006717951
          Length = 2315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HTVLRAGD 8
          HTV R+G+
Sbjct: 42 HTVRRSGE 49
>XP_006717950
          Length = 2316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HTVLRAGD 8
          HTV R+G+
Sbjct: 42 HTVRRSGE 49
>XP_006717949
          Length = 2316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HTVLRAGD 8
          HTV R+G+
Sbjct: 42 HTVRRSGE 49
>XP_006717948
          Length = 2316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HTVLRAGD 8
          HTV R+G+
Sbjct: 42 HTVRRSGE 49
>XP_006716743
          Length = 202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   HTVLRAGDR 9
           HT+++AG +
Sbjct: 99  HTLIKAGKK 107
>NP_001004450
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HTVLRAG 7
           HT+LR G
Sbjct: 157 HTMLRVG 163
>NP_002448
          Length = 5179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1    HTVLRAGD 8
            H +L+ GD
Sbjct: 5004 HVILKPGD 5011
>NP_001107039
          Length = 344

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLRAGDR 9
          +LRAGD+
Sbjct: 34 LLRAGDK 40
>NP_997270
          Length = 212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLRAGDR 9
          +LRAGD+
Sbjct: 34 LLRAGDK 40
>NP_000695
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TVLRAGDR 9
           TV+R GD+
Sbjct: 181 TVIRDGDK 188
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.140    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 558,639
Number of extensions: 1245
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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