Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_002449                                                              19     869
NP_001005743                                                           17    3301
NP_003735                                                              17    3301
NP_001005745                                                           17    3301
NP_001005744                                                           17    3301
NP_002007                                                              17    3301
XP_006720359                                                           17    3301
XP_006720358                                                           17    3301
XP_006720357                                                           17    3301
XP_006720356                                                           17    3301
XP_006720355                                                           17    3301
XP_006720354                                                           17    3301
XP_006720353                                                           17    3301
XP_006720352                                                           17    3301
XP_006720351                                                           17    3301
XP_006720350                                                           17    3301
XP_006720349                                                           17    3301
XP_006720348                                                           17    3301
XP_005268203                                                           17    3301
XP_005268202                                                           17    3301
XP_005268201                                                           17    3301
XP_006720347                                                           17    3301
XP_005268200                                                           17    3301
XP_006720346                                                           17    3301
XP_005268199                                                           17    3301
NP_000065                                                              16    5631
NP_997191                                                              16    5631
NP_001152956                                                           16    5631
XP_006720320                                                           16    5631
XP_005268130                                                           16    5631
XP_006711682                                                           16    5631
NP_997545                                                              16    5631
NP_694985                                                              16    7355
NP_006738                                                              16    7355
NP_001010905                                                           16    7355
NP_001191827                                                           16    7355
NP_001191826                                                           16    7355
NP_612470                                                              16    7355
NP_066022                                                              16    7355
NP_078928                                                              16    7355
NP_031398                                                              16    7355
NP_987095                                                              16    7355
XP_006723369                                                           16    7355
XP_006721720                                                           16    7355
XP_005274246                                                           16    7355
XP_006711061                                                           16    7355
XP_006711060                                                           16    7355
XP_005244868                                                           16    7355
XP_003119848                                                           16    7355
NP_001098099                                                           16    7355
NP_060772                                                              16    7355
NP_207646                                                              15    9605
NP_055062                                                              15    9605
NP_006589                                                              15    9605
NP_001157860                                                           15    9605
NP_001157859                                                           15    9605
NP_001157858                                                           15    9605
NP_057424                                                              15    9605
XP_006725069                                                           15    9605
XP_006720345                                                           15    9605
XP_005268192                                                           15    9605
XP_005268191                                                           15    9605
XP_005248288                                                           15    9605
XP_006714485                                                           15    9605
XP_005263418                                                           15    9605
XP_005263417                                                           15    9605
XP_006714484                                                           15    9605
XP_005263416                                                           15    9605
XP_006714483                                                           15    9605
XP_005263415                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H EASSRTT
Sbjct: 2104 HPEASSRTT 2112
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H EASSRTT
Sbjct: 1899 HPEASSRTT 1907
>NP_002449
          Length = 5762

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT A+SRTT
Sbjct: 2832 HTRATSRTT 2840

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT A+SRTT
Sbjct: 2275 HTTATSRTT 2283

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT A+SRTT
Sbjct: 4089 HTTATSRTT 4097

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    HTEASSRTT 9
            HT  +SRTT
Sbjct: 3532 HTRGTSRTT 3540
>NP_001005743
          Length = 651

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 564 HYEASSATT 572
>NP_003735
          Length = 640

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 553 HYEASSATT 561
>NP_001005745
          Length = 592

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 505 HYEASSATT 513
>NP_001005744
          Length = 603

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 516 HYEASSATT 524
>NP_002007
          Length = 4061

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    HTEASSR 7
            HTE+SSR
Sbjct: 1981 HTESSSR 1987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    HTEASSR 7
            H EASSR
Sbjct: 1885 HHEASSR 1891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    HTEASSR 7
            H EASSR
Sbjct: 2534 HHEASSR 2540
>XP_006720359
          Length = 445

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 358 HYEASSATT 366
>XP_006720358
          Length = 445

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 358 HYEASSATT 366
>XP_006720357
          Length = 456

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 369 HYEASSATT 377
>XP_006720356
          Length = 456

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 369 HYEASSATT 377
>XP_006720355
          Length = 494

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 407 HYEASSATT 415
>XP_006720354
          Length = 494

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 407 HYEASSATT 415
>XP_006720353
          Length = 505

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 418 HYEASSATT 426
>XP_006720352
          Length = 505

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 418 HYEASSATT 426
>XP_006720351
          Length = 553

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 466 HYEASSATT 474
>XP_006720350
          Length = 592

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 505 HYEASSATT 513
>XP_006720349
          Length = 600

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 513 HYEASSATT 521
>XP_006720348
          Length = 603

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 516 HYEASSATT 524
>XP_005268203
          Length = 603

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 516 HYEASSATT 524
>XP_005268202
          Length = 637

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 550 HYEASSATT 558
>XP_005268201
          Length = 640

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 553 HYEASSATT 561
>XP_006720347
          Length = 648

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 561 HYEASSATT 569
>XP_005268200
          Length = 651

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 564 HYEASSATT 572
>XP_006720346
          Length = 651

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 564 HYEASSATT 572
>XP_005268199
          Length = 651

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EASS TT
Sbjct: 564 HYEASSATT 572
>NP_000065
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TEASSRTT 9
           +EASS+TT
Sbjct: 128 SEASSKTT 135
>NP_997191
          Length = 1888

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT+A+ RT+
Sbjct: 1248 HTKAAGRTS 1256
>NP_001152956
          Length = 1736

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT+A+ RT+
Sbjct: 1248 HTKAAGRTS 1256
>XP_006720320
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEASSRTT 9
           TE+ SRTT
Sbjct: 108 TESQSRTT 115
>XP_005268130
          Length = 438

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEASSRTT 9
           TE+ SRTT
Sbjct: 108 TESQSRTT 115
>XP_006711682
          Length = 1739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT+A+ RT+
Sbjct: 1099 HTKAAGRTS 1107
>NP_997545
          Length = 306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEASSRTT 9
           TE+ SRTT
Sbjct: 108 TESQSRTT 115
>NP_694985
          Length = 1042

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HTEASSRT 8
           H+EASS T
Sbjct: 138 HSEASSGT 145
>NP_006738
          Length = 1042

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HTEASSRT 8
           H+EASS T
Sbjct: 138 HSEASSGT 145
>NP_001010905
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   HTEASSRT 8
           H+E+SSR+
Sbjct: 318 HSESSSRS 325
>NP_001191827
          Length = 183

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TEASSRT 8
          T+ASSRT
Sbjct: 7  TQASSRT 13
>NP_001191826
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TEASSRT 8
          T+ASSRT
Sbjct: 7  TQASSRT 13
>NP_612470
          Length = 209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TEASSRT 8
          T+ASSRT
Sbjct: 7  TQASSRT 13
>NP_066022
          Length = 2564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HTEASSR 7
           H EASSR
Sbjct: 803 HDEASSR 809
>NP_078928
          Length = 2015

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H EA+ RTT
Sbjct: 668 HGEATIRTT 676
>NP_031398
          Length = 938

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H E+SSR T
Sbjct: 846 HPESSSRHT 854
>NP_987095
          Length = 938

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H E+SSR T
Sbjct: 846 HPESSSRHT 854
>XP_006723369
          Length = 2394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HTEASSR 7
           H EASSR
Sbjct: 803 HDEASSR 809
>XP_006721720
          Length = 938

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HTEASSRTT 9
           H E+SSR T
Sbjct: 846 HPESSSRHT 854
>XP_005274246
          Length = 1042

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HTEASSRT 8
           H+EASS T
Sbjct: 138 HSEASSGT 145
>XP_006711061
          Length = 1553

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    HTEASSR 7
            H EASSR
Sbjct: 1419 HREASSR 1425
>XP_006711060
          Length = 1595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    HTEASSR 7
            H EASSR
Sbjct: 1461 HREASSR 1467
>XP_005244868
          Length = 1639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    HTEASSR 7
            H EASSR
Sbjct: 1505 HREASSR 1511
>XP_003119848
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HTEASSR 7
           H EASSR
Sbjct: 381 HREASSR 387
>NP_001098099
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TEASSRT 8
          TE+SSRT
Sbjct: 64 TESSSRT 70
>NP_060772
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TEASSRT 8
          TE+SSRT
Sbjct: 64 TESSSRT 70
>NP_207646
          Length = 2572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT+++ +TT
Sbjct: 2556 HTDSNPKTT 2564
>NP_055062
          Length = 1499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1145 HSEISSRSS 1153
>NP_006589
          Length = 1083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HTEASSRT 8
           HT A++RT
Sbjct: 961 HTAAAART 968
>NP_001157860
          Length = 1504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1274 HSEISSRSS 1282
>NP_001157859
          Length = 1509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1279 HSEISSRSS 1287
>NP_001157858
          Length = 1609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1274 HSEISSRSS 1282
>NP_057424
          Length = 1601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1266 HSEISSRSS 1274
>XP_006725069
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HTEASSRT 8
           HT A++RT
Sbjct: 961 HTAAAART 968
>XP_006720345
          Length = 1522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT+++ +TT
Sbjct: 1506 HTDSNPKTT 1514
>XP_005268192
          Length = 1602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT+++ +TT
Sbjct: 1586 HTDSNPKTT 1594
>XP_005268191
          Length = 2572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            HT+++ +TT
Sbjct: 2556 HTDSNPKTT 2564
>XP_005248288
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HTEASSRT 8
           HT A++RT
Sbjct: 961 HTAAAART 968
>XP_006714485
          Length = 1585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1231 HSEISSRSS 1239
>XP_005263418
          Length = 1654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1300 HSEISSRSS 1308
>XP_005263417
          Length = 1660

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1306 HSEISSRSS 1314
>XP_006714484
          Length = 1685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1331 HSEISSRSS 1339
>XP_005263416
          Length = 1693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1339 HSEISSRSS 1347
>XP_006714483
          Length = 1695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1341 HSEISSRSS 1349
>XP_005263415
          Length = 1701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    HTEASSRTT 9
            H+E SSR++
Sbjct: 1347 HSEISSRSS 1355
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289   0.0926    0.228 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 349,714
Number of extensions: 494
Number of successful extensions: 77
Number of sequences better than 10000.0: 72
Number of HSP's gapped: 77
Number of HSP's successfully gapped: 77
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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