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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001071062                                                           17    2528
XP_006720367                                                           17    2528
NP_219499                                                              16    5631
XP_005250691                                                           16    5631
NP_075561                                                              16    7355
NP_001243404                                                           16    7355
XP_006714553                                                           16    7355
XP_005248406                                                           16    7355
XP_005248404                                                           16    7355
XP_005248403                                                           16    7355
XP_006714552                                                           16    7355
XP_005248402                                                           16    7355
NP_079066                                                              15    9605
NP_001278268                                                           15    9605
NP_001258733                                                           15    9605
NP_002616                                                              15    9605
XP_006716542                                                           15    9605
XP_006716541                                                           15    9605
XP_006716540                                                           15    9605
XP_006716539                                                           15    9605
XP_006716538                                                           15    9605
XP_005251368                                                           15    9605
XP_006716537                                                           15    9605
XP_006716536                                                           15    9605
XP_005251364                                                           15    9605
XP_006716535                                                           15    9605
XP_006716534                                                           15    9605
XP_006716533                                                           15    9605
XP_006716532                                                           15    9605
XP_005251363                                                           15    9605
XP_005251362                                                           15    9605

>NP_001071062
          Length = 722

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HSKVASRLQ 9
           HS VA RLQ
Sbjct: 317 HSAVAQRLQ 325



>XP_006720367
          Length = 769

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   HSKVASRLQ 9
           HS VA RLQ
Sbjct: 317 HSAVAQRLQ 325



>NP_219499
          Length = 1663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HSKVASRLQ 9
           H K+A+RL+
Sbjct: 153 HKKLAARLE 161



>XP_005250691
          Length = 1645

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HSKVASRLQ 9
           H K+A+RL+
Sbjct: 135 HKKLAARLE 143



>NP_075561
          Length = 3197

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HSKVAS 6
           HSKVAS
Sbjct: 984 HSKVAS 989



>NP_001243404
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HSKVASRLQ 9
           HSK + R+Q
Sbjct: 412 HSKASPRIQ 420



>XP_006714553
          Length = 2792

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HSKVAS 6
           HSKVAS
Sbjct: 984 HSKVAS 989



>XP_005248406
          Length = 3214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HSKVAS 6
           HSKVAS
Sbjct: 984 HSKVAS 989



>XP_005248404
          Length = 3250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HSKVAS 6
           HSKVAS
Sbjct: 984 HSKVAS 989



>XP_005248403
          Length = 3250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HSKVAS 6
           HSKVAS
Sbjct: 984 HSKVAS 989



>XP_006714552
          Length = 3251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HSKVAS 6
           HSKVAS
Sbjct: 984 HSKVAS 989



>XP_005248402
          Length = 3251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HSKVAS 6
           HSKVAS
Sbjct: 984 HSKVAS 989



>NP_079066
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 855 NKIASKLQ 862



>NP_001278268
          Length = 876

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 510 NKIASKLQ 517



>NP_001258733
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  HSKVASRLQ 9
          HS  +SRLQ
Sbjct: 20 HSSGSSRLQ 28



>NP_002616
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  HSKVASRLQ 9
          HS  +SRLQ
Sbjct: 20 HSSGSSRLQ 28



>XP_006716542
          Length = 1029

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 890 NKIASKLQ 897



>XP_006716541
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 890 NKIASKLQ 897



>XP_006716540
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 713 NKIASKLQ 720



>XP_006716539
          Length = 1152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 890 NKIASKLQ 897



>XP_006716538
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 804 NKIASKLQ 811



>XP_005251368
          Length = 1185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 819 NKIASKLQ 826



>XP_006716537
          Length = 1205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 839 NKIASKLQ 846



>XP_006716536
          Length = 1212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 846 NKIASKLQ 853



>XP_005251364
          Length = 1212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 846 NKIASKLQ 853



>XP_006716535
          Length = 1220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 854 NKIASKLQ 861



>XP_006716534
          Length = 1220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 854 NKIASKLQ 861



>XP_006716533
          Length = 1220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 854 NKIASKLQ 861



>XP_006716532
          Length = 1223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 857 NKIASKLQ 864



>XP_005251363
          Length = 1248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 882 NKIASKLQ 889



>XP_005251362
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKVASRLQ 9
           +K+AS+LQ
Sbjct: 890 NKIASKLQ 897



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.114    0.287 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,911
Number of extensions: 439
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)