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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_116321                                                              18    1935
XP_005258102                                                           18    1935
NP_997304                                                              17    3301
NP_066022                                                              17    4312
XP_006723369                                                           17    4312
NP_056127                                                              16    7355
XP_006714407                                                           16    7355
XP_006714405                                                           16    7355
XP_005263349                                                           16    7355
XP_005263348                                                           16    7355
XP_005263347                                                           16    7355
XP_005263346                                                           16    7355
XP_005263345                                                           16    7355
XP_005263344                                                           16    7355
XP_005263343                                                           16    7355
XP_005263342                                                           16    7355
XP_005263341                                                           16    7355
XP_005263340                                                           16    7355
XP_005263339                                                           16    7355
NP_004971                                                              15    9605
NP_751948                                                              15    9605
NP_036377                                                              15    9605
NP_001030025                                                           15    9605
NP_000319                                                              15    9605
NP_078966                                                              15    9605
NP_060681                                                              15    9605
XP_006724971                                                           15    9605
XP_006724970                                                           15    9605
XP_005272690                                                           15    9605
XP_006724601                                                           15    9605
XP_005267471                                                           15    9605
XP_005267470                                                           15    9605
XP_006710801                                                           15    9605
XP_006710694                                                           15    9605
XP_006710693                                                           15    9605
XP_006710692                                                           15    9605
XP_005270908                                                           15    9605

>NP_116321
          Length = 637

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   HRTTSLISS 9
           H TTSLIS+
Sbjct: 204 HSTTSLISN 212



>XP_005258102
          Length = 607

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   HRTTSLISS 9
           H TTSLIS+
Sbjct: 174 HSTTSLISN 182



>NP_997304
          Length = 694

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   HRTTSLI 7
           H+TTSLI
Sbjct: 370 HKTTSLI 376



>NP_066022
          Length = 2564

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HRTTSLISS 9
           HRT  LIS+
Sbjct: 326 HRTVGLISN 334



>XP_006723369
          Length = 2394

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HRTTSLISS 9
           HRT  LIS+
Sbjct: 326 HRTVGLISN 334



>NP_056127
          Length = 5005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4025 TTSLVSS 4031



>XP_006714407
          Length = 5004

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4024 TTSLVSS 4030



>XP_006714405
          Length = 4103

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 3123 TTSLVSS 3129



>XP_005263349
          Length = 4410

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 3430 TTSLVSS 3436



>XP_005263348
          Length = 4443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 3463 TTSLVSS 3469



>XP_005263347
          Length = 4640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4046 TTSLVSS 4052



>XP_005263346
          Length = 4983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4003 TTSLVSS 4009



>XP_005263345
          Length = 4991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4011 TTSLVSS 4017



>XP_005263344
          Length = 5005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4025 TTSLVSS 4031



>XP_005263343
          Length = 5009

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4029 TTSLVSS 4035



>XP_005263342
          Length = 5025

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4045 TTSLVSS 4051



>XP_005263341
          Length = 5026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4046 TTSLVSS 4052



>XP_005263340
          Length = 5026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4046 TTSLVSS 4052



>XP_005263339
          Length = 5026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    TTSLISS 9
            TTSL+SS
Sbjct: 4046 TTSLVSS 4052



>NP_004971
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RTTSLISS 9
           +TTSLI S
Sbjct: 467 KTTSLIES 474



>NP_751948
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RTTSLISS 9
           +TTSLI S
Sbjct: 467 KTTSLIES 474



>NP_036377
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RTTSLISS 9
          R TSL+SS
Sbjct: 30 RQTSLVSS 37



>NP_001030025
          Length = 1152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HRTTSLIS 8
           HRT  L+S
Sbjct: 232 HRTPQLVS 239



>NP_000319
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HRTTSLIS 8
           HRT  L+S
Sbjct: 232 HRTPQLVS 239



>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    HRTTSLIS 8
            H T+SL+S
Sbjct: 6243 HSTSSLVS 6250



>NP_060681
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HRTTSLI 7
           H+T+SLI
Sbjct: 456 HKTSSLI 462



>XP_006724971
          Length = 138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HRTTSL 6
           H+TTSL
Sbjct: 119 HKTTSL 124



>XP_006724970
          Length = 138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HRTTSL 6
           H+TTSL
Sbjct: 119 HKTTSL 124



>XP_005272690
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HRTTSLIS 8
           HRT  L+S
Sbjct: 232 HRTPQLVS 239



>XP_006724601
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HRTTSLIS 8
           HRT  L+S
Sbjct: 232 HRTPQLVS 239



>XP_005267471
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RTTSLISS 9
          R TSL+SS
Sbjct: 30 RQTSLVSS 37



>XP_005267470
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RTTSLISS 9
          R TSL+SS
Sbjct: 30 RQTSLVSS 37



>XP_006710801
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HRTTSLI 7
           H+T+SLI
Sbjct: 425 HKTSSLI 431



>XP_006710694
          Length = 510

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RTTSLISS 9
           +TTSLI S
Sbjct: 467 KTTSLIES 474



>XP_006710693
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RTTSLISS 9
           +TTSLI S
Sbjct: 467 KTTSLIES 474



>XP_006710692
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RTTSLISS 9
           +TTSLI S
Sbjct: 467 KTTSLIES 474



>XP_005270908
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RTTSLISS 9
           +TTSLI S
Sbjct: 467 KTTSLIES 474



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.111    0.278 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 359,922
Number of extensions: 517
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)