Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           19     869
NP_036462                                                              19     869
NP_001243397                                                           19     869
XP_005269721                                                           19     869
NP_001273767                                                           17    2528
NP_001501                                                              17    2528
XP_006714258                                                           17    2528
NP_659440                                                              17    3301
NP_776297                                                              17    3301
NP_001230301                                                           17    3301
NP_001820                                                              17    3301
NP_001230303                                                           17    3301
XP_005250885                                                           17    3301
XP_005262783                                                           17    3301
NP_001029097                                                           17    4312
XP_006718022                                                           17    4312
XP_005270165                                                           17    4312
NP_065769                                                              16    5631
XP_006721134                                                           16    5631
XP_005255507                                                           16    5631
NP_060021                                                              16    7355
NP_005236                                                              16    7355
NP_001034864                                                           16    7355
XP_006714202                                                           16    7355
XP_005262892                                                           16    7355
XP_005262891                                                           16    7355
XP_005245975                                                           16    7355
NP_060791                                                              16    7355
NP_829884                                                              15    9605
NP_829883                                                              15    9605
NP_006062                                                              15    9605
>NP_001243398
          Length = 1781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   RRINSSVT 9
           RRINSSVT
Sbjct: 846 RRINSSVT 853
>NP_036462
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RRINSSVT 9
            RRINSSVT
Sbjct: 1138 RRINSSVT 1145
>NP_001243397
          Length = 1890

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   RRINSSVT 9
           RRINSSVT
Sbjct: 955 RRINSSVT 962
>XP_005269721
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RRINSSVT 9
            RRINSSVT
Sbjct: 1138 RRINSSVT 1145
>NP_001273767
          Length = 912

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HRRINSSVT 9
           HRR+NS  T
Sbjct: 783 HRRVNSLCT 791
>NP_001501
          Length = 1007

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HRRINSSVT 9
           HRR+NS  T
Sbjct: 878 HRRVNSLCT 886
>XP_006714258
          Length = 994

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HRRINSSVT 9
           HRR+NS  T
Sbjct: 865 HRRVNSLCT 873
>NP_659440
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RINSSVT 9
           RINSSVT
Sbjct: 270 RINSSVT 276
>NP_776297
          Length = 866

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HRRINS 6
           HRRINS
Sbjct: 104 HRRINS 109
>NP_001230301
          Length = 791

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HRRINS 6
           HRRINS
Sbjct: 104 HRRINS 109
>NP_001820
          Length = 818

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   HRRINS 6
           HRRINS
Sbjct: 104 HRRINS 109
>NP_001230303
          Length = 791

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  HRRINS 6
          HRRINS
Sbjct: 77 HRRINS 82
>XP_005250885
          Length = 878

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RINSSVT 9
           RINSSVT
Sbjct: 337 RINSSVT 343
>XP_005262783
          Length = 839

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  HRRINS 6
          HRRINS
Sbjct: 77 HRRINS 82
>NP_001029097
          Length = 392

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RRINSSV 8
          RR+NSSV
Sbjct: 48 RRVNSSV 54
>XP_006718022
          Length = 309

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RRINSSV 8
          RR+NSSV
Sbjct: 48 RRVNSSV 54
>XP_005270165
          Length = 353

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RRINSSV 8
          RR+NSSV
Sbjct: 9  RRVNSSV 15
>NP_065769
          Length = 1352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RRINSSV 8
           RR+NSSV
Sbjct: 956 RRLNSSV 962
>XP_006721134
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RRINSSV 8
           RR+NSSV
Sbjct: 693 RRLNSSV 699
>XP_005255507
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RRINSSV 8
           RR+NSSV
Sbjct: 693 RRLNSSV 699
>NP_060021
          Length = 1009

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HRRINS 6
           HRR+NS
Sbjct: 877 HRRMNS 882
>NP_005236
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RINSSVT 9
            +INSSVT
Sbjct: 3714 KINSSVT 3720
>NP_001034864
          Length = 1661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RRINSSVT 9
           RR++SSVT
Sbjct: 190 RRMSSSVT 197
>XP_006714202
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RINSSVT 9
            +INSSVT
Sbjct: 3714 KINSSVT 3720
>XP_005262892
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RINSSVT 9
            +INSSVT
Sbjct: 3714 KINSSVT 3720
>XP_005262891
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RINSSVT 9
            +INSSVT
Sbjct: 3714 KINSSVT 3720
>XP_005245975
          Length = 1661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RRINSSVT 9
           RR++SSVT
Sbjct: 190 RRMSSSVT 197
>NP_060791
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HRRINSSVT 9
           HRR+  S+T
Sbjct: 265 HRRVIRSIT 273
>NP_829884
          Length = 1116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  HRRINSS 7
          HRR NS+
Sbjct: 32 HRRTNST 38
>NP_829883
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  HRRINSS 7
          HRR NS+
Sbjct: 32 HRRTNST 38
>NP_006062
          Length = 2253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HRRINSSVT 9
           HR + SSV+
Sbjct: 201 HRAVESSVS 209
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.121    0.327 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 417,315
Number of extensions: 568
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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