Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              21     175
NP_055144                                                              17    3301
NP_000220                                                              17    4312
NP_848537                                                              16    5631
NP_113659                                                              16    5631
NP_001037835                                                           16    5631
NP_942581                                                              16    5631
NP_060200                                                              16    5631
XP_006718768                                                           16    5631
XP_006718767                                                           16    5631
XP_006718766                                                           16    5631
XP_006713275                                                           16    5631
XP_005265305                                                           16    5631
NP_940967                                                              16    7355
NP_689931                                                              15    9605
NP_653302                                                              15    9605
NP_085130                                                              15    9605
NP_005556                                                              15    9605
NP_116216                                                              15    9605
NP_473357                                                              15    9605
NP_001177934                                                           15    9605
NP_536354                                                              15    9605
NP_001172014                                                           15    9605
NP_001667                                                              15    9605
XP_006718526                                                           15    9605
XP_005273893                                                           15    9605
XP_006718525                                                           15    9605
XP_006718524                                                           15    9605
XP_006718523                                                           15    9605
XP_005266053                                                           15    9605
XP_005266052                                                           15    9605
XP_005266051                                                           15    9605
XP_006712185                                                           15    9605
XP_005264680                                                           15    9605
XP_006712184                                                           15    9605
XP_005270574                                                           15    9605
XP_005270573                                                           15    9605
XP_005270569                                                           15    9605
XP_006710363                                                           15    9605
XP_006710361                                                           15    9605
>NP_004295
          Length = 1620

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    HMNMAFSQS 9
            H+NMAFSQS
Sbjct: 1475 HVNMAFSQS 1483
>NP_055144
          Length = 1401

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HMNMAFSQS 9
            H+N AF Q+
Sbjct: 1276 HLNQAFQQA 1284
>NP_000220
          Length = 440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HMNMAFS 7
           H+NM FS
Sbjct: 401 HLNMVFS 407
>NP_848537
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HMNMAFS 7
           H+N+AFS
Sbjct: 616 HINVAFS 622
>NP_113659
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HMNMAFS 7
           H+N+AFS
Sbjct: 612 HINVAFS 618
>NP_001037835
          Length = 326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  MNMAFSQS 9
          + MAFSQS
Sbjct: 23 LGMAFSQS 30
>NP_942581
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>NP_060200
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>XP_006718768
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HMNMAFS 7
           H+N+AFS
Sbjct: 432 HINVAFS 438
>XP_006718767
          Length = 616

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HMNMAFS 7
           H+N+AFS
Sbjct: 565 HINVAFS 571
>XP_006718766
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HMNMAFS 7
           H+N+AFS
Sbjct: 612 HINVAFS 618
>XP_006713275
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>XP_005265305
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>NP_940967
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HMNMAFS 7
           H+N+AF+
Sbjct: 209 HLNLAFN 215
>NP_689931
          Length = 955

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMNMAFSQS 9
           H N+AFS++
Sbjct: 758 HGNVAFSKA 766
>NP_653302
          Length = 629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HMNMAFSQS 9
           H N AFS S
Sbjct: 527 HPNFAFSVS 535
>NP_085130
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HMNMAFSQ 8
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>NP_005556
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HMNMAFSQS 9
           HM  AFS+S
Sbjct: 359 HMPGAFSES 367
>NP_116216
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           H N++FS+
Sbjct: 294 HSNLSFSE 301
>NP_473357
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMNMAFSQS 9
           H N +FS+S
Sbjct: 167 HRNRSFSRS 175
>NP_001177934
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMNMAFSQS 9
           H N +FS+S
Sbjct: 166 HRNRSFSRS 174
>NP_536354
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  HMNMAFS 7
          H+N AFS
Sbjct: 80 HLNPAFS 86
>NP_001172014
          Length = 1045

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   HMNMAFSQS 9
           H N  F QS
Sbjct: 427 HSNQVFDQS 435
>NP_001667
          Length = 1039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   HMNMAFSQS 9
           H N  F QS
Sbjct: 421 HSNQVFDQS 429
>XP_006718526
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMNMAFSQS 9
           H N+AFS++
Sbjct: 475 HGNVAFSKA 483
>XP_005273893
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMNMAFSQS 9
           H N+AFS++
Sbjct: 628 HGNVAFSKA 636
>XP_006718525
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMNMAFSQS 9
           H N+AFS++
Sbjct: 698 HGNVAFSKA 706
>XP_006718524
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMNMAFSQS 9
           H N+AFS++
Sbjct: 715 HGNVAFSKA 723
>XP_006718523
          Length = 947

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMNMAFSQS 9
           H N+AFS++
Sbjct: 750 HGNVAFSKA 758
>XP_005266053
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HMNMAFSQ 8
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>XP_005266052
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HMNMAFSQ 8
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>XP_005266051
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HMNMAFSQ 8
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>XP_006712185
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           H N++FS+
Sbjct: 294 HSNLSFSE 301
>XP_005264680
          Length = 349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           H N++FS+
Sbjct: 294 HSNLSFSE 301
>XP_006712184
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HMNMAFSQ 8
           H N++FS+
Sbjct: 294 HSNLSFSE 301
>XP_005270574
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HMNMAFSQS 9
           H N AFS S
Sbjct: 272 HPNFAFSVS 280
>XP_005270573
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HMNMAFSQS 9
           H N AFS S
Sbjct: 273 HPNFAFSVS 281
>XP_005270569
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HMNMAFSQS 9
           H N AFS S
Sbjct: 324 HPNFAFSVS 332
>XP_006710363
          Length = 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HMNMAFSQS 9
          H N +FS+S
Sbjct: 72 HRNRSFSRS 80
>XP_006710361
          Length = 214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMNMAFSQS 9
           H N +FS+S
Sbjct: 119 HRNRSFSRS 127
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.112    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,700
Number of extensions: 423
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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