Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1135
XP_005271621                                                           18    1135
XP_006718907                                                           18    1135
XP_006718906                                                           18    1135
XP_005271619                                                           18    1135
XP_005271618                                                           18    1135
NP_057006                                                              16    7355
NP_001123237                                                           16    7355
NP_001129735                                                           16    7355
XP_006724887                                                           16    7355
XP_006722712                                                           16    7355
NP_065813                                                              15    9605
NP_536347                                                              15    9605
NP_055665                                                              15    9605
NP_001193942                                                           15    9605
NP_001028289                                                           15    9605
NP_002819                                                              15    9605
NP_536348                                                              15    9605
NP_003095                                                              15    9605
XP_005258184                                                           15    9605
XP_005258183                                                           15    9605
XP_005258182                                                           15    9605
XP_005258181                                                           15    9605
XP_005269099                                                           15    9605
XP_005265679                                                           15    9605
>NP_000042
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MLAAESIN 9
            MLAAESIN
Sbjct: 1087 MLAAESIN 1094
>XP_005271621
          Length = 2708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MLAAESIN 9
           MLAAESIN
Sbjct: 739 MLAAESIN 746
>XP_006718907
          Length = 3050

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MLAAESIN 9
            MLAAESIN
Sbjct: 1081 MLAAESIN 1088
>XP_006718906
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MLAAESIN 9
            MLAAESIN
Sbjct: 1087 MLAAESIN 1094
>XP_005271619
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MLAAESIN 9
            MLAAESIN
Sbjct: 1087 MLAAESIN 1094
>XP_005271618
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MLAAESIN 9
            MLAAESIN
Sbjct: 1087 MLAAESIN 1094
>NP_057006
          Length = 373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMLAAESIN 9
           H+LAAE ++
Sbjct: 247 HILAAEQLS 255
>NP_001123237
          Length = 373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMLAAESIN 9
           H+LAAE ++
Sbjct: 247 HILAAEQLS 255
>NP_001129735
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMLAAESI 8
           H+LAAE +
Sbjct: 325 HLLAAEGL 332
>XP_006724887
          Length = 373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMLAAESIN 9
           H+LAAE ++
Sbjct: 247 HILAAEQLS 255
>XP_006722712
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMLAAESI 8
           H+LAAE +
Sbjct: 325 HLLAAEGL 332
>NP_065813
          Length = 1085

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           HM  AESI+
Sbjct: 169 HMYHAESIS 177
>NP_536347
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 157 HLLQLENIN 165
>NP_055665
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           HM  AESI+
Sbjct: 168 HMYHAESIS 176
>NP_001193942
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 157 HLLQLENIN 165
>NP_001028289
          Length = 1075

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           HM  AESI+
Sbjct: 168 HMYHAESIS 176
>NP_002819
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 157 HLLQLENIN 165
>NP_536348
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 157 HLLQLENIN 165
>NP_003095
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLAAESIN 9
           MLA++S+N
Sbjct: 310 MLASKSVN 317
>XP_005258184
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 128 HLLQLENIN 136
>XP_005258183
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 128 HLLQLENIN 136
>XP_005258182
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 157 HLLQLENIN 165
>XP_005258181
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           H+L  E+IN
Sbjct: 157 HLLQLENIN 165
>XP_005269099
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           HM  AESI+
Sbjct: 169 HMYHAESIS 177
>XP_005265679
          Length = 858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HMLAAESIN 9
           HM  AESI+
Sbjct: 168 HMYHAESIS 176
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.118    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 281,721
Number of extensions: 284
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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