Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005227                                                              17    2528
NP_001032394                                                           17    4312
NP_001010861                                                           17    4312
NP_001121369                                                           16    5631
NP_056087                                                              16    5631
NP_064585                                                              16    5631
NP_001269715                                                           16    5631
NP_653327                                                              16    5631
NP_001186312                                                           16    5631
NP_001186311                                                           16    5631
NP_001186310                                                           16    5631
NP_001186309                                                           16    5631
XP_005255928                                                           16    5631
XP_005255927                                                           16    5631
XP_005255926                                                           16    5631
XP_005255925                                                           16    5631
XP_005255924                                                           16    5631
XP_005252595                                                           16    5631
XP_005252594                                                           16    5631
XP_006717543                                                           16    5631
XP_006717542                                                           16    5631
XP_005265883                                                           16    5631
NP_002961                                                              16    7355
NP_116288                                                              16    7355
NP_996889                                                              16    7355
NP_063944                                                              16    7355
NP_001119809                                                           16    7355
XP_006711588                                                           16    7355
XP_005245531                                                           16    7355
NP_064512                                                              16    7355
NP_783864                                                              15    9605
NP_000206                                                              15    9605
NP_055260                                                              15    9605
NP_060613                                                              15    9605
XP_005274821                                                           15    9605
XP_005274817                                                           15    9605
XP_006722808                                                           15    9605
XP_005259953                                                           15    9605
XP_005262701                                                           15    9605
NP_001010897                                                           15    9605
>NP_005227
          Length = 916

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   HMKNISQMA 9
           H+KNI+++A
Sbjct: 869 HVKNIAELA 877
>NP_001032394
          Length = 321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           HM N++QM
Sbjct: 285 HMDNLAQM 292
>NP_001010861
          Length = 316

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           HM N++QM
Sbjct: 285 HMDNLAQM 292
>NP_001121369
          Length = 1235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMKNISQMA 9
           HM+++SQ A
Sbjct: 765 HMQSLSQQA 773
>NP_056087
          Length = 1315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMKNISQMA 9
           HM+++SQ A
Sbjct: 765 HMQSLSQQA 773
>NP_064585
          Length = 225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  HMKNISQ 7
          H+KNIS+
Sbjct: 88 HLKNISR 94
>NP_001269715
          Length = 190

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  HMKNISQ 7
          H+KNIS+
Sbjct: 88 HLKNISR 94
>NP_653327
          Length = 691

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+KN SQ+
Sbjct: 605 HLKNSSQL 612
>NP_001186312
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+KN SQ+
Sbjct: 577 HLKNSSQL 584
>NP_001186311
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+KN SQ+
Sbjct: 591 HLKNSSQL 598
>NP_001186310
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+KN SQ+
Sbjct: 594 HLKNSSQL 601
>NP_001186309
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+KN SQ+
Sbjct: 607 HLKNSSQL 614
>XP_005255928
          Length = 684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMKNISQMA 9
           HM+++SQ A
Sbjct: 180 HMQSLSQQA 188
>XP_005255927
          Length = 1195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMKNISQMA 9
           HM+++SQ A
Sbjct: 769 HMQSLSQQA 777
>XP_005255926
          Length = 1269

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMKNISQMA 9
           HM+++SQ A
Sbjct: 765 HMQSLSQQA 773
>XP_005255925
          Length = 1273

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMKNISQMA 9
           HM+++SQ A
Sbjct: 769 HMQSLSQQA 777
>XP_005255924
          Length = 1281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HMKNISQMA 9
           HM+++SQ A
Sbjct: 765 HMQSLSQQA 773
>XP_005252595
          Length = 208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  HMKNISQ 7
          H+KNIS+
Sbjct: 71 HLKNISR 77
>XP_005252594
          Length = 208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  HMKNISQ 7
          H+KNIS+
Sbjct: 71 HLKNISR 77
>XP_006717543
          Length = 212

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  HMKNISQ 7
          H+KNIS+
Sbjct: 75 HLKNISR 81
>XP_006717542
          Length = 213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  HMKNISQ 7
          H+KNIS+
Sbjct: 76 HLKNISR 82
>XP_005265883
          Length = 679

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+KN SQ+
Sbjct: 593 HLKNSSQL 600
>NP_002961
          Length = 171

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MKNISQMA 9
           +KN+SQ+A
Sbjct: 110 LKNLSQVA 117
>NP_116288
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HMKNIS 6
           H+KNIS
Sbjct: 214 HLKNIS 219
>NP_996889
          Length = 367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HMKNIS 6
           H+KNIS
Sbjct: 214 HLKNIS 219
>NP_063944
          Length = 467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H KNIS++
Sbjct: 380 HFKNISRI 387
>NP_001119809
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HMKNISQM 8
          H KNI+Q+
Sbjct: 67 HEKNINQL 74
>XP_006711588
          Length = 279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H KNI+Q+
Sbjct: 111 HEKNINQL 118
>XP_005245531
          Length = 409

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H KNI+Q+
Sbjct: 111 HEKNINQL 118
>NP_064512
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HMKNISQM 8
          H KNI+Q+
Sbjct: 92 HEKNINQL 99
>NP_783864
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   HMKNIS 6
           H+KN+S
Sbjct: 117 HLKNVS 122
>NP_000206
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+K ISQ+
Sbjct: 821 HLKYISQL 828
>NP_055260
          Length = 66

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  HMKNISQ 7
          H KNI+Q
Sbjct: 14 HSKNITQ 20
>NP_060613
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HMKNISQMA 9
           HM  +SQ+A
Sbjct: 213 HMYTLSQLA 221
>XP_005274821
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HMKNISQMA 9
           H+K   QMA
Sbjct: 127 HLKRCQQMA 135
>XP_005274817
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HMKNISQMA 9
           H+K   QMA
Sbjct: 195 HLKRCQQMA 203
>XP_006722808
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+K ISQ+
Sbjct: 821 HLKYISQL 828
>XP_005259953
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HMKNISQM 8
           H+K ISQ+
Sbjct: 864 HLKYISQL 871
>XP_005262701
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   HMKNIS 6
           H+KN+S
Sbjct: 117 HLKNVS 122
>NP_001010897
          Length = 65

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  HMKNISQ 7
          H KNI+Q
Sbjct: 14 HSKNITQ 20
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.115    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,456
Number of extensions: 627
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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