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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              18    1482
NP_002323                                                              17    2528
NP_001269166                                                           17    4312
NP_001269167                                                           17    4312
NP_075049                                                              17    4312
NP_612808                                                              17    4312
NP_001245344                                                           16    7355
NP_065860                                                              16    7355
NP_001245347                                                           16    7355
NP_001245346                                                           16    7355
NP_001245345                                                           16    7355
XP_006718956                                                           16    7355
NP_079023                                                              15    9605
NP_065194                                                              15    9605
NP_653271                                                              15    9605
NP_001269353                                                           15    9605
NP_078896                                                              15    9605
XP_006723447                                                           15    9605
XP_006723446                                                           15    9605
XP_006723445                                                           15    9605
XP_006723444                                                           15    9605

>NP_004110
          Length = 993

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HLSQSALVT 9
           H SQSALVT
Sbjct: 335 HPSQSALVT 343



>NP_002323
          Length = 4544

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    HLSQSAL 7
            HLSQSAL
Sbjct: 1305 HLSQSAL 1311



>NP_001269166
          Length = 893

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  HLSQSALVT 9
          HLSQ  L+T
Sbjct: 11 HLSQRELIT 19



>NP_001269167
          Length = 822

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  HLSQSALVT 9
          HLSQ  L+T
Sbjct: 11 HLSQRELIT 19



>NP_075049
          Length = 823

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  HLSQSALVT 9
          HLSQ  L+T
Sbjct: 11 HLSQRELIT 19



>NP_612808
          Length = 894

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  HLSQSALVT 9
          HLSQ  L+T
Sbjct: 11 HLSQRELIT 19



>NP_001245344
          Length = 1168

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HLSQSALVT 9
           HL  SAL+T
Sbjct: 247 HLRDSALLT 255



>NP_065860
          Length = 1191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HLSQSALVT 9
           HL  SAL+T
Sbjct: 270 HLRDSALLT 278



>NP_001245347
          Length = 1155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HLSQSALVT 9
           HL  SAL+T
Sbjct: 234 HLRDSALLT 242



>NP_001245346
          Length = 1155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HLSQSALVT 9
           HL  SAL+T
Sbjct: 234 HLRDSALLT 242



>NP_001245345
          Length = 1165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HLSQSALVT 9
           HL  SAL+T
Sbjct: 244 HLRDSALLT 252



>XP_006718956
          Length = 1191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HLSQSALVT 9
           HL  SAL+T
Sbjct: 270 HLRDSALLT 278



>NP_079023
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HLSQSALVT 9
           HL  SAL+T
Sbjct: 445 HLPPSALLT 453



>NP_065194
          Length = 1819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    HLSQSAL 7
            H SQSAL
Sbjct: 1300 HTSQSAL 1306



>NP_653271
          Length = 1454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSQSALVT 9
            +SQS LVT
Sbjct: 1419 ISQSVLVT 1426



>NP_001269353
          Length = 963

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSQSALVT 9
           +SQS LVT
Sbjct: 928 ISQSVLVT 935



>NP_078896
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  HLSQSAL 7
          HL QSAL
Sbjct: 46 HLRQSAL 52



>XP_006723447
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  HLSQSAL 7
          HL QSAL
Sbjct: 46 HLRQSAL 52



>XP_006723446
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  HLSQSAL 7
          HL QSAL
Sbjct: 46 HLRQSAL 52



>XP_006723445
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  HLSQSAL 7
          HL QSAL
Sbjct: 46 HLRQSAL 52



>XP_006723444
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  HLSQSAL 7
          HL QSAL
Sbjct: 46 HLRQSAL 52



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.114    0.282 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 287,704
Number of extensions: 248
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)