Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001121619                                                           19     869
NP_000258                                                              19     869
NP_001035957                                                           19     869
XP_006721991                                                           19     869
XP_006721990                                                           19     869
XP_006721989                                                           19     869
XP_005258041                                                           19     869
XP_006721988                                                           19     869
XP_006721987                                                           19     869
XP_006721986                                                           19     869
XP_005258040                                                           19     869
XP_006721985                                                           19     869
NP_689691                                                              17    2528
XP_005258616                                                           17    2528
XP_005258615                                                           17    2528
NP_001153332                                                           17    2528
NP_001153333                                                           17    2528
NP_009149                                                              17    4312
NP_060238                                                              16    5631
XP_005271070                                                           16    5631
XP_006710820                                                           16    5631
NP_060420                                                              16    7355
NP_000112                                                              16    7355
NP_001230271                                                           16    7355
NP_003156                                                              16    7355
NP_078953                                                              16    7355
XP_005257549                                                           16    7355
NP_001027392                                                           16    7355
NP_037514                                                              15    9605
NP_061863                                                              15    9605
NP_065772                                                              15    9605
XP_006724826                                                           15    9605
XP_005271739                                                           15    9605
NP_003469                                                              15    9605
NP_036492                                                              15    9605
>NP_001121619
          Length = 593

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>NP_000258
          Length = 2818

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>NP_001035957
          Length = 2839

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_006721991
          Length = 1632

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_006721990
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_006721989
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_005258041
          Length = 2836

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_006721988
          Length = 2846

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_006721987
          Length = 2849

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_006721986
          Length = 2854

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 67 YLSQLIILD 75
>XP_005258040
          Length = 2857

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>XP_006721985
          Length = 2867

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  HLSQLIILD 9
          +LSQLIILD
Sbjct: 80 YLSQLIILD 88
>NP_689691
          Length = 569

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   HLSQLII 7
           HLSQLII
Sbjct: 138 HLSQLII 144
>XP_005258616
          Length = 526

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   HLSQLII 7
           HLSQLII
Sbjct: 95  HLSQLII 101
>XP_005258615
          Length = 570

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   HLSQLII 7
           HLSQLII
Sbjct: 139 HLSQLII 145
>NP_001153332
          Length = 569

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   HLSQLII 7
           HLSQLII
Sbjct: 138 HLSQLII 144
>NP_001153333
          Length = 569

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   HLSQLII 7
           HLSQLII
Sbjct: 138 HLSQLII 144
>NP_009149
          Length = 664

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   SQLIILD 9
           SQLIILD
Sbjct: 152 SQLIILD 158
>NP_060238
          Length = 602

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   HLSQLIILD 9
           HL+ +++LD
Sbjct: 287 HLNSILVLD 295
>XP_005271070
          Length = 384

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  HLSQLIILD 9
          HL+ +++LD
Sbjct: 69 HLNSILVLD 77
>XP_006710820
          Length = 432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   HLSQLIILD 9
           HL+ +++LD
Sbjct: 117 HLNSILVLD 125
>NP_060420
          Length = 1142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SQLIILD 9
           SQL++LD
Sbjct: 276 SQLVVLD 282
>NP_000112
          Length = 508

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   HLSQLIILD 9
           H++++++LD
Sbjct: 138 HINEVVLLD 146
>NP_001230271
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SQLIILD 9
           SQL++LD
Sbjct: 276 SQLVVLD 282
>NP_003156
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SQLIILD 9
           SQL+ILD
Sbjct: 228 SQLLILD 234
>NP_078953
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HLSQLIIL 8
           HLS LII+
Sbjct: 122 HLSSLIIV 129
>XP_005257549
          Length = 579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SQLIILD 9
           SQL++LD
Sbjct: 276 SQLVVLD 282
>NP_001027392
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SQLIILD 9
           SQL+ILD
Sbjct: 228 SQLLILD 234
>NP_037514
          Length = 750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSQLIILD 9
           LSQ I+LD
Sbjct: 189 LSQYILLD 196
>NP_061863
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HLSQLIILD 9
           HL++L I+D
Sbjct: 291 HLNELNIMD 299
>NP_065772
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HLSQLIIL 8
           H SQL++L
Sbjct: 123 HASQLLLL 130
>XP_006724826
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HLSQLIILD 9
           HL++L I+D
Sbjct: 341 HLNELNIMD 349
>XP_005271739
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HLSQLIILD 9
           HL++ +ILD
Sbjct: 398 HLTRRLILD 406
>NP_003469
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HLSQLIILD 9
           HL++ +ILD
Sbjct: 457 HLTRRLILD 465
>NP_036492
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   HLSQLIIL 8
           HL+++IIL
Sbjct: 193 HLNEVIIL 200
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.152    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 391,074
Number of extensions: 711
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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