Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              21     175
XP_006717989                                                           21     175
NP_115784                                                              16    5631
NP_001139634                                                           16    5631
NP_001167554                                                           16    5631
NP_001167553                                                           16    5631
NP_003972                                                              16    5631
NP_060322                                                              16    5631
NP_955445                                                              16    5631
NP_001167552                                                           16    5631
NP_001258871                                                           16    5631
NP_001254509                                                           16    5631
XP_006720823                                                           16    5631
XP_006720822                                                           16    5631
XP_005255044                                                           16    5631
XP_005255043                                                           16    5631
XP_006716433                                                           16    5631
XP_005273633                                                           16    5631
XP_006716432                                                           16    5631
XP_005273632                                                           16    5631
NP_112561                                                              16    5631
XP_005274573                                                           16    7355
NP_001093326                                                           15    9605
NP_001093327                                                           15    9605
NP_003630                                                              15    9605
NP_065194                                                              15    9605
NP_071761                                                              15    9605
NP_060875                                                              15    9605
XP_006726572                                                           15    9605
XP_006726571                                                           15    9605
XP_006726570                                                           15    9605
XP_003960511                                                           15    9605
XP_005274821                                                           15    9605
XP_005274820                                                           15    9605
XP_005274818                                                           15    9605
XP_006724916                                                           15    9605
XP_005274817                                                           15    9605
XP_005258057                                                           15    9605
XP_006717414                                                           15    9605
XP_006710283                                                           15    9605
XP_006710282                                                           15    9605
NP_919415                                                              15    9605
NP_003565                                                              15    9605
>NP_000305
          Length = 403

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   HKMMFETI 8
           HKMMFETI
Sbjct: 196 HKMMFETI 203
>XP_006717989
          Length = 388

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   HKMMFETI 8
           HKMMFETI
Sbjct: 181 HKMMFETI 188
>NP_115784
          Length = 1483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HKMMFETIQ 9
            H M F+T+Q
Sbjct: 1382 HPMDFQTVQ 1390
>NP_001139634
          Length = 450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   HKMMFETIQ 9
           HK++FE ++
Sbjct: 299 HKLLFEDLR 307
>NP_001167554
          Length = 1039

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMMFETI 8
           KM+FET+
Sbjct: 977 KMLFETL 983
>NP_001167553
          Length = 1040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMMFETI 8
           KM+FET+
Sbjct: 978 KMLFETL 984
>NP_003972
          Length = 620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +FE +Q
Sbjct: 348 HKELFEGVQ 356
>NP_060322
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KMMFETI 8
            KM+FET+
Sbjct: 1000 KMLFETL 1006
>NP_955445
          Length = 606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +FE +Q
Sbjct: 348 HKELFEGVQ 356
>NP_001167552
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KMMFETI 8
            KM+FET+
Sbjct: 1000 KMLFETL 1006
>NP_001258871
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   HKMMFETIQ 9
           HK++FE ++
Sbjct: 222 HKLLFEDLR 230
>NP_001254509
          Length = 525

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +FE +Q
Sbjct: 307 HKELFEGVQ 315
>XP_006720823
          Length = 562

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +FE +Q
Sbjct: 348 HKELFEGVQ 356
>XP_006720822
          Length = 563

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +FE +Q
Sbjct: 348 HKELFEGVQ 356
>XP_005255044
          Length = 566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +FE +Q
Sbjct: 348 HKELFEGVQ 356
>XP_005255043
          Length = 626

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +FE +Q
Sbjct: 348 HKELFEGVQ 356
>XP_006716433
          Length = 3069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KMMFETIQ 9
            +MM ETIQ
Sbjct: 1974 QMMMETIQ 1981
>XP_005273633
          Length = 3069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KMMFETIQ 9
            +MM ETIQ
Sbjct: 1974 QMMMETIQ 1981
>XP_006716432
          Length = 3172

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KMMFETIQ 9
            +MM ETIQ
Sbjct: 2077 QMMMETIQ 2084
>XP_005273632
          Length = 3172

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KMMFETIQ 9
            +MM ETIQ
Sbjct: 2077 QMMMETIQ 2084
>NP_112561
          Length = 2789

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KMMFETIQ 9
            +MM ETIQ
Sbjct: 1694 QMMMETIQ 1701
>XP_005274573
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KMMFETIQ 9
           K+MF T+Q
Sbjct: 350 KLMFRTMQ 357
>NP_001093326
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 359 HKIVMETV 366
>NP_001093327
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 291 HKIVMETV 298
>NP_003630
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 291 HKIVMETV 298
>NP_065194
          Length = 1819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           H M+ ET+Q
Sbjct: 172 HSMIQETLQ 180
>NP_071761
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KMMFETIQ 9
           K +FETI+
Sbjct: 371 KTLFETIE 378
>NP_060875
          Length = 184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KMMFETIQ 9
           KM+FET Q
Sbjct: 171 KMVFETGQ 178
>XP_006726572
          Length = 2066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +F T+Q
Sbjct: 938 HKHVFLTVQ 946
>XP_006726571
          Length = 3681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HKMMFETIQ 9
            HK +F T+Q
Sbjct: 2594 HKHVFLTVQ 2602
>XP_006726570
          Length = 3722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HKMMFETIQ 9
            HK +F T+Q
Sbjct: 2594 HKHVFLTVQ 2602
>XP_003960511
          Length = 3722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HKMMFETIQ 9
            HK +F T+Q
Sbjct: 2594 HKHVFLTVQ 2602
>XP_005274821
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 290 HKIVMETV 297
>XP_005274820
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 291 HKIVMETV 298
>XP_005274818
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 359 HKIVMETV 366
>XP_006724916
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 333 HKIVMETV 340
>XP_005274817
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 358 HKIVMETV 365
>XP_005258057
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KMMFETIQ 9
           KM+FET Q
Sbjct: 159 KMVFETGQ 166
>XP_006717414
          Length = 5100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HKMMFETIQ 9
            HK +F T+Q
Sbjct: 3972 HKHVFLTVQ 3980
>XP_006710283
          Length = 2066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIQ 9
           HK +F T+Q
Sbjct: 938 HKHVFLTVQ 946
>XP_006710282
          Length = 2621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HKMMFETIQ 9
            HK +F T+Q
Sbjct: 1493 HKHVFLTVQ 1501
>NP_919415
          Length = 249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKMMFETI 8
           HK M +TI
Sbjct: 93  HKFMVQTI 100
>NP_003565
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKMMFETI 8
           HK M +TI
Sbjct: 93  HKFMVQTI 100
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.133    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 339,544
Number of extensions: 577
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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