Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              21     175
XP_006717989                                                           21     175
NP_001167554                                                           16    5631
NP_001167553                                                           16    5631
NP_060322                                                              16    5631
NP_001167552                                                           16    5631
NP_001191421                                                           16    7355
NP_751911                                                              16    7355
NP_001213                                                              16    7355
NP_751910                                                              16    7355
NP_751913                                                              16    7355
NP_751909                                                              16    7355
NP_001139634                                                           16    7355
NP_001258871                                                           16    7355
XP_006718063                                                           16    7355
XP_006718062                                                           16    7355
XP_005270262                                                           16    7355
XP_005270260                                                           16    7355
XP_005270259                                                           16    7355
XP_006718061                                                           16    7355
XP_005270258                                                           16    7355
XP_005270257                                                           16    7355
XP_006718060                                                           16    7355
XP_005270256                                                           16    7355
XP_005270255                                                           16    7355
XP_005270254                                                           16    7355
XP_006718059                                                           16    7355
XP_006718058                                                           16    7355
XP_005270252                                                           16    7355
XP_006718056                                                           16    7355
NP_001093326                                                           15    9605
NP_001093327                                                           15    9605
NP_003630                                                              15    9605
XP_005274821                                                           15    9605
XP_005274820                                                           15    9605
XP_005274818                                                           15    9605
XP_006724916                                                           15    9605
XP_005274817                                                           15    9605
NP_001005476                                                           15    9605
NP_003619                                                              15    9605
NP_919415                                                              15    9605
NP_003565                                                              15    9605
>NP_000305
          Length = 403

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   HKMMFETI 8
           HKMMFETI
Sbjct: 196 HKMMFETI 203
>XP_006717989
          Length = 388

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   HKMMFETI 8
           HKMMFETI
Sbjct: 181 HKMMFETI 188
>NP_001167554
          Length = 1039

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMMFETI 8
           KM+FET+
Sbjct: 977 KMLFETL 983
>NP_001167553
          Length = 1040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMMFETI 8
           KM+FET+
Sbjct: 978 KMLFETL 984
>NP_060322
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KMMFETI 8
            KM+FET+
Sbjct: 1000 KMLFETL 1006
>NP_001167552
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KMMFETI 8
            KM+FET+
Sbjct: 1000 KMLFETL 1006
>NP_001191421
          Length = 539

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 456 HKFYFENLL 464
>NP_751911
          Length = 556

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 473 HKFYFENLL 481
>NP_001213
          Length = 495

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 412 HKFYFENLL 420
>NP_751910
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 435 HKFYFENLL 443
>NP_751913
          Length = 504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 421 HKFYFENLL 429
>NP_751909
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 444 HKFYFENLL 452
>NP_001139634
          Length = 450

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++FE +
Sbjct: 299 HKLLFEDL 306
>NP_001258871
          Length = 373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++FE +
Sbjct: 222 HKLLFEDL 229
>XP_006718063
          Length = 337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 254 HKFYFENLL 262
>XP_006718062
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 423 HKFYFENLL 431
>XP_005270262
          Length = 516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 433 HKFYFENLL 441
>XP_005270260
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 446 HKFYFENLL 454
>XP_005270259
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 450 HKFYFENLL 458
>XP_006718061
          Length = 536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 453 HKFYFENLL 461
>XP_005270258
          Length = 542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 459 HKFYFENLL 467
>XP_005270257
          Length = 544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 461 HKFYFENLL 469
>XP_006718060
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 467 HKFYFENLL 475
>XP_005270256
          Length = 554

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 471 HKFYFENLL 479
>XP_005270255
          Length = 563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 480 HKFYFENLL 488
>XP_005270254
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 482 HKFYFENLL 490
>XP_006718059
          Length = 567

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 484 HKFYFENLL 492
>XP_006718058
          Length = 574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 491 HKFYFENLL 499
>XP_005270252
          Length = 577

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 494 HKFYFENLL 502
>XP_006718056
          Length = 588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HKMMFETIL 9
           HK  FE +L
Sbjct: 505 HKFYFENLL 513
>NP_001093326
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 359 HKIVMETV 366
>NP_001093327
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 291 HKIVMETV 298
>NP_003630
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 291 HKIVMETV 298
>XP_005274821
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 290 HKIVMETV 297
>XP_005274820
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 291 HKIVMETV 298
>XP_005274818
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 359 HKIVMETV 366
>XP_006724916
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 333 HKIVMETV 340
>XP_005274817
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HKMMFETI 8
           HK++ ET+
Sbjct: 358 HKIVMETV 365
>NP_001005476
          Length = 1149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIL 9
           HK+ F+T L
Sbjct: 680 HKIKFQTSL 688
>NP_003619
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKMMFETIL 9
           HK+ F+T L
Sbjct: 680 HKIKFQTSL 688
>NP_919415
          Length = 249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKMMFETI 8
           HK M +TI
Sbjct: 93  HKFMVQTI 100
>NP_003565
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKMMFETI 8
           HK M +TI
Sbjct: 93  HKFMVQTI 100
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.142    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 360,917
Number of extensions: 616
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package