Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_065810                                                              16    5631
NP_001128302                                                           16    7355
NP_060121                                                              16    7355
NP_001128303                                                           16    7355
NP_001128304                                                           16    7355
NP_001025482                                                           16    7355
NP_963842                                                              16    7355
NP_001025484                                                           16    7355
NP_060853                                                              16    7355
NP_510880                                                              16    7355
NP_004704                                                              16    7355
NP_976063                                                              16    7355
XP_006720684                                                           16    7355
XP_005268258                                                           16    7355
XP_005268257                                                           16    7355
XP_005268256                                                           16    7355
XP_005267096                                                           16    7355
XP_006715570                                                           16    7355
XP_005263754                                                           16    7355
NP_127493                                                              15    9605
NP_001267729                                                           15    9605
NP_127494                                                              15    9605
NP_001157108                                                           15    9605
NP_001509                                                              15    9605
NP_127492                                                              15    9605
NP_079410                                                              15    9605
NP_055516                                                              15    9605
NP_001180418                                                           15    9605
XP_005256229                                                           15    9605
XP_005256227                                                           15    9605
XP_006716009                                                           15    9605
XP_005250353                                                           15    9605
XP_006716008                                                           15    9605
XP_006716007                                                           15    9605
XP_006716006                                                           15    9605
XP_006716005                                                           15    9605
XP_006716004                                                           15    9605
XP_006716003                                                           15    9605
XP_006716002                                                           15    9605
XP_005250348                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KKESTTRL 9
            KKESTTRL
Sbjct: 2237 KKESTTRL 2244
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KKESTTRL 9
            KKESTTRL
Sbjct: 2229 KKESTTRL 2236
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KKESTTRL 9
            KKESTTRL
Sbjct: 2190 KKESTTRL 2197
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KKESTTRL 9
            KKESTTRL
Sbjct: 2269 KKESTTRL 2276
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KKESTTRL 9
            KKESTTRL
Sbjct: 2282 KKESTTRL 2289
>NP_065810
          Length = 4700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKKESTTRL 9
           H KE TT++
Sbjct: 497 HLKEGTTKI 505
>NP_001128302
          Length = 1196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKKESTTRL 9
           H K+ST R+
Sbjct: 818 HTKDSTLRI 826
>NP_060121
          Length = 1196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKKESTTRL 9
           H K+ST R+
Sbjct: 818 HTKDSTLRI 826
>NP_001128303
          Length = 1196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKKESTTRL 9
           H K+ST R+
Sbjct: 818 HTKDSTLRI 826
>NP_001128304
          Length = 1053

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKKESTTRL 9
           H K+ST R+
Sbjct: 818 HTKDSTLRI 826
>NP_001025482
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKKEST 6
           HKKE+T
Sbjct: 178 HKKEAT 183
>NP_963842
          Length = 532

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKKEST 6
           HKKE+T
Sbjct: 178 HKKEAT 183
>NP_001025484
          Length = 463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKKEST 6
           HKKE+T
Sbjct: 178 HKKEAT 183
>NP_060853
          Length = 1336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKKEST 6
           HKKE+T
Sbjct: 330 HKKEAT 335
>NP_510880
          Length = 2054

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KKESTTRL 9
          K+ES TRL
Sbjct: 53 KRESKTRL 60
>NP_004704
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HKKESTTR 8
            H KE+T R
Sbjct: 1035 HSKEATAR 1042
>NP_976063
          Length = 2039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KKESTTRL 9
          K+ES TRL
Sbjct: 53 KRESKTRL 60
>XP_006720684
          Length = 4713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KKESTTRL 9
           KKE TT++
Sbjct: 511 KKEGTTKI 518
>XP_005268258
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKKESTTR 8
           H KE+T R
Sbjct: 806 HSKEATAR 813
>XP_005268257
          Length = 862

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKKESTTR 8
           H KE+T R
Sbjct: 821 HSKEATAR 828
>XP_005268256
          Length = 1055

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HKKESTTR 8
            H KE+T R
Sbjct: 1014 HSKEATAR 1021
>XP_005267096
          Length = 1168

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKKESTTRL 9
           H K+ST R+
Sbjct: 818 HTKDSTLRI 826
>XP_006715570
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HKKESTTRL 9
           H K+ST R+
Sbjct: 818 HTKDSTLRI 826
>XP_005263754
          Length = 1133

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKKEST 6
           HKKE+T
Sbjct: 330 HKKEAT 335
>NP_127493
          Length = 978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HKKESTTR 8
          HK +STT+
Sbjct: 91 HKMKSTTQ 98
>NP_001267729
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HKKESTTR 8
          HK +STT+
Sbjct: 91 HKMKSTTQ 98
>NP_127494
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HKKESTTR 8
          HK +STT+
Sbjct: 91 HKMKSTTQ 98
>NP_001157108
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HKKESTTR 8
          HK +STT+
Sbjct: 91 HKMKSTTQ 98
>NP_001509
          Length = 957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HKKESTTR 8
          HK +STT+
Sbjct: 91 HKMKSTTQ 98
>NP_127492
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HKKESTTR 8
          HK +STT+
Sbjct: 91 HKMKSTTQ 98
>NP_079410
          Length = 2881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KKESTTRL 9
            K+ESTT +
Sbjct: 2205 KRESTTHM 2212
>NP_055516
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKKESTTR 8
           H K+ST R
Sbjct: 483 HTKDSTNR 490
>NP_001180418
          Length = 1101

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HKKESTTR 8
           H K+ST R
Sbjct: 528 HTKDSTNR 535
>XP_005256229
          Length = 2881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KKESTTRL 9
            K+ESTT +
Sbjct: 2205 KRESTTHM 2212
>XP_005256227
          Length = 2897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KKESTTRL 9
            K+ESTT +
Sbjct: 2205 KRESTTHM 2212
>XP_006716009
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
>XP_005250353
          Length = 969

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
>XP_006716008
          Length = 988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
>XP_006716007
          Length = 989

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
>XP_006716006
          Length = 990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
>XP_006716005
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HKKESTTR 8
          HK +STT+
Sbjct: 91 HKMKSTTQ 98
>XP_006716004
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 96  HKMKSTTQ 103
>XP_006716003
          Length = 1009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
>XP_006716002
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
>XP_005250348
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HKKESTTR 8
           HK +STT+
Sbjct: 103 HKMKSTTQ 110
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.112    0.288 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 571,701
Number of extensions: 879
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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