Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     665
XP_006716142                                                           19     665
XP_005250088                                                           19     665
XP_006716141                                                           19     665
XP_005250085                                                           19     665
XP_006716140                                                           19     665
XP_005250084                                                           19     665
XP_005250083                                                           19     665
XP_005250082                                                           19     665
NP_694551                                                              18    1935
NP_001271268                                                           16    5631
NP_940682                                                              16    5631
NP_001271267                                                           16    5631
NP_001271269                                                           16    5631
NP_001129610                                                           15    9605
NP_001274150                                                           15    9605
NP_001274148                                                           15    9605
NP_001274147                                                           15    9605
NP_036556                                                              15    9605
XP_005273155                                                           15    9605
XP_005273153                                                           15    9605
XP_006711335                                                           15    9605
XP_005273152                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4866 HKIIISSSR 4874
>XP_006716142
          Length = 4927

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4882 HKIIISSSR 4890
>XP_005250088
          Length = 4928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4883 HKIIISSSR 4891
>XP_006716141
          Length = 4959

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4914 HKIIISSSR 4922
>XP_005250085
          Length = 4979

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4934 HKIIISSSR 4942
>XP_006716140
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4937 HKIIISSSR 4945
>XP_005250084
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4937 HKIIISSSR 4945
>XP_005250083
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4937 HKIIISSSR 4945
>XP_005250082
          Length = 4983

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    HKFIISSSR 9
            HK IISSSR
Sbjct: 4938 HKIIISSSR 4946
>NP_694551
          Length = 534

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KFIISSSR 9
           KF++SSSR
Sbjct: 444 KFVVSSSR 451
>NP_001271268
          Length = 1303

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HKFIISSS 8
           H+FIIS S
Sbjct: 297 HQFIISPS 304
>NP_940682
          Length = 1247

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HKFIISSS 8
           H+FIIS S
Sbjct: 297 HQFIISPS 304
>NP_001271267
          Length = 1319

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HKFIISSS 8
           H+FIIS S
Sbjct: 297 HQFIISPS 304
>NP_001271269
          Length = 1302

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HKFIISSS 8
           H+FIIS S
Sbjct: 297 HQFIISPS 304
>NP_001129610
          Length = 1054

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 379 HKYIIS 384
>NP_001274150
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 210 HKYIIS 215
>NP_001274148
          Length = 854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 179 HKYIIS 184
>NP_001274147
          Length = 854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 179 HKYIIS 184
>NP_036556
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 391 HKYIIS 396
>XP_005273155
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  HKFIIS 6
          HK+IIS
Sbjct: 39 HKYIIS 44
>XP_005273153
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 210 HKYIIS 215
>XP_006711335
          Length = 945

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 270 HKYIIS 275
>XP_005273152
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HKFIIS 6
           HK+IIS
Sbjct: 360 HKYIIS 365
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.132    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 370,665
Number of extensions: 315
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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