Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              19     869
NP_001001483                                                           17    4312
NP_055959                                                              17    4312
NP_061901                                                              17    4312
XP_005255915                                                           17    4312
XP_005253053                                                           17    4312
NP_958782                                                              16    5631
XP_006716654                                                           16    5631
XP_005251034                                                           16    5631
XP_005251033                                                           16    5631
NP_694574                                                              16    7355
NP_009186                                                              16    7355
NP_001184145                                                           16    7355
NP_001662                                                              16    7355
XP_005256319                                                           16    7355
XP_006721414                                                           16    7355
XP_006714878                                                           16    7355
NP_001124482                                                           15    9605
NP_004360                                                              15    9605
NP_476508                                                              15    9605
NP_476507                                                              15    9605
NP_001128360                                                           15    9605
NP_001073379                                                           15    9605
NP_056483                                                              15    9605
NP_001139192                                                           15    9605
NP_002962                                                              15    9605
NP_001182399                                                           15    9605
NP_079461                                                              15    9605
NP_001273622                                                           15    9605
NP_001273621                                                           15    9605
NP_001140158                                                           15    9605
XP_006721874                                                           15    9605
XP_006721873                                                           15    9605
XP_005257260                                                           15    9605
XP_005257258                                                           15    9605
XP_006721872                                                           15    9605
XP_006721871                                                           15    9605
XP_006721870                                                           15    9605
XP_006721869                                                           15    9605
XP_006719698                                                           15    9605
XP_005269240                                                           15    9605
XP_006719697                                                           15    9605
XP_006719696                                                           15    9605
XP_006719695                                                           15    9605
XP_005269238                                                           15    9605
XP_006716603                                                           15    9605
XP_005250915                                                           15    9605
XP_005250914                                                           15    9605
XP_005250913                                                           15    9605
XP_005247764                                                           15    9605
XP_006713344                                                           15    9605
XP_006713343                                                           15    9605
XP_005246123                                                           15    9605
XP_005246122                                                           15    9605
>NP_004251
          Length = 1208

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IRQFLSLR 9
            IRQFLSLR
Sbjct: 1143 IRQFLSLR 1150
>NP_001001483
          Length = 492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HIRQFLSLR 9
           H++Q LSLR
Sbjct: 434 HMKQTLSLR 442
>NP_055959
          Length = 949

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HIRQFLS 7
           H+R+FLS
Sbjct: 194 HVRRFLS 200
>NP_061901
          Length = 622

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HIRQFLSLR 9
           H++Q LSLR
Sbjct: 564 HMKQTLSLR 572
>XP_005255915
          Length = 1099

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HIRQFLS 7
           H+R+FLS
Sbjct: 331 HVRRFLS 337
>XP_005253053
          Length = 492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HIRQFLSLR 9
           H++Q LSLR
Sbjct: 434 HMKQTLSLR 442
>NP_958782
          Length = 4684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HIRQFLSL 8
          H+RQ+L L
Sbjct: 81 HLRQYLHL 88
>XP_006716654
          Length = 3562

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HIRQFLSL 8
          H+RQ+L L
Sbjct: 81 HLRQYLHL 88
>XP_005251034
          Length = 4685

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HIRQFLSL 8
          H+RQ+L L
Sbjct: 81 HLRQYLHL 88
>XP_005251033
          Length = 4689

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HIRQFLSL 8
          H+RQ+L L
Sbjct: 81 HLRQYLHL 88
>NP_694574
          Length = 896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HIRQFLSL 8
           HI+QF+ L
Sbjct: 133 HIQQFVKL 140
>NP_009186
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HIRQFLS 7
           H+R+FLS
Sbjct: 114 HMRRFLS 120
>NP_001184145
          Length = 252

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  HIRQFLS 7
          H++QF+S
Sbjct: 87 HVKQFVS 93
>NP_001662
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HIRQFLS 7
           H++QF+S
Sbjct: 126 HVKQFVS 132
>XP_005256319
          Length = 678

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HIRQFLSL 8
           HI+QF+ L
Sbjct: 133 HIQQFVKL 140
>XP_006721414
          Length = 810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HIRQFLSL 8
          HI+QF+ L
Sbjct: 47 HIQQFVKL 54
>XP_006714878
          Length = 381

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   HIRQFLS 7
           H+R+FLS
Sbjct: 168 HMRRFLS 174
>NP_001124482
          Length = 763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IRQFLSL 8
           IR+FLSL
Sbjct: 552 IRRFLSL 558
>NP_004360
          Length = 3177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HIRQFLS 7
            HIR F+S
Sbjct: 1454 HIRDFVS 1460
>NP_476508
          Length = 2971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HIRQFLS 7
            HIR F+S
Sbjct: 1248 HIRDFVS 1254
>NP_476507
          Length = 2570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HIRQFLS 7
           HIR F+S
Sbjct: 847 HIRDFVS 853
>NP_001128360
          Length = 1358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    IRQFLSLR 9
            +RQ LSLR
Sbjct: 1101 VRQCLSLR 1108
>NP_001073379
          Length = 1722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QFLSLR 9
           QFLSLR
Sbjct: 821 QFLSLR 826
>NP_056483
          Length = 1264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HIRQFL 6
           H RQFL
Sbjct: 865 HFRQFL 870
>NP_001139192
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   HIRQFL 6
           H++QFL
Sbjct: 276 HVKQFL 281
>NP_002962
          Length = 763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IRQFLSL 8
           IR+FLSL
Sbjct: 552 IRRFLSL 558
>NP_001182399
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IRQFLSL 8
           IR+FLSL
Sbjct: 552 IRRFLSL 558
>NP_079461
          Length = 1990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 497 HLQSMLSLR 505
>NP_001273622
          Length = 1083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HIRQFL 6
           H RQFL
Sbjct: 684 HFRQFL 689
>NP_001273621
          Length = 1252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HIRQFL 6
           H RQFL
Sbjct: 853 HFRQFL 858
>NP_001140158
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HIRQFLS 7
           H+RQ+L+
Sbjct: 110 HLRQYLN 116
>XP_006721874
          Length = 1399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 571 HLQSMLSLR 579
>XP_006721873
          Length = 1409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 571 HLQSMLSLR 579
>XP_005257260
          Length = 1990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 497 HLQSMLSLR 505
>XP_005257258
          Length = 2000

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 497 HLQSMLSLR 505
>XP_006721872
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 534 HLQSMLSLR 542
>XP_006721871
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 534 HLQSMLSLR 542
>XP_006721870
          Length = 2064

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 571 HLQSMLSLR 579
>XP_006721869
          Length = 2074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HIRQFLSLR 9
           H++  LSLR
Sbjct: 571 HLQSMLSLR 579
>XP_006719698
          Length = 899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QFLSLR 9
           QFLSLR
Sbjct: 821 QFLSLR 826
>XP_005269240
          Length = 908

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QFLSLR 9
           QFLSLR
Sbjct: 821 QFLSLR 826
>XP_006719697
          Length = 1658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QFLSLR 9
           QFLSLR
Sbjct: 821 QFLSLR 826
>XP_006719696
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QFLSLR 9
           QFLSLR
Sbjct: 821 QFLSLR 826
>XP_006719695
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QFLSLR 9
           QFLSLR
Sbjct: 821 QFLSLR 826
>XP_005269238
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QFLSLR 9
           QFLSLR
Sbjct: 821 QFLSLR 826
>XP_006716603
          Length = 1168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HIRQFL 6
           H RQFL
Sbjct: 769 HFRQFL 774
>XP_005250915
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HIRQFL 6
           H RQFL
Sbjct: 865 HFRQFL 870
>XP_005250914
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HIRQFL 6
           H RQFL
Sbjct: 865 HFRQFL 870
>XP_005250913
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HIRQFL 6
           H RQFL
Sbjct: 865 HFRQFL 870
>XP_005247764
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HIRQFLS 7
           H+RQ+L+
Sbjct: 118 HLRQYLN 124
>XP_006713344
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IRQFLSL 8
           IR+FLSL
Sbjct: 552 IRRFLSL 558
>XP_006713343
          Length = 763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IRQFLSL 8
           IR+FLSL
Sbjct: 552 IRRFLSL 558
>XP_005246123
          Length = 2770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HIRQFLS 7
            HIR F+S
Sbjct: 1047 HIRDFVS 1053
>XP_005246122
          Length = 2977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    HIRQFLS 7
            HIR F+S
Sbjct: 1254 HIRDFVS 1260
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.350    0.153    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 392,146
Number of extensions: 595
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)

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