Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_149985                                                              17    4312
NP_659484                                                              17    4312
NP_001092908                                                           17    4312
NP_001092907                                                           17    4312
NP_919306                                                              17    4312
XP_006710422                                                           17    4312
XP_005270519                                                           17    4312
XP_005270518                                                           17    4312
NP_705834                                                              17    4312
NP_689813                                                              16    5631
XP_006715914                                                           16    5631
NP_001124463                                                           16    7355
NP_006380                                                              16    7355
NP_001269939                                                           16    7355
NP_001269938                                                           16    7355
NP_116575                                                              16    7355
NP_057518                                                              16    7355
NP_891550                                                              16    7355
NP_570969                                                              16    7355
NP_006355                                                              16    7355
XP_005267319                                                           16    7355
XP_005271451                                                           16    7355
XP_005271450                                                           16    7355
XP_005271449                                                           16    7355
XP_005271447                                                           16    7355
XP_005265386                                                           16    7355
NP_056471                                                              16    7355
NP_000375                                                              15    9605
NP_009041                                                              15    9605
NP_443128                                                              15    9605
NP_004517                                                              15    9605
NP_689966                                                              15    9605
NP_056068                                                              15    9605
NP_689612                                                              15    9605
NP_055692                                                              15    9605
NP_653191                                                              15    9605
NP_006648                                                              15    9605
NP_996848                                                              15    9605
NP_060899                                                              15    9605
XP_006725524                                                           15    9605
XP_006725523                                                           15    9605
XP_005276828                                                           15    9605
XP_006726279                                                           15    9605
XP_006724024                                                           15    9605
XP_005257947                                                           15    9605
XP_005257946                                                           15    9605
XP_005257945                                                           15    9605
XP_006722277                                                           15    9605
XP_005256630                                                           15    9605
XP_005256629                                                           15    9605
XP_005268084                                                           15    9605
XP_005249005                                                           15    9605
XP_005249004                                                           15    9605
XP_005249003                                                           15    9605
XP_005249000                                                           15    9605
XP_006714722                                                           15    9605
XP_006714721                                                           15    9605
XP_005262797                                                           15    9605
XP_005262794                                                           15    9605
>NP_149985
          Length = 549

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H RI SFL+
Sbjct: 521 HSRIGSFLK 529
>NP_659484
          Length = 379

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R+S FL+
Sbjct: 170 HIRVSMFLK 178
>NP_001092908
          Length = 624

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FRISSFL 8
           FR+SSFL
Sbjct: 354 FRLSSFL 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FRISSFL 8
           FR++SFL
Sbjct: 578 FRLNSFL 584
>NP_001092907
          Length = 624

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FRISSFL 8
           FR+SSFL
Sbjct: 354 FRLSSFL 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FRISSFL 8
           FR++SFL
Sbjct: 578 FRLNSFL 584
>NP_919306
          Length = 623

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FRISSFL 8
           FR+SSFL
Sbjct: 353 FRLSSFL 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FRISSFL 8
           FR++SFL
Sbjct: 577 FRLNSFL 583
>XP_006710422
          Length = 227

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R+S FL+
Sbjct: 180 HIRVSMFLK 188
>XP_005270519
          Length = 295

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R+S FL+
Sbjct: 180 HIRVSMFLK 188
>XP_005270518
          Length = 389

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R+S FL+
Sbjct: 180 HIRVSMFLK 188
>NP_705834
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RISSFLR 9
           R+SSFLR
Sbjct: 525 RVSSFLR 531
>NP_689813
          Length = 562

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FRISSFLR 9
           FR++S+LR
Sbjct: 307 FRLASYLR 314
>XP_006715914
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   HFRISSFL 8
           HF +S+FL
Sbjct: 302 HFSLSNFL 309
>NP_001124463
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HFRISSFLR 9
           HF+ISS L+
Sbjct: 135 HFQISSQLQ 143
>NP_006380
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HFRISSFLR 9
           HF+ISS L+
Sbjct: 135 HFQISSQLQ 143
>NP_001269939
          Length = 996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RISSFLR 9
           +ISSFLR
Sbjct: 957 KISSFLR 963
>NP_001269938
          Length = 1300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RISSFLR 9
            +ISSFLR
Sbjct: 1180 KISSFLR 1186
>NP_116575
          Length = 1243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RISSFLR 9
            +ISSFLR
Sbjct: 1204 KISSFLR 1210
>NP_057518
          Length = 1219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RISSFLR 9
            +ISSFLR
Sbjct: 1180 KISSFLR 1186
>NP_891550
          Length = 1935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HFRISSF 7
           H+R+S+F
Sbjct: 99  HYRLSAF 105
>NP_570969
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RISSFLR 9
           +ISSFLR
Sbjct: 533 KISSFLR 539
>NP_006355
          Length = 765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R SSFL+
Sbjct: 583 HLRRSSFLQ 591
>XP_005267319
          Length = 789

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R SSFL+
Sbjct: 607 HLRRSSFLQ 615
>XP_005271451
          Length = 1000

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HFRISSFLR 9
           HF+ISS L+
Sbjct: 135 HFQISSQLQ 143
>XP_005271450
          Length = 1000

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HFRISSFLR 9
           HF+ISS L+
Sbjct: 135 HFQISSQLQ 143
>XP_005271449
          Length = 1000

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HFRISSFLR 9
           HF+ISS L+
Sbjct: 135 HFQISSQLQ 143
>XP_005271447
          Length = 1000

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HFRISSFLR 9
           HF+ISS L+
Sbjct: 135 HFQISSQLQ 143
>XP_005265386
          Length = 1907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HFRISSF 7
           H+R+S+F
Sbjct: 99  HYRLSAF 105
>NP_056471
          Length = 1882

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HFRISSF 7
           HFR S+F
Sbjct: 835 HFRCSTF 841
>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    FRISSF 7
            FR+SSF
Sbjct: 1490 FRVSSF 1495
>NP_009041
          Length = 2627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    HFRISSFL 8
            +FR SSFL
Sbjct: 1028 YFRDSSFL 1035
>NP_443128
          Length = 3487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RISSFLR 9
            +ISSFLR
Sbjct: 2081 QISSFLR 2087
>NP_004517
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   HFRISSFLR 9
           H R  +FLR
Sbjct: 196 HHRFKNFLR 204
>NP_689966
          Length = 993

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RISSFLR 9
           ++SSFLR
Sbjct: 984 KVSSFLR 990
>NP_056068
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  FRISSFLR 9
          FR+  FLR
Sbjct: 6  FRLQKFLR 13
>NP_689612
          Length = 130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HFRISSFL 8
           HF + SFL
Sbjct: 98  HFLLKSFL 105
>NP_055692
          Length = 1942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FRISSFLR 9
          +RI+SFL+
Sbjct: 62 YRIASFLQ 69
>NP_653191
          Length = 191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  HFRISSFL 8
          H RI SFL
Sbjct: 52 HVRIVSFL 59
>NP_006648
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R+SS L+
Sbjct: 515 HHRVSSLLQ 523
>NP_996848
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R+SS L+
Sbjct: 515 HHRVSSLLQ 523
>NP_060899
          Length = 2624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    HFRISS 6
            HF+ISS
Sbjct: 1383 HFKISS 1388
>XP_006725524
          Length = 2584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    HFRISS 6
            HF+ISS
Sbjct: 1383 HFKISS 1388
>XP_006725523
          Length = 2623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    HFRISS 6
            HF+ISS
Sbjct: 1383 HFKISS 1388
>XP_005276828
          Length = 2584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    HFRISS 6
            HF+ISS
Sbjct: 1383 HFKISS 1388
>XP_006726279
          Length = 2623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    HFRISS 6
            HF+ISS
Sbjct: 1383 HFKISS 1388
>XP_006724024
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   HFRISSFLR 9
           H R+SS L+
Sbjct: 558 HHRVSSLLQ 566
>XP_005257947
          Length = 1935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FRISSFLR 9
          +RI+SFL+
Sbjct: 62 YRIASFLQ 69
>XP_005257946
          Length = 1943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FRISSFLR 9
          +RI+SFL+
Sbjct: 62 YRIASFLQ 69
>XP_005257945
          Length = 1969

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FRISSFLR 9
          +RI+SFL+
Sbjct: 62 YRIASFLQ 69
>XP_006722277
          Length = 1970

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FRISSFLR 9
          +RI+SFL+
Sbjct: 62 YRIASFLQ 69
>XP_005256630
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  FRISSFLR 9
          FR+  FLR
Sbjct: 6  FRLQKFLR 13
>XP_005256629
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  FRISSFLR 9
          FR+  FLR
Sbjct: 6  FRLQKFLR 13
>XP_005268084
          Length = 2627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    HFRISSFL 8
            +FR SSFL
Sbjct: 1028 YFRDSSFL 1035
>XP_005249005
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RISSFLR 9
           ++SSFLR
Sbjct: 522 KVSSFLR 528
>XP_005249004
          Length = 955

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RISSFLR 9
           ++SSFLR
Sbjct: 946 KVSSFLR 952
>XP_005249003
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RISSFLR 9
           ++SSFLR
Sbjct: 978 KVSSFLR 984
>XP_005249000
          Length = 1009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    RISSFLR 9
            ++SSFLR
Sbjct: 1000 KVSSFLR 1006
>XP_006714722
          Length = 2584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    HFRISS 6
            HF+ISS
Sbjct: 1383 HFKISS 1388
>XP_006714721
          Length = 2623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    HFRISS 6
            HF+ISS
Sbjct: 1383 HFKISS 1388
>XP_005262797
          Length = 130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HFRISSFL 8
           HF + SFL
Sbjct: 98  HFLLKSFL 105
>XP_005262794
          Length = 130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   HFRISSFL 8
           HF + SFL
Sbjct: 98  HFLLKSFL 105
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.353    0.152    0.474 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 427,561
Number of extensions: 635
Number of successful extensions: 62
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 62
Number of HSP's successfully gapped: 62
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)

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