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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000236                                                              21     175
NP_001120972                                                           21     175
XP_006716054                                                           21     175
XP_006716053                                                           21     175
XP_006716052                                                           21     175
XP_006716051                                                           21     175
NP_065816                                                              17    3301
XP_006710573                                                           17    3301
XP_006710572                                                           17    3301
XP_006710571                                                           17    3301
XP_006710570                                                           17    3301
XP_006710569                                                           17    3301
XP_006710568                                                           17    3301
XP_006710567                                                           17    3301
XP_006710566                                                           17    3301
XP_005245859                                                           17    3301
NP_056998                                                              16    5631
NP_001167560                                                           16    5631
NP_114423                                                              16    5631
NP_001167561                                                           16    5631
XP_005260914                                                           16    5631
XP_005260913                                                           16    5631
XP_005255403                                                           16    5631
NP_060101                                                              16    7355
NP_056263                                                              15    9605
XP_006717808                                                           15    9605
NP_001005281                                                           15    9605

>NP_000236
          Length = 1390

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   HFNMSLIIS 9
           HFNMS+IIS
Sbjct: 633 HFNMSIIIS 641



>NP_001120972
          Length = 1408

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   HFNMSLIIS 9
           HFNMS+IIS
Sbjct: 633 HFNMSIIIS 641



>XP_006716054
          Length = 960

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   HFNMSLIIS 9
           HFNMS+IIS
Sbjct: 203 HFNMSIIIS 211



>XP_006716053
          Length = 960

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   HFNMSLIIS 9
           HFNMS+IIS
Sbjct: 203 HFNMSIIIS 211



>XP_006716052
          Length = 1409

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   HFNMSLIIS 9
           HFNMS+IIS
Sbjct: 652 HFNMSIIIS 660



>XP_006716051
          Length = 1427

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   HFNMSLIIS 9
           HFNMS+IIS
Sbjct: 652 HFNMSIIIS 660



>NP_065816
          Length = 5183

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710573
          Length = 5177

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710572
          Length = 5188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710571
          Length = 5194

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710570
          Length = 5195

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710569
          Length = 5212

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710568
          Length = 5221

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710567
          Length = 5222

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_006710566
          Length = 5222

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>XP_005245859
          Length = 5223

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           HFN SL+ S
Sbjct: 712 HFNHSLVTS 720



>NP_056998
          Length = 958

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HFNMSLI 7
           HFN SL+
Sbjct: 297 HFNTSLV 303



>NP_001167560
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FNMSLIIS 9
           FN+SL++S
Sbjct: 461 FNLSLVMS 468



>NP_114423
          Length = 891

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FNMSLIIS 9
           FN+SL++S
Sbjct: 477 FNLSLVMS 484



>NP_001167561
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FNMSLIIS 9
           FN+SL++S
Sbjct: 504 FNLSLVMS 511



>XP_005260914
          Length = 856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FNMSLIIS 9
           FN+SL++S
Sbjct: 442 FNLSLVMS 449



>XP_005260913
          Length = 998

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FNMSLIIS 9
           FN+SL++S
Sbjct: 584 FNLSLVMS 591



>XP_005255403
          Length = 744

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  HFNMSLI 7
          HFN SL+
Sbjct: 83 HFNTSLV 89



>NP_060101
          Length = 1712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HFNMSLII 8
            HFN  L+I
Sbjct: 1057 HFNNKLVI 1064



>NP_056263
          Length = 203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           H N +LIIS
Sbjct: 161 HANCTLIIS 169



>XP_006717808
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HFNMSLIIS 9
           H N +LIIS
Sbjct: 331 HANCTLIIS 339



>NP_001005281
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FNMSLIIS 9
           FNM+ IIS
Sbjct: 266 FNMNKIIS 273



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.140    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 350,702
Number of extensions: 380
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)