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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001231639                                                           17    2528
NP_002047                                                              17    2528
NP_001189404                                                           17    3301
NP_001874                                                              17    3301
NP_001189411                                                           17    3301
NP_001189410                                                           17    3301
XP_006715714                                                           17    3301
NP_004949                                                              16    5631
XP_005263496                                                           16    5631
XP_005263495                                                           16    5631
NP_699189                                                              16    7355
NP_078951                                                              15    9605
NP_937762                                                              15    9605
NP_001779                                                              15    9605
NP_001229885                                                           15    9605
NP_001011553                                                           15    9605
NP_001121190                                                           15    9605
NP_000218                                                              15    9605
NP_001121189                                                           15    9605
NP_061836                                                              15    9605
NP_663630                                                              15    9605
NP_387505                                                              15    9605
XP_005261051                                                           15    9605
XP_006715873                                                           15    9605
XP_006715872                                                           15    9605
XP_006715871                                                           15    9605
XP_006715870                                                           15    9605
XP_006715869                                                           15    9605

>NP_001231639
          Length = 699

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HAIKKTAG 8
           H IK+TAG
Sbjct: 310 HRIKRTAG 317



>NP_002047
          Length = 681

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   HAIKKTAG 8
           H IK+TAG
Sbjct: 292 HRIKRTAG 299



>NP_001189404
          Length = 438

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HAIKKTAGV 9
           H+IK+TA V
Sbjct: 430 HSIKQTAAV 438



>NP_001874
          Length = 411

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HAIKKTAGV 9
           H+IK+TA V
Sbjct: 403 HSIKQTAAV 411



>NP_001189411
          Length = 410

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HAIKKTAGV 9
           H+IK+TA V
Sbjct: 402 HSIKQTAAV 410



>NP_001189410
          Length = 397

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HAIKKTAGV 9
           H+IK+TA V
Sbjct: 389 HSIKQTAAV 397



>XP_006715714
          Length = 411

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   HAIKKTAGV 9
           H+IK+TA V
Sbjct: 403 HSIKQTAAV 411



>NP_004949
          Length = 2549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HAIKKTAG 8
            HA+  TAG
Sbjct: 2289 HAVNNTAG 2296



>XP_005263496
          Length = 2322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HAIKKTAG 8
            HA+  TAG
Sbjct: 2062 HAVNNTAG 2069



>XP_005263495
          Length = 2549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HAIKKTAG 8
            HA+  TAG
Sbjct: 2289 HAVNNTAG 2296



>NP_699189
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  KKTAGV 9
          KKTAGV
Sbjct: 92 KKTAGV 97



>NP_078951
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  HAIKKT 6
          H+IKKT
Sbjct: 46 HSIKKT 51



>NP_937762
          Length = 3333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    IKKTAGV 9
            +KKT+GV
Sbjct: 2741 LKKTSGV 2747



>NP_001779
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 85 HRIKKTVQV 93



>NP_001229885
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 49 HRIKKTVQV 57



>NP_001011553
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 84 HRIKKTVQV 92



>NP_001121190
          Length = 1668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    IKKTAGV 9
            +KKT+GV
Sbjct: 1076 LKKTSGV 1082



>NP_000218
          Length = 1724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    IKKTAGV 9
            +KKT+GV
Sbjct: 1132 LKKTSGV 1138



>NP_001121189
          Length = 3277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    IKKTAGV 9
            +KKT+GV
Sbjct: 2685 LKKTSGV 2691



>NP_061836
          Length = 2320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HAIKKTAG 8
            HA  +TAG
Sbjct: 1767 HACNRTAG 1774



>NP_663630
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HAIKKT 6
           HAIK+T
Sbjct: 224 HAIKRT 229



>NP_387505
          Length = 2269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HAIKKTAG 8
            HA  +TAG
Sbjct: 1767 HACNRTAG 1774



>XP_005261051
          Length = 2242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    HAIKKTAG 8
            HA  +TAG
Sbjct: 1689 HACNRTAG 1696



>XP_006715873
          Length = 427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 64 HRIKKTVQV 72



>XP_006715872
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 82 HRIKKTVQV 90



>XP_006715871
          Length = 446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 83 HRIKKTVQV 91



>XP_006715870
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 84 HRIKKTVQV 92



>XP_006715869
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  HAIKKTAGV 9
          H IKKT  V
Sbjct: 85 HRIKKTVQV 93



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.125    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 438,868
Number of extensions: 737
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)