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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1135
NP_003931                                                              16    7355
NP_066964                                                              15    9605
NP_001193947                                                           15    9605
NP_599031                                                              15    9605
NP_008863                                                              15    9605
NP_001193946                                                           15    9605
NP_599032                                                              15    9605
NP_061986                                                              15    9605
NP_001074960                                                           15    9605
NP_110395                                                              15    9605
NP_006353                                                              15    9605
XP_006724370                                                           15    9605
XP_006724369                                                           15    9605
XP_006724368                                                           15    9605
XP_006724367                                                           15    9605
XP_006724366                                                           15    9605
XP_006724365                                                           15    9605
XP_006724364                                                           15    9605
XP_006724363                                                           15    9605
XP_006724362                                                           15    9605
XP_006724361                                                           15    9605
XP_005258788                                                           15    9605
XP_006718483                                                           15    9605
XP_006718482                                                           15    9605
XP_006718481                                                           15    9605
XP_005246163                                                           15    9605
XP_006712847                                                           15    9605
XP_005246894                                                           15    9605
XP_005246893                                                           15    9605

>NP_443179
          Length = 2170

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GVRSSRFSK 9
           GVRSSR SK
Sbjct: 411 GVRSSRVSK 419



>NP_003931
          Length = 863

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GVRSSRFS 8
           GV++SRF+
Sbjct: 568 GVKTSRFA 575



>NP_066964
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 139 SSRFSK 144



>NP_001193947
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 446 SSRFSK 451



>NP_599031
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>NP_008863
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>NP_001193946
          Length = 1002

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 444 SSRFSK 449



>NP_599032
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>NP_061986
          Length = 209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GVRSSRF 7
           GV++SRF
Sbjct: 95  GVKTSRF 101



>NP_001074960
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GVRSSRFSK 9
          G+RSSR  +
Sbjct: 46 GIRSSRLEE 54



>NP_110395
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   GVRSSRFS 8
           G+R+S+F+
Sbjct: 156 GIRASKFA 163



>NP_006353
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GVRSSRFSK 9
          G+RSSR  +
Sbjct: 46 GIRSSRLEE 54



>XP_006724370
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_006724369
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 114 SSRFSK 119



>XP_006724368
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_006724367
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_006724366
          Length = 857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_006724365
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 302 SSRFSK 307



>XP_006724364
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_006724363
          Length = 872

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_006724362
          Length = 946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_006724361
          Length = 948

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 390 SSRFSK 395



>XP_005258788
          Length = 209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GVRSSRF 7
           GV++SRF
Sbjct: 95  GVKTSRF 101



>XP_006718483
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GVRSSRFSK 9
          G+RSSR  +
Sbjct: 46 GIRSSRLEE 54



>XP_006718482
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GVRSSRFSK 9
          G+RSSR  +
Sbjct: 46 GIRSSRLEE 54



>XP_006718481
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GVRSSRFSK 9
          G+RSSR  +
Sbjct: 46 GIRSSRLEE 54



>XP_005246163
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  GVRSSRFS 8
          G+R+S+F+
Sbjct: 36 GIRASKFA 43



>XP_006712847
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  GVRSSRFS 8
          G+R+S+F+
Sbjct: 88 GIRASKFA 95



>XP_005246894
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 234 SSRFSK 239



>XP_005246893
          Length = 827

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SSRFSK 9
           SSRFSK
Sbjct: 234 SSRFSK 239



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.126    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 328,777
Number of extensions: 518
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)