Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275955                                                           17    3301
XP_005258977                                                           17    3301
XP_006726221                                                           17    3301
XP_005277168                                                           17    3301
NP_001252506                                                           16    5631
NP_001251503                                                           16    5631
NP_057686                                                              16    5631
XP_006723106                                                           16    5631
XP_006723105                                                           16    5631
XP_005257134                                                           16    5631
NP_604393                                                              16    5631
NP_001004465                                                           16    5631
NP_039226                                                              16    5631
NP_064585                                                              16    7355
NP_001269665                                                           16    7355
NP_116201                                                              16    7355
NP_001269715                                                           16    7355
NP_054788                                                              16    7355
NP_444512                                                              16    7355
XP_005252595                                                           16    7355
XP_005252594                                                           16    7355
XP_006717543                                                           16    7355
XP_006717542                                                           16    7355
XP_006710375                                                           16    7355
XP_006710374                                                           16    7355
XP_005270464                                                           16    7355
XP_005270463                                                           16    7355
XP_005270462                                                           16    7355
XP_006710373                                                           16    7355
XP_005270461                                                           16    7355
NP_003954                                                              16    7355
NP_443724                                                              16    7355
NP_001269282                                                           15    9605
NP_000879                                                              15    9605
NP_001269318                                                           15    9605
NP_001171655                                                           15    9605
NP_001269317                                                           15    9605
NP_001269319                                                           15    9605
NP_000346                                                              15    9605
NP_001019572                                                           15    9605
NP_001269283                                                           15    9605
NP_001269284                                                           15    9605
XP_005255372                                                           15    9605
NP_006444                                                              15    9605
>NP_001275955
          Length = 280

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  MVLCVAQ 9
          MVLC+AQ
Sbjct: 5  MVLCLAQ 11
>XP_005258977
          Length = 281

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  MVLCVAQ 9
          MVLC+AQ
Sbjct: 5  MVLCLAQ 11
>XP_006726221
          Length = 281

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  MVLCVAQ 9
          MVLC+AQ
Sbjct: 5  MVLCLAQ 11
>XP_005277168
          Length = 281

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  MVLCVAQ 9
          MVLC+AQ
Sbjct: 5  MVLCLAQ 11
>NP_001252506
          Length = 852

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VLCVAQ 9
           VLCVAQ
Sbjct: 511 VLCVAQ 516
>NP_001251503
          Length = 833

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VLCVAQ 9
           VLCVAQ
Sbjct: 523 VLCVAQ 528
>NP_057686
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GVMVLCVA 8
           GVMV C A
Sbjct: 221 GVMVACYA 228
>XP_006723106
          Length = 971

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GVMVLCVA 8
           GV+VLC A
Sbjct: 914 GVVVLCEA 921
>XP_006723105
          Length = 1028

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GVMVLCVA 8
           GV+VLC A
Sbjct: 971 GVVVLCEA 978
>XP_005257134
          Length = 842

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VLCVAQ 9
           VLCVAQ
Sbjct: 532 VLCVAQ 537
>NP_604393
          Length = 994

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GVMVLCVA 8
           GV+VLC A
Sbjct: 937 GVVVLCEA 944
>NP_001004465
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GVM++CV
Sbjct: 200 GVMLVCV 206
>NP_039226
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GVM++CV
Sbjct: 200 GVMLVCV 206
>NP_064585
          Length = 225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VMVLCV 7
          +MVLCV
Sbjct: 69 IMVLCV 74
>NP_001269665
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GVMVLC 6
          GVM+LC
Sbjct: 13 GVMLLC 18
>NP_116201
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GVMVLC 6
          GVM+LC
Sbjct: 13 GVMLLC 18
>NP_001269715
          Length = 190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VMVLCV 7
          +MVLCV
Sbjct: 69 IMVLCV 74
>NP_054788
          Length = 136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  GVMVLCV 7
          G++VLC+
Sbjct: 9  GILVLCL 15
>NP_444512
          Length = 132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  GVMVLCVA 8
          GV+ LCV+
Sbjct: 38 GVLCLCVS 45
>XP_005252595
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VMVLCV 7
          +MVLCV
Sbjct: 52 IMVLCV 57
>XP_005252594
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VMVLCV 7
          +MVLCV
Sbjct: 52 IMVLCV 57
>XP_006717543
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VMVLCV 7
          +MVLCV
Sbjct: 56 IMVLCV 61
>XP_006717542
          Length = 213

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VMVLCV 7
          +MVLCV
Sbjct: 57 IMVLCV 62
>XP_006710375
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MVLCVA 8
         MVLC+A
Sbjct: 1 MVLCIA 6
>XP_006710374
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MVLCVA 8
         MVLC+A
Sbjct: 1 MVLCIA 6
>XP_005270464
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  MVLCVA 8
          MVLC+A
Sbjct: 57 MVLCIA 62
>XP_005270463
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  MVLCVA 8
          MVLC+A
Sbjct: 94 MVLCIA 99
>XP_005270462
          Length = 460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MVLCVA 8
           MVLC+A
Sbjct: 152 MVLCIA 157
>XP_006710373
          Length = 460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MVLCVA 8
           MVLC+A
Sbjct: 152 MVLCIA 157
>XP_005270461
          Length = 460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MVLCVA 8
           MVLC+A
Sbjct: 152 MVLCIA 157
>NP_003954
          Length = 197

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GVMVLC 6
          G+MVLC
Sbjct: 57 GLMVLC 62
>NP_443724
          Length = 460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MVLCVA 8
           MVLC+A
Sbjct: 152 MVLCIA 157
>NP_001269282
          Length = 788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GV++LC+
Sbjct: 723 GVVLLCI 729
>NP_000879
          Length = 788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GV++LC+
Sbjct: 723 GVVLLCI 729
>NP_001269318
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GV++LC+
Sbjct: 650 GVVLLCI 656
>NP_001171655
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GVMVLCVAQ 9
           G++ LC+ Q
Sbjct: 133 GILALCLHQ 141
>NP_001269317
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GV++LC+
Sbjct: 681 GVVLLCI 687
>NP_001269319
          Length = 650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GV++LC+
Sbjct: 585 GVVLLCI 591
>NP_000346
          Length = 427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GVMVLCVAQ 9
           G++ LC+ Q
Sbjct: 160 GILALCLHQ 168
>NP_001019572
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   GVMVLCV 7
           GV++ CV
Sbjct: 240 GVLIFCV 246
>NP_001269283
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GV++LC+
Sbjct: 628 GVVLLCI 634
>NP_001269284
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GVMVLCV 7
           GV++LC+
Sbjct: 616 GVVLLCI 622
>XP_005255372
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   GVMVLCV 7
           GV++ CV
Sbjct: 274 GVLIFCV 280
>NP_006444
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VLCVAQ 9
           V+CVAQ
Sbjct: 411 VMCVAQ 416
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.142    0.447 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 519,013
Number of extensions: 560
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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