Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     665
NP_000242                                                              19     665
XP_005264389                                                           19     665
NP_060060                                                              18    1935
XP_005250956                                                           17    3301
NP_000828                                                              17    3301
NP_001009184                                                           17    3301
NP_653251                                                              17    4312
NP_038479                                                              16    5631
NP_579899                                                              16    5631
NP_620417                                                              16    5631
NP_001171582                                                           16    5631
NP_001171583                                                           16    5631
XP_006720485                                                           16    5631
XP_006720484                                                           16    5631
XP_006720483                                                           16    5631
XP_006717823                                                           16    5631
XP_005269751                                                           16    5631
XP_005269750                                                           16    5631
NP_689501                                                              16    7355
NP_851322                                                              16    7355
NP_660159                                                              15    9605
NP_116317                                                              15    9605
NP_001028755                                                           15    9605
NP_055793                                                              15    9605
NP_001032248                                                           15    9605
NP_001275929                                                           15    9605
NP_001275930                                                           15    9605
NP_705837                                                              15    9605
NP_996531                                                              15    9605
XP_005274529                                                           15    9605
XP_005274528                                                           15    9605
XP_005274527                                                           15    9605
XP_006724226                                                           15    9605
XP_006724225                                                           15    9605
XP_005254088                                                           15    9605
XP_006719978                                                           15    9605
XP_005254087                                                           15    9605
XP_006715412                                                           15    9605
XP_005247166                                                           15    9605
NP_073623                                                              15    9605
NP_001073909                                                           15    9605
NP_001004684                                                           15    9605
NP_001004692                                                           15    9605
>NP_001245210
          Length = 868

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GVFTFMAEM 9
           GV TFMAEM
Sbjct: 655 GVSTFMAEM 663
>NP_000242
          Length = 934

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GVFTFMAEM 9
           GV TFMAEM
Sbjct: 721 GVSTFMAEM 729
>XP_005264389
          Length = 921

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GVFTFMAEM 9
           GV TFMAEM
Sbjct: 721 GVSTFMAEM 729
>NP_060060
          Length = 977

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GVFTFMAEM 9
           G+FTF+ E+
Sbjct: 548 GIFTFLCEI 556
>XP_005250956
          Length = 371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VFTFMAEM 9
           VFTF+AE+
Sbjct: 182 VFTFVAEV 189
>NP_000828
          Length = 371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VFTFMAEM 9
           VFTF+AE+
Sbjct: 182 VFTFVAEV 189
>NP_001009184
          Length = 371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VFTFMAEM 9
           VFTF+AE+
Sbjct: 182 VFTFVAEV 189
>NP_653251
          Length = 467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VFTFMAEM 9
           VFTF A+M
Sbjct: 368 VFTFTAQM 375
>NP_038479
          Length = 2061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GVFTFMAEM 9
           G FT  AEM
Sbjct: 855 GTFTVFAEM 863
>NP_579899
          Length = 2048

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GVFTFMAEM 9
           G FT  AEM
Sbjct: 842 GTFTVFAEM 850
>NP_620417
          Length = 235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FTFMAEM 9
           FTFMA++
Sbjct: 110 FTFMAQL 116
>NP_001171582
          Length = 235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FTFMAEM 9
           FTFMA++
Sbjct: 110 FTFMAQL 116
>NP_001171583
          Length = 235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FTFMAEM 9
           FTFMA++
Sbjct: 110 FTFMAQL 116
>XP_006720485
          Length = 164

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FTFMAEM 9
          FTFMA++
Sbjct: 39 FTFMAQL 45
>XP_006720484
          Length = 235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FTFMAEM 9
           FTFMA++
Sbjct: 110 FTFMAQL 116
>XP_006720483
          Length = 253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FTFMAEM 9
           FTFMA++
Sbjct: 128 FTFMAQL 134
>XP_006717823
          Length = 1938

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GVFTFMAEM 9
           G FT  AEM
Sbjct: 713 GTFTVFAEM 721
>XP_005269751
          Length = 2067

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GVFTFMAEM 9
           G FT  AEM
Sbjct: 842 GTFTVFAEM 850
>XP_005269750
          Length = 2080

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GVFTFMAEM 9
           G FT  AEM
Sbjct: 855 GTFTVFAEM 863
>NP_689501
          Length = 295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GVFTFMAEM 9
           G F F+AE+
Sbjct: 158 GTFLFLAEV 166
>NP_851322
          Length = 724

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VFTFMA 7
           +FTFMA
Sbjct: 589 IFTFMA 594
>NP_660159
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  GVFTFMAEM 9
          G+FTF++ +
Sbjct: 37 GMFTFLSSV 45
>NP_116317
          Length = 108

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GVFTFM 6
          GVFTF+
Sbjct: 34 GVFTFV 39
>NP_001028755
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FTFMAE 8
           FTFM+E
Sbjct: 393 FTFMSE 398
>NP_055793
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FTFMAE 8
           FTF+AE
Sbjct: 967 FTFLAE 972
>NP_001032248
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FTFMAE 8
           FTFM+E
Sbjct: 402 FTFMSE 407
>NP_001275929
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   GVFTFMAE 8
           G FTF  E
Sbjct: 523 GTFTFQCE 530
>NP_001275930
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   GVFTFMAE 8
           G FTF  E
Sbjct: 523 GTFTFQCE 530
>NP_705837
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   GVFTFMAE 8
           G FTF  E
Sbjct: 532 GTFTFQCE 539
>NP_996531
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   GVFTFMAEM 9
           G FTF   M
Sbjct: 310 GAFTFQGSM 318
>XP_005274529
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FTFMAE 8
           FTFM+E
Sbjct: 333 FTFMSE 338
>XP_005274528
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FTFMAE 8
           FTFM+E
Sbjct: 333 FTFMSE 338
>XP_005274527
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FTFMAE 8
           FTFM+E
Sbjct: 405 FTFMSE 410
>XP_006724226
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   GVFTFMAE 8
           G FTF  E
Sbjct: 523 GTFTFQCE 530
>XP_006724225
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   GVFTFMAE 8
           G FTF  E
Sbjct: 523 GTFTFQCE 530
>XP_005254088
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   GVFTFMAEM 9
           G FTF   M
Sbjct: 276 GAFTFQGSM 284
>XP_006719978
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   GVFTFMAEM 9
           G FTF   M
Sbjct: 310 GAFTFQGSM 318
>XP_005254087
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   GVFTFMAEM 9
           G FTF   M
Sbjct: 310 GAFTFQGSM 318
>XP_006715412
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  GVFTFMAEM 9
          G+FTF++ +
Sbjct: 37 GMFTFLSSV 45
>XP_005247166
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VFTFMAEM 9
          +FT MAEM
Sbjct: 58 LFTEMAEM 65
>NP_073623
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   GVFTFM 6
           G+FTF+
Sbjct: 157 GIFTFL 162
>NP_001073909
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VFTFMAEM 9
          +FT MAEM
Sbjct: 58 LFTEMAEM 65
>NP_001004684
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   GVFTFM 6
           G+FT+M
Sbjct: 271 GIFTYM 276
>NP_001004692
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   GVFTFM 6
           G+FT+M
Sbjct: 253 GIFTYM 258
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.143    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,096
Number of extensions: 383
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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