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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_006723199                                                           17    4312
XP_006723198                                                           17    4312
XP_006723197                                                           17    4312
XP_006723196                                                           17    4312
NP_056418                                                              17    4312
NP_001239049                                                           16    5631
NP_055963                                                              16    5631
NP_001239053                                                           16    5631
NP_001239051                                                           16    5631
NP_001239048                                                           16    5631
XP_003960784                                                           16    5631
XP_005276355                                                           16    5631
XP_938432                                                              16    5631
XP_005250801                                                           16    5631
XP_005247048                                                           16    5631
XP_005247047                                                           16    5631
XP_005275869                                                           16    5631
XP_005276060                                                           16    5631
NP_001924                                                              16    7355
NP_036342                                                              15    9605
NP_001093149                                                           15    9605
NP_001243210                                                           15    9605
NP_001164015                                                           15    9605
NP_443141                                                              15    9605
NP_612490                                                              15    9605
NP_072174                                                              15    9605
NP_006050                                                              15    9605
NP_612642                                                              15    9605
NP_001164014                                                           15    9605
XP_005261428                                                           15    9605
XP_005261427                                                           15    9605
XP_005251870                                                           15    9605
XP_006716984                                                           15    9605
XP_006714509                                                           15    9605
XP_006712785                                                           15    9605
XP_005246883                                                           15    9605
XP_005246882                                                           15    9605
XP_006712784                                                           15    9605

>XP_006723199
          Length = 505

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GVAVASPR 8
          GVAV +PR
Sbjct: 49 GVAVTAPR 56



>XP_006723198
          Length = 587

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GVAVASPR 8
          GVAV +PR
Sbjct: 49 GVAVTAPR 56



>XP_006723197
          Length = 587

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GVAVASPR 8
          GVAV +PR
Sbjct: 49 GVAVTAPR 56



>XP_006723196
          Length = 587

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GVAVASPR 8
          GVAV +PR
Sbjct: 49 GVAVTAPR 56



>NP_056418
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GVAVASPR 8
          GVAV +PR
Sbjct: 49 GVAVTAPR 56



>NP_001239049
          Length = 1323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAVASPRA 9
           G AVA P+A
Sbjct: 589 GAAVAKPQA 597



>NP_055963
          Length = 1323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAVASPRA 9
           G AVA P+A
Sbjct: 589 GAAVAKPQA 597



>NP_001239053
          Length = 1143

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAVASPRA 9
           G AVA P+A
Sbjct: 589 GAAVAKPQA 597



>NP_001239051
          Length = 1288

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAVASPRA 9
           G AVA P+A
Sbjct: 554 GAAVAKPQA 562



>NP_001239048
          Length = 1330

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAVASPRA 9
           G AVA P+A
Sbjct: 589 GAAVAKPQA 597



>XP_003960784
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VAVASPRA 9
           VAVA+P+A
Sbjct: 173 VAVAAPKA 180



>XP_005276355
          Length = 233

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  GVAVASP 7
          G+AVASP
Sbjct: 38 GLAVASP 44



>XP_938432
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VAVASPRA 9
           VAVA+P+A
Sbjct: 173 VAVAAPKA 180



>XP_005250801
          Length = 233

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  GVAVASP 7
          G+AVASP
Sbjct: 38 GLAVASP 44



>XP_005247048
          Length = 1107

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAVASPRA 9
           G AVA P+A
Sbjct: 373 GAAVAKPQA 381



>XP_005247047
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAVASPRA 9
           G AVA P+A
Sbjct: 403 GAAVAKPQA 411



>XP_005275869
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VAVASPRA 9
           VAVA+P+A
Sbjct: 173 VAVAAPKA 180



>XP_005276060
          Length = 233

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  GVAVASP 7
          G+AVASP
Sbjct: 38 GLAVASP 44



>NP_001924
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VAVASPR 8
           VAVA+PR
Sbjct: 319 VAVATPR 325



>NP_036342
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VASPRA 9
           VASPRA
Sbjct: 333 VASPRA 338



>NP_001093149
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VASPRA 9
           VASPRA
Sbjct: 333 VASPRA 338



>NP_001243210
          Length = 198

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VAVASPR 8
          V VASPR
Sbjct: 92 VVVASPR 98



>NP_001164015
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VAVASPRA 9
          VAV  PRA
Sbjct: 26 VAVGKPRA 33



>NP_443141
          Length = 1271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GVAVASPRA 9
           GVAV  P A
Sbjct: 568 GVAVLKPHA 576



>NP_612490
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AVASPRA 9
           AVA PRA
Sbjct: 372 AVAGPRA 378



>NP_072174
          Length = 1735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    AVASPRA 9
            AVA PRA
Sbjct: 1033 AVAGPRA 1039



>NP_006050
          Length = 1575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GVAVASPRA 9
          G+A+ +PRA
Sbjct: 9  GLALLAPRA 17



>NP_612642
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VAVASPRA 9
          VAV  PRA
Sbjct: 26 VAVGKPRA 33



>NP_001164014
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VAVASPRA 9
          VAV  PRA
Sbjct: 26 VAVGKPRA 33



>XP_005261428
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AVASPRA 9
           AVA PRA
Sbjct: 372 AVAGPRA 378



>XP_005261427
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AVASPRA 9
           AVA PRA
Sbjct: 372 AVAGPRA 378



>XP_005251870
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VASPRA 9
           VASPRA
Sbjct: 333 VASPRA 338



>XP_006716984
          Length = 1259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GVAVASPRA 9
          G+A+ +PRA
Sbjct: 9  GLALLAPRA 17



>XP_006714509
          Length = 1627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GVAVASPRA 9
           GVAV  P A
Sbjct: 924 GVAVLKPHA 932



>XP_006712785
          Length = 1352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AVASPRA 9
           AVA PRA
Sbjct: 650 AVAGPRA 656



>XP_005246883
          Length = 1352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AVASPRA 9
           AVA PRA
Sbjct: 650 AVAGPRA 656



>XP_005246882
          Length = 1518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AVASPRA 9
           AVA PRA
Sbjct: 816 AVAGPRA 822



>XP_006712784
          Length = 1784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    AVASPRA 9
            AVA PRA
Sbjct: 1082 AVAGPRA 1088



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.122    0.333 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 709,174
Number of extensions: 997
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)