Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
XP_005267290                                                           19     665
NP_003230                                                              17    3301
XP_005268085                                                           17    3301
XP_005254804                                                           17    4312
XP_006720791                                                           17    4312
XP_005254803                                                           17    4312
XP_005254800                                                           17    4312
XP_005254799                                                           17    4312
NP_003913                                                              17    4312
NP_071760                                                              16    5631
NP_689752                                                              16    5631
NP_078963                                                              16    7355
NP_001177203                                                           16    7355
NP_079409                                                              16    7355
NP_001186131                                                           16    7355
NP_001186130                                                           16    7355
NP_055134                                                              16    7355
NP_001161086                                                           16    7355
XP_005247836                                                           16    7355
XP_006713811                                                           16    7355
XP_006713810                                                           16    7355
XP_005247834                                                           16    7355
XP_005264631                                                           16    7355
XP_005264630                                                           16    7355
XP_005264629                                                           16    7355
NP_001116856                                                           15    9605
NP_001116855                                                           15    9605
NP_060215                                                              15    9605
NP_001116857                                                           15    9605
NP_065145                                                              15    9605
XP_005272677                                                           15    9605
XP_005272676                                                           15    9605
XP_005272675                                                           15    9605
XP_005272674                                                           15    9605
XP_006724599                                                           15    9605
XP_005272673                                                           15    9605

>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  GVAEQNELS 9
          GVAEQ+ELS
Sbjct: 67 GVAEQDELS 75



>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  GVAEQNELS 9
          GVAEQ+ELS
Sbjct: 67 GVAEQDELS 75



>NP_003230
          Length = 412

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GVAEQNELS 9
           G+AE NEL+
Sbjct: 113 GLAEHNELA 121



>XP_005268085
          Length = 412

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GVAEQNELS 9
           G+AE NEL+
Sbjct: 113 GLAEHNELA 121



>XP_005254804
          Length = 4796

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GVAEQNEL 8
            G+AE+N+L
Sbjct: 3254 GIAEENKL 3261



>XP_006720791
          Length = 4853

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GVAEQNEL 8
            G+AE+N+L
Sbjct: 3311 GIAEENKL 3318



>XP_005254803
          Length = 4854

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GVAEQNEL 8
            G+AE+N+L
Sbjct: 3312 GIAEENKL 3319



>XP_005254800
          Length = 4861

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GVAEQNEL 8
            G+AE+N+L
Sbjct: 3319 GIAEENKL 3326



>XP_005254799
          Length = 4861

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GVAEQNEL 8
            G+AE+N+L
Sbjct: 3319 GIAEENKL 3326



>NP_003913
          Length = 4861

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GVAEQNEL 8
            G+AE+N+L
Sbjct: 3319 GIAEENKL 3326



>NP_071760
          Length = 554

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           +AEQNE S
Sbjct: 473 IAEQNESS 480



>NP_689752
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GVAEQN 6
          GVAEQN
Sbjct: 74 GVAEQN 79



>NP_078963
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           + +QNELS
Sbjct: 681 ITDQNELS 688



>NP_001177203
          Length = 927

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GVAEQNELS 9
           G+ E NE+S
Sbjct: 421 GIYEDNEIS 429



>NP_079409
          Length = 843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GVAEQNELS 9
           G+ E NE+S
Sbjct: 337 GIYEDNEIS 345



>NP_001186131
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           + +QNELS
Sbjct: 652 ITDQNELS 659



>NP_001186130
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           + +QNELS
Sbjct: 720 ITDQNELS 727



>NP_055134
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAEQNELS 9
           GV+E  ELS
Sbjct: 499 GVSEDGELS 507



>NP_001161086
          Length = 910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GVAEQNELS 9
           GV+E  ELS
Sbjct: 499 GVSEDGELS 507



>XP_005247836
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           + +QNELS
Sbjct: 508 ITDQNELS 515



>XP_006713811
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           + +QNELS
Sbjct: 681 ITDQNELS 688



>XP_006713810
          Length = 838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           + +QNELS
Sbjct: 719 ITDQNELS 726



>XP_005247834
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAEQNELS 9
           + +QNELS
Sbjct: 720 ITDQNELS 727



>XP_005264631
          Length = 926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GVAEQNELS 9
           G+ E NE+S
Sbjct: 420 GIYEDNEIS 428



>XP_005264630
          Length = 953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GVAEQNELS 9
           G+ E NE+S
Sbjct: 420 GIYEDNEIS 428



>XP_005264629
          Length = 954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GVAEQNELS 9
           G+ E NE+S
Sbjct: 421 GIYEDNEIS 429



>NP_001116856
          Length = 1703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1053 IAEQNE 1058



>NP_001116855
          Length = 1721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1071 IAEQNE 1076



>NP_060215
          Length = 1721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1071 IAEQNE 1076



>NP_001116857
          Length = 1755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1071 IAEQNE 1076



>NP_065145
          Length = 199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AEQNELS 9
           AEQ+ELS
Sbjct: 183 AEQDELS 189



>XP_005272677
          Length = 1703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1053 IAEQNE 1058



>XP_005272676
          Length = 1737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1053 IAEQNE 1058



>XP_005272675
          Length = 1755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1071 IAEQNE 1076



>XP_005272674
          Length = 1755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1071 IAEQNE 1076



>XP_006724599
          Length = 1755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1071 IAEQNE 1076



>XP_005272673
          Length = 1755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VAEQNE 7
            +AEQNE
Sbjct: 1071 IAEQNE 1076



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290    0.114    0.271 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 426,244
Number of extensions: 464
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)