Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1482
NP_001263689                                                           18    1482
NP_001119584                                                           18    1482
NP_001119586                                                           18    1482
NP_001263625                                                           18    1482
NP_001119590                                                           18    1482
NP_001263690                                                           18    1482
NP_001119585                                                           18    1482
NP_001263624                                                           18    1482
NP_001119588                                                           18    1482
NP_001119587                                                           18    1482
NP_001119589                                                           18    1482
NP_003171                                                              16    7355
XP_006723405                                                           16    7355
XP_006723404                                                           16    7355
XP_006723403                                                           16    7355
XP_006723402                                                           16    7355
XP_006723401                                                           16    7355
NP_110380                                                              15    9605
NP_001159403                                                           15    9605
NP_001025                                                              15    9605
NP_001244202                                                           15    9605
>NP_000537
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001263689
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119584
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001119586
          Length = 341

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001263625
          Length = 302

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119590
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001263690
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119585
          Length = 346

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001263624
          Length = 307

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVLAMAI 9
           GTRV AMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119588
          Length = 209

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRVLAMAI 9
          GTRV AMAI
Sbjct: 22 GTRVRAMAI 30
>NP_001119587
          Length = 261

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRVLAMAI 9
          GTRV AMAI
Sbjct: 22 GTRVRAMAI 30
>NP_001119589
          Length = 214

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRVLAMAI 9
          GTRV AMAI
Sbjct: 22 GTRVRAMAI 30
>NP_003171
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVLAMAI 9
           G RVL MA+
Sbjct: 187 GGRVLVMAV 195
>XP_006723405
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVLAMAI 9
           G RVL MA+
Sbjct: 184 GGRVLVMAV 192
>XP_006723404
          Length = 383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVLAMAI 9
           G RVL MA+
Sbjct: 184 GGRVLVMAV 192
>XP_006723403
          Length = 385

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVLAMAI 9
           G RVL MA+
Sbjct: 187 GGRVLVMAV 195
>XP_006723402
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVLAMAI 9
           G RVL MA+
Sbjct: 187 GGRVLVMAV 195
>XP_006723401
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVLAMAI 9
           G RVL MA+
Sbjct: 187 GGRVLVMAV 195
>NP_110380
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GTRVLA 6
          GTRVLA
Sbjct: 16 GTRVLA 21
>NP_001159403
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRVLA 6
           GTRVLA
Sbjct: 99  GTRVLA 104
>NP_001025
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GTRVLA 6
          GTRVLA
Sbjct: 39 GTRVLA 44
>NP_001244202
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RVLAMAI 9
           RV+AMAI
Sbjct: 447 RVMAMAI 453
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.139    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 213,446
Number of extensions: 122
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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