Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              19     869
NP_001263689                                                           19     869
NP_001119584                                                           19     869
NP_001119586                                                           19     869
NP_001263625                                                           19     869
NP_001119590                                                           19     869
NP_001263690                                                           19     869
NP_001119585                                                           19     869
NP_001263624                                                           19     869
NP_001119588                                                           19     869
NP_001119587                                                           19     869
NP_001119589                                                           19     869
NP_079410                                                              17    4312
XP_006723706                                                           17    4312
XP_006723701                                                           17    4312
XP_006723700                                                           17    4312
XP_006723699                                                           17    4312
XP_006723698                                                           17    4312
XP_006723697                                                           17    4312
XP_006723696                                                           17    4312
XP_006723695                                                           17    4312
XP_005256233                                                           17    4312
XP_005256232                                                           17    4312
XP_005256231                                                           17    4312
XP_005256229                                                           17    4312
XP_005256228                                                           17    4312
XP_005256227                                                           17    4312
XP_006721346                                                           17    4312
XP_005256226                                                           17    4312
XP_006721345                                                           17    4312
XP_006721344                                                           17    4312
XP_006721343                                                           17    4312
XP_005256225                                                           17    4312
NP_055064                                                              16    5631
NP_000869                                                              16    5631
NP_057430                                                              16    5631
NP_001265543                                                           16    7355
NP_003352                                                              16    7355
NP_001008390                                                           16    7355
NP_277042                                                              16    7355
NP_277043                                                              16    7355
NP_000153                                                              16    7355
NP_066988                                                              16    7355
NP_003310                                                              16    7355
NP_597676                                                              16    7355
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
XP_006721151                                                           16    7355
XP_006721150                                                           16    7355
XP_006721149                                                           16    7355
XP_006721148                                                           16    7355
XP_005246888                                                           16    7355
XP_006712788                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
XP_005245436                                                           16    7355
NP_001254479                                                           16    7355
NP_597681                                                              16    7355
NP_115495                                                              15    9605
NP_954714                                                              15    9605
NP_077271                                                              15    9605
NP_055587                                                              15    9605
NP_001135869                                                           15    9605
NP_001078945                                                           15    9605
NP_001119806                                                           15    9605
NP_001128143                                                           15    9605
XP_006722652                                                           15    9605
XP_006722651                                                           15    9605
XP_006722650                                                           15    9605
XP_006722649                                                           15    9605
XP_006722648                                                           15    9605
XP_006722647                                                           15    9605
XP_006722646                                                           15    9605
XP_005258449                                                           15    9605
XP_006716720                                                           15    9605
NP_954586                                                              15    9605
>NP_000537
          Length = 393

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001263689
          Length = 354

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119584
          Length = 393

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001119586
          Length = 341

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001263625
          Length = 302

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119590
          Length = 354

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001263690
          Length = 354

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119585
          Length = 346

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 154 GTRVRAMAI 162
>NP_001263624
          Length = 307

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTR RAMAI
Sbjct: 115 GTRVRAMAI 123
>NP_001119588
          Length = 209

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRFRAMAI 9
          GTR RAMAI
Sbjct: 22 GTRVRAMAI 30
>NP_001119587
          Length = 261

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRFRAMAI 9
          GTR RAMAI
Sbjct: 22 GTRVRAMAI 30
>NP_001119589
          Length = 214

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRFRAMAI 9
          GTR RAMAI
Sbjct: 22 GTRVRAMAI 30
>NP_079410
          Length = 2881

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_006723706
          Length = 560

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 417 GTRFRA 422
>XP_006723701
          Length = 863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 397 GTRFRA 402
>XP_006723700
          Length = 869

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 403 GTRFRA 408
>XP_006723699
          Length = 870

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 404 GTRFRA 409
>XP_006723698
          Length = 876

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 410 GTRFRA 415
>XP_006723697
          Length = 882

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 416 GTRFRA 421
>XP_006723696
          Length = 882

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 417 GTRFRA 422
>XP_006723695
          Length = 883

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRFRA
Sbjct: 417 GTRFRA 422
>XP_005256233
          Length = 2391

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1360 TKFRAMA 1366
>XP_005256232
          Length = 2424

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1393 TKFRAMA 1399
>XP_005256231
          Length = 2573

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_005256229
          Length = 2881

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_005256228
          Length = 2882

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_005256227
          Length = 2897

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_006721346
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_005256226
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_006721345
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_006721344
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_006721343
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>XP_005256225
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TRFRAMA 8
            T+FRAMA
Sbjct: 1867 TKFRAMA 1873
>NP_055064
          Length = 410

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TRFRAMAI 9
           +RFRA+A+
Sbjct: 308 SRFRALAV 315
>NP_000869
          Length = 551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRFRAMAI 9
           G R+R MAI
Sbjct: 113 GVRWRVMAI 121
>NP_057430
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TRFRAMAI 9
           +RFRA+A+
Sbjct: 308 SRFRALAV 315
>NP_001265543
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTRFR+ ++
Sbjct: 617 GTRFRSGSV 625
>NP_003352
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTRFR+ ++
Sbjct: 584 GTRFRSGSV 592
>NP_001008390
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTRFR+ ++
Sbjct: 584 GTRFRSGSV 592
>NP_277042
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GTRFRAMAI 9
          GT FR M +
Sbjct: 82 GTNFRVMLV 90
>NP_277043
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GTRFRAMAI 9
          GT FR M +
Sbjct: 80 GTNFRVMLV 88
>NP_000153
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GTRFRAMAI 9
          GT FR M +
Sbjct: 81 GTNFRVMLV 89
>NP_066988
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GTRFRAM 7
          GTRFR +
Sbjct: 6  GTRFRGL 12
>NP_003310
          Length = 26926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RFRAMAI 9
            +FRAMAI
Sbjct: 9178 QFRAMAI 9184
>NP_597676
          Length = 27051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RFRAMAI 9
            +FRAMAI
Sbjct: 9303 QFRAMAI 9309
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3     RFRAMAI 9
             +FRAMAI
Sbjct: 16602 QFRAMAI 16608
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3     RFRAMAI 9
             +FRAMAI
Sbjct: 15675 QFRAMAI 15681
>XP_006721151
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTRFR+ ++
Sbjct: 584 GTRFRSGSV 592
>XP_006721150
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTRFR+ ++
Sbjct: 584 GTRFRSGSV 592
>XP_006721149
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTRFR+ ++
Sbjct: 584 GTRFRSGSV 592
>XP_006721148
          Length = 668

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GTRFR+ ++
Sbjct: 612 GTRFRSGSV 620
>XP_005246888
          Length = 22663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RFRAMAI 9
            +FRAMAI
Sbjct: 4915 QFRAMAI 4921
>XP_006712788
          Length = 24022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RFRAMAI 9
            +FRAMAI
Sbjct: 6274 QFRAMAI 6280
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3     RFRAMAI 9
             +FRAMAI
Sbjct: 16230 QFRAMAI 16236
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3     RFRAMAI 9
             +FRAMAI
Sbjct: 17736 QFRAMAI 17742
>XP_005245436
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GTRFRAM 7
          GTRFR +
Sbjct: 6  GTRFRGL 12
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3     RFRAMAI 9
             +FRAMAI
Sbjct: 18243 QFRAMAI 18249
>NP_597681
          Length = 27118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RFRAMAI 9
            +FRAMAI
Sbjct: 9370 QFRAMAI 9376
>NP_115495
          Length = 6306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GTRFRAMAI 9
            G RF+A+ I
Sbjct: 3145 GVRFKALQI 3153
>NP_954714
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GTRFRA 6
          GT+FRA
Sbjct: 87 GTKFRA 92
>NP_077271
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRF+A
Sbjct: 147 GTRFKA 152
>NP_055587
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 445 GTKFRSLLL 453
>NP_001135869
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 447 GTKFRSLLL 455
>NP_001078945
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GTRFRA 6
          GT+FRA
Sbjct: 87 GTKFRA 92
>NP_001119806
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GTRFRA 6
          GT+FRA
Sbjct: 87 GTKFRA 92
>NP_001128143
          Length = 231

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GTRFRA 6
           GTRF+A
Sbjct: 147 GTRFKA 152
>XP_006722652
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 447 GTKFRSLLL 455
>XP_006722651
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 447 GTKFRSLLL 455
>XP_006722650
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 447 GTKFRSLLL 455
>XP_006722649
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 461 GTKFRSLLL 469
>XP_006722648
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 463 GTKFRSLLL 471
>XP_006722647
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 463 GTKFRSLLL 471
>XP_006722646
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 463 GTKFRSLLL 471
>XP_005258449
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GTRFRAMAI 9
           GT+FR++ +
Sbjct: 463 GTKFRSLLL 471
>XP_006716720
          Length = 151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GTRFRA 6
          GTRF+A
Sbjct: 47 GTRFKA 52
>NP_954586
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GTRFRA 6
          GT+FRA
Sbjct: 87 GTKFRA 92
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.144    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 272,549
Number of extensions: 379
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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