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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1482
NP_001263689                                                           18    1482
NP_001119584                                                           18    1482
NP_001119586                                                           18    1482
NP_001263625                                                           18    1482
NP_001119590                                                           18    1482
NP_001263690                                                           18    1482
NP_001119585                                                           18    1482
NP_001263624                                                           18    1482
NP_001119588                                                           18    1482
NP_001119587                                                           18    1482
NP_001119589                                                           18    1482
NP_079225                                                              17    4312
XP_006714376                                                           17    4312
XP_006714375                                                           17    4312
NP_068659                                                              16    5631
NP_068658                                                              16    5631
NP_004349                                                              16    5631
NP_000862                                                              16    5631
NP_079085                                                              16    7355
NP_001137322                                                           16    7355
NP_689929                                                              16    7355
XP_005253680                                                           16    7355
XP_006719668                                                           16    7355
XP_005273891                                                           16    7355
XP_005273890                                                           16    7355
NP_065897                                                              15    9605
NP_001274324                                                           15    9605
XP_005248178                                                           15    9605
XP_006712547                                                           15    9605
NP_612635                                                              15    9605

>NP_000537
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 154 GTRVRAMAI 162



>NP_001263689
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 115 GTRVRAMAI 123



>NP_001119584
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 154 GTRVRAMAI 162



>NP_001119586
          Length = 341

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 154 GTRVRAMAI 162



>NP_001263625
          Length = 302

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 115 GTRVRAMAI 123



>NP_001119590
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 115 GTRVRAMAI 123



>NP_001263690
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 115 GTRVRAMAI 123



>NP_001119585
          Length = 346

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 154 GTRVRAMAI 162



>NP_001263624
          Length = 307

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTPVRAMAI 9
           GT VRAMAI
Sbjct: 115 GTRVRAMAI 123



>NP_001119588
          Length = 209

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTPVRAMAI 9
          GT VRAMAI
Sbjct: 22 GTRVRAMAI 30



>NP_001119587
          Length = 261

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTPVRAMAI 9
          GT VRAMAI
Sbjct: 22 GTRVRAMAI 30



>NP_001119589
          Length = 214

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTPVRAMAI 9
          GT VRAMAI
Sbjct: 22 GTRVRAMAI 30



>NP_079225
          Length = 1192

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTPVRAMA 8
           GTP RA+A
Sbjct: 390 GTPSRALA 397



>XP_006714376
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTPVRAMA 8
           GTP RA+A
Sbjct: 390 GTPSRALA 397



>XP_006714375
          Length = 1208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTPVRAMA 8
           GTP RA+A
Sbjct: 390 GTPSRALA 397



>NP_068659
          Length = 580

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GTPVRAMA 8
          G+PVRA A
Sbjct: 41 GSPVRAAA 48



>NP_068658
          Length = 539

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GTPVRAMA 8
          G+PVRA A
Sbjct: 41 GSPVRAAA 48



>NP_004349
          Length = 566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GTPVRAMA 8
          G+PVRA A
Sbjct: 41 GSPVRAAA 48



>NP_000862
          Length = 440

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TPVRAMAI 9
           TP+RA+A+
Sbjct: 139 TPLRALAL 146



>NP_079085
          Length = 697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTPVRAMAI 9
           G PVRA+ I
Sbjct: 442 GKPVRALNI 450



>NP_001137322
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTPVRAMAI 9
           G PVRA+ I
Sbjct: 441 GKPVRALNI 449



>NP_689929
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTPVRAMAI 9
           GTP + MA+
Sbjct: 193 GTPPKQMAV 201



>XP_005253680
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTPVRAMAI 9
           G PVRA+ I
Sbjct: 397 GKPVRALNI 405



>XP_006719668
          Length = 662

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTPVRAMAI 9
           G PVRA+ I
Sbjct: 442 GKPVRALNI 450



>XP_005273891
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTPVRAMAI 9
           GTP + MA+
Sbjct: 193 GTPPKQMAV 201



>XP_005273890
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTPVRAMAI 9
           GTP + MA+
Sbjct: 193 GTPPKQMAV 201



>NP_065897
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GTPVRAMA 8
          G+PVRA A
Sbjct: 47 GSPVRAGA 54



>NP_001274324
          Length = 1688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TPVRAMAI 9
           +PVRA+A+
Sbjct: 873 SPVRALAM 880



>XP_005248178
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GTPVRAMA 8
          G+PVRA A
Sbjct: 47 GSPVRAGA 54



>XP_006712547
          Length = 1674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TPVRAMAI 9
           +PVRA+A+
Sbjct: 870 SPVRALAM 877



>NP_612635
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GTPVRAMA 8
           G P+RA+A
Sbjct: 131 GPPLRALA 138



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 568,211
Number of extensions: 367
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)