Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              19     665
XP_006715292                                                           19     665
NP_055616                                                              18    1935
XP_006711879                                                           18    1935
XP_005273329                                                           18    1935
NP_060660                                                              16    5631
NP_110415                                                              16    5631
NP_789794                                                              16    5631
NP_001244246                                                           16    5631
NP_001136018                                                           16    5631
NP_004045                                                              16    5631
NP_361013                                                              16    5631
XP_006722966                                                           16    5631
XP_006722965                                                           16    5631
XP_006722964                                                           16    5631
XP_005251132                                                           16    5631
XP_005263163                                                           16    5631
NP_001129074                                                           16    7355
NP_001073279                                                           16    7355
NP_000395                                                              16    7355
NP_001185739                                                           16    7355
NP_003618                                                              16    7355
XP_005259159                                                           16    7355
XP_005274415                                                           16    7355
XP_005274414                                                           16    7355
NP_065913                                                              16    7355
NP_787050                                                              15    9605
NP_001273171                                                           15    9605
NP_001511                                                              15    9605
NP_001139659                                                           15    9605
NP_001139658                                                           15    9605
NP_001139656                                                           15    9605
NP_006201                                                              15    9605
NP_001139657                                                           15    9605
NP_056381                                                              15    9605
XP_006721105                                                           15    9605
XP_006719863                                                           15    9605
XP_005266390                                                           15    9605
XP_006719862                                                           15    9605
XP_005266389                                                           15    9605
XP_005248537                                                           15    9605
XP_005248536                                                           15    9605
>NP_115927
          Length = 1321

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTDAFLSTS 9
           GTDA LSTS
Sbjct: 314 GTDALLSTS 322
>XP_006715292
          Length = 759

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTDAFLSTS 9
           GTDA LSTS
Sbjct: 301 GTDALLSTS 309
>NP_055616
          Length = 2137

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           GTD FLS S
Sbjct: 536 GTDVFLSKS 544
>XP_006711879
          Length = 1364

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           GTD FLS S
Sbjct: 536 GTDVFLSKS 544
>XP_005273329
          Length = 1934

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           GTD FLS S
Sbjct: 333 GTDVFLSKS 341
>NP_060660
          Length = 672

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G+D FLS S
Sbjct: 118 GSDLFLSAS 126
>NP_110415
          Length = 470

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GTDAFLS 7
          GTD FLS
Sbjct: 7  GTDIFLS 13
>NP_789794
          Length = 715

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G+D FLS S
Sbjct: 118 GSDLFLSAS 126
>NP_001244246
          Length = 283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GTDAFLS 7
          GTD FLS
Sbjct: 7  GTDIFLS 13
>NP_001136018
          Length = 1076

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTDAFLST 8
           G DAFLS+
Sbjct: 360 GEDAFLSS 367
>NP_004045
          Length = 482

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TDAFLST 8
           +DAFLST
Sbjct: 286 SDAFLST 292
>NP_361013
          Length = 1076

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTDAFLST 8
           G DAFLS+
Sbjct: 360 GEDAFLSS 367
>XP_006722966
          Length = 492

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTDAFLST 8
           G +AFLST
Sbjct: 427 GPEAFLST 434
>XP_006722965
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTDAFLST 8
           G +AFLST
Sbjct: 471 GPEAFLST 478
>XP_006722964
          Length = 581

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTDAFLST 8
           G +AFLST
Sbjct: 516 GPEAFLST 523
>XP_005251132
          Length = 470

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GTDAFLS 7
          GTD FLS
Sbjct: 7  GTDIFLS 13
>XP_005263163
          Length = 716

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G+D FLS S
Sbjct: 118 GSDLFLSAS 126
>NP_001129074
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TDAFLS 7
           TDAFLS
Sbjct: 118 TDAFLS 123
>NP_001073279
          Length = 647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TDAFLS 7
           TDAFLS
Sbjct: 219 TDAFLS 224
>NP_000395
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TDAFLS 7
           TDAFLS
Sbjct: 249 TDAFLS 254
>NP_001185739
          Length = 559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTDAFLS 7
           G+DAF+S
Sbjct: 271 GSDAFMS 277
>NP_003618
          Length = 559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTDAFLS 7
           G+DAF+S
Sbjct: 271 GSDAFMS 277
>XP_005259159
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   DAFLST 8
           DAFLST
Sbjct: 158 DAFLST 163
>XP_005274415
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTDAFLS 7
           G+DAF+S
Sbjct: 287 GSDAFMS 293
>XP_005274414
          Length = 585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTDAFLS 7
           G+DAF+S
Sbjct: 297 GSDAFMS 303
>NP_065913
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   DAFLST 8
           DAFLST
Sbjct: 158 DAFLST 163
>NP_787050
          Length = 887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDAFLSTS 9
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>NP_001273171
          Length = 2084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GTDAFLSTS 9
           G D FLS S
Sbjct: 470 GEDTFLSES 478
>NP_001511
          Length = 2109

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GTDAFLSTS 9
           G D FLS S
Sbjct: 470 GEDTFLSES 478
>NP_001139659
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G DAF+ +S
Sbjct: 566 GRDAFMQSS 574
>NP_001139658
          Length = 1588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G DAF+ +S
Sbjct: 690 GRDAFMQSS 698
>NP_001139656
          Length = 1588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G DAF+ +S
Sbjct: 690 GRDAFMQSS 698
>NP_006201
          Length = 1588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G DAF+ +S
Sbjct: 690 GRDAFMQSS 698
>NP_001139657
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GTDAFLSTS 9
           G DAF+ +S
Sbjct: 564 GRDAFMQSS 572
>NP_056381
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   DAFLSTS 9
           DAF STS
Sbjct: 310 DAFASTS 316
>XP_006721105
          Length = 2046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GTDAFLSTS 9
           G D FLS S
Sbjct: 470 GEDTFLSES 478
>XP_006719863
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDAFLSTS 9
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_005266390
          Length = 833

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDAFLSTS 9
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_006719862
          Length = 848

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDAFLSTS 9
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_005266389
          Length = 886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDAFLSTS 9
           TD++ STS
Sbjct: 158 TDSYFSTS 165
>XP_005248537
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   DAFLSTS 9
           DAF STS
Sbjct: 310 DAFASTS 316
>XP_005248536
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   DAFLSTS 9
           DAF STS
Sbjct: 372 DAFASTS 378
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.120    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 482,748
Number of extensions: 913
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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