Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              18    1135
NP_001231892                                                           16    7355
NP_003090                                                              16    7355
NP_001229862                                                           16    7355
NP_683758                                                              16    7355
NP_001104488                                                           16    7355
NP_660093                                                              16    7355
NP_079199                                                              16    7355
NP_892009                                                              16    7355
XP_005254668                                                           16    7355
XP_005254667                                                           16    7355
XP_006718429                                                           16    7355
XP_006718428                                                           16    7355
XP_006718427                                                           16    7355
NP_056321                                                              15    9605
NP_006258                                                              15    9605
NP_002200                                                              15    9605
NP_001107852                                                           15    9605
NP_001177738                                                           15    9605
NP_001253981                                                           15    9605
XP_005257983                                                           15    9605
XP_006721107                                                           15    9605
XP_005255370                                                           15    9605
XP_005264063                                                           15    9605
XP_005264062                                                           15    9605
XP_005264061                                                           15    9605
XP_005264060                                                           15    9605
XP_005264059                                                           15    9605
XP_005270781                                                           15    9605
NP_068750                                                              15    9605
NP_775259                                                              15    9605
NP_001028748                                                           15    9605
>NP_002430
          Length = 1137

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GSQTISGIV 9
           GSQ ISGIV
Sbjct: 485 GSQIISGIV 493
>NP_001231892
          Length = 2488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           SQT+SG V
Sbjct: 658 SQTVSGSV 665
>NP_003090
          Length = 522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GSQTISG 7
           G+QT+SG
Sbjct: 275 GTQTLSG 281
>NP_001229862
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GSQTISG 7
           G+QT+SG
Sbjct: 275 GTQTLSG 281
>NP_683758
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GSQTISG 7
           G+QT+SG
Sbjct: 210 GTQTLSG 216
>NP_001104488
          Length = 2365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           SQT+SG V
Sbjct: 571 SQTVSGSV 578
>NP_660093
          Length = 2429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           SQT+SG V
Sbjct: 635 SQTVSGSV 642
>NP_079199
          Length = 1887

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GSQTISGIV 9
            G+ T+SG+V
Sbjct: 1134 GNGTVSGLV 1142
>NP_892009
          Length = 2432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           SQT+SG V
Sbjct: 635 SQTVSGSV 642
>XP_005254668
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GSQTISG 7
           G+QT+SG
Sbjct: 117 GTQTLSG 123
>XP_005254667
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GSQTISG 7
           G+QT+SG
Sbjct: 177 GTQTLSG 183
>XP_006718429
          Length = 2416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           SQT+SG V
Sbjct: 586 SQTVSGSV 593
>XP_006718428
          Length = 2455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           SQT+SG V
Sbjct: 658 SQTVSGSV 665
>XP_006718427
          Length = 2465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           SQT+SG V
Sbjct: 635 SQTVSGSV 642
>NP_056321
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GSQTISGI 8
           G+Q ISG+
Sbjct: 141 GNQRISGV 148
>NP_006258
          Length = 3224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GSQTISGI 8
            GS+ +SGI
Sbjct: 1102 GSKNVSGI 1109
>NP_002200
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GSQTISGI 8
           G+Q +SGI
Sbjct: 571 GTQVLSGI 578
>NP_001107852
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GSQTISGI 8
           G+Q +SGI
Sbjct: 488 GTQVLSGI 495
>NP_001177738
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           S TISGIV
Sbjct: 317 SITISGIV 324
>NP_001253981
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QTISGIV 9
           QT+SGI+
Sbjct: 400 QTVSGIL 406
>XP_005257983
          Length = 850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QTISGIV 9
           QT+SGI+
Sbjct: 400 QTVSGIL 406
>XP_006721107
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GSQTISGI 8
           G+Q +SGI
Sbjct: 488 GTQVLSGI 495
>XP_005255370
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GSQTISGI 8
           G+Q +SGI
Sbjct: 571 GTQVLSGI 578
>XP_005264063
          Length = 3136

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GSQTISGI 8
            GS+ +SGI
Sbjct: 1102 GSKNVSGI 1109
>XP_005264062
          Length = 3232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GSQTISGI 8
            GS+ +SGI
Sbjct: 1076 GSKNVSGI 1083
>XP_005264061
          Length = 3232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GSQTISGI 8
            GS+ +SGI
Sbjct: 1102 GSKNVSGI 1109
>XP_005264060
          Length = 3250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GSQTISGI 8
            GS+ +SGI
Sbjct: 1102 GSKNVSGI 1109
>XP_005264059
          Length = 3258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GSQTISGI 8
            GS+ +SGI
Sbjct: 1102 GSKNVSGI 1109
>XP_005270781
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  GSQTISGI 8
          G+Q ISG+
Sbjct: 76 GNQRISGV 83
>NP_068750
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SQTISGIV 9
           S TISGIV
Sbjct: 348 SITISGIV 355
>NP_775259
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QTISGIV 9
           QT+SGI+
Sbjct: 400 QTVSGIL 406
>NP_001028748
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QTISGIV 9
           QT+SGI+
Sbjct: 400 QTVSGIL 406
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.130    0.330 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 457,366
Number of extensions: 800
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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