Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              21     229
NP_001075109                                                           21     229
XP_005263207                                                           17    2528
NP_065775                                                              17    2528
NP_112571                                                              17    3301
NP_001182317                                                           17    3301
NP_062550                                                              16    5631
XP_006726449                                                           16    5631
XP_006712222                                                           16    5631
NP_001230013                                                           16    7355
NP_851397                                                              16    7355
NP_001521                                                              16    7355
NP_079446                                                              16    7355
NP_079146                                                              16    7355
NP_057355                                                              16    7355
NP_001178253                                                           16    7355
XP_005258342                                                           16    7355
NP_001167558                                                           15    9605
NP_858045                                                              15    9605
NP_006525                                                              15    9605
NP_001020242                                                           15    9605
NP_001020241                                                           15    9605
NP_005608                                                              15    9605
NP_001519                                                              15    9605
NP_001129689                                                           15    9605
NP_055589                                                              15    9605
NP_060492                                                              15    9605
NP_775871                                                              15    9605
NP_004834                                                              15    9605
XP_006714853                                                           15    9605
XP_005248023                                                           15    9605
XP_006710785                                                           15    9605
XP_005245976                                                           15    9605
XP_005276064                                                           15    9605
NP_001129688                                                           15    9605
NP_001129690                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GSNRTETVT 9
            G+NRTETVT
Sbjct: 3861 GANRTETVT 3869
>NP_001075109
          Length = 4097

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GSNRTETVT 9
            G+NRTETVT
Sbjct: 3830 GANRTETVT 3838
>XP_005263207
          Length = 460

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GSNRTETVT 9
           GSN  ET+T
Sbjct: 149 GSNTNETIT 157
>NP_065775
          Length = 438

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GSNRTETVT 9
           GSN  ET+T
Sbjct: 149 GSNTNETIT 157
>NP_112571
          Length = 977

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GSNRTETVT 9
           G+ R+ETVT
Sbjct: 826 GAQRSETVT 834
>NP_001182317
          Length = 998

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GSNRTETVT 9
           G+ R+ETVT
Sbjct: 826 GAQRSETVT 834
>NP_062550
          Length = 393

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SNRTETVT 9
          +N TET+T
Sbjct: 20 TNHTETIT 27
>XP_006726449
          Length = 799

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           GSN TET
Sbjct: 375 GSNSTET 381
>XP_006712222
          Length = 799

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           GSN TET
Sbjct: 375 GSNSTET 381
>NP_001230013
          Length = 850

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           GSN TET
Sbjct: 438 GSNDTET 444
>NP_851397
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           GSN TET
Sbjct: 414 GSNDTET 420
>NP_001521
          Length = 826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           GSN TET
Sbjct: 414 GSNDTET 420
>NP_079446
          Length = 979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GSNRTETVT 9
           GSN T+T T
Sbjct: 236 GSNITDTCT 244
>NP_079146
          Length = 1606

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GSNRTETVT 9
           GSN T+T T
Sbjct: 236 GSNITDTCT 244
>NP_057355
          Length = 245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SNRTETVT 9
           SNR+ET T
Sbjct: 131 SNRSETST 138
>NP_001178253
          Length = 245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SNRTETVT 9
           SNR+ET T
Sbjct: 131 SNRSETST 138
>XP_005258342
          Length = 199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SNRTETVT 9
          SNR+ET T
Sbjct: 85 SNRSETST 92
>NP_001167558
          Length = 1423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GSNRTETVT 9
           G NR  TVT
Sbjct: 904 GPNRNVTVT 912
>NP_858045
          Length = 1424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GSNRTETVT 9
           G NR  TVT
Sbjct: 904 GPNRNVTVT 912
>NP_006525
          Length = 1420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GSNRTETVT 9
           G NR  TVT
Sbjct: 904 GPNRNVTVT 912
>NP_001020242
          Length = 151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           G NRT+T
Sbjct: 101 GGNRTKT 107
>NP_001020241
          Length = 151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           G NRT+T
Sbjct: 101 GGNRTKT 107
>NP_005608
          Length = 151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           G NRT+T
Sbjct: 101 GGNRTKT 107
>NP_001519
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GSNRTET 7
          G NRTE+
Sbjct: 38 GGNRTES 44
>NP_001129689
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GSNRTE 6
          GSN+TE
Sbjct: 47 GSNKTE 52
>NP_055589
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SNRTETV 8
           +NRT+TV
Sbjct: 346 TNRTDTV 352
>NP_060492
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GSNRTE 6
          GSN+TE
Sbjct: 47 GSNKTE 52
>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GSNRTETVT 9
            GS++TE  T
Sbjct: 6421 GSSKTEATT 6429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GSNRTETVT 9
            GS++TE  T
Sbjct: 6781 GSSKTEATT 6789
>NP_004834
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SNRTETV 8
          SN+T+TV
Sbjct: 75 SNKTQTV 81
>XP_006714853
          Length = 178

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           G NRT+T
Sbjct: 101 GGNRTKT 107
>XP_005248023
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GSNRTET 7
          G NRTE+
Sbjct: 38 GGNRTES 44
>XP_006710785
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GSNRTE 6
          GSN+TE
Sbjct: 47 GSNKTE 52
>XP_005245976
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GSNRTE 6
          GSN+TE
Sbjct: 47 GSNKTE 52
>XP_005276064
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GSNRTET 7
           G NRT+T
Sbjct: 107 GGNRTKT 113
>NP_001129688
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GSNRTE 6
          GSN+TE
Sbjct: 47 GSNKTE 52
>NP_001129690
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GSNRTE 6
          GSN+TE
Sbjct: 47 GSNKTE 52
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.108    0.271 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 444,333
Number of extensions: 1066
Number of successful extensions: 37
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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