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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           21     229
NP_036462                                                              21     229
NP_001243397                                                           21     229
XP_005269721                                                           21     229
NP_000386                                                              18    1935
XP_005261397                                                           18    1935
NP_056161                                                              17    3301
NP_036221                                                              17    3301
XP_006720156                                                           17    3301
NP_001254513                                                           17    4312
NP_055763                                                              17    4312
NP_892003                                                              17    4312
NP_077719                                                              16    5631
NP_005535                                                              16    5631
NP_001120648                                                           16    5631
NP_114145                                                              16    5631
XP_005258423                                                           16    5631
XP_005258424                                                           16    5631
XP_005258422                                                           16    5631
XP_006712575                                                           16    5631
XP_005246591                                                           16    5631
XP_005270959                                                           16    5631
XP_005270958                                                           16    5631
XP_006710730                                                           16    5631
NP_003820                                                              15    9605
NP_001248335                                                           15    9605
NP_001248336                                                           15    9605
NP_055918                                                              15    9605
XP_005251680                                                           15    9605
XP_006716954                                                           15    9605
XP_006716953                                                           15    9605
XP_006716952                                                           15    9605
XP_006716951                                                           15    9605
XP_006716950                                                           15    9605
XP_006716949                                                           15    9605
XP_006716948                                                           15    9605
XP_005251679                                                           15    9605

>NP_001243398
          Length = 1781

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GSMSAFTGR 9
           GSM+AFTGR
Sbjct: 361 GSMNAFTGR 369



>NP_036462
          Length = 2073

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GSMSAFTGR 9
           GSM+AFTGR
Sbjct: 361 GSMNAFTGR 369



>NP_001243397
          Length = 1890

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GSMSAFTGR 9
           GSM+AFTGR
Sbjct: 361 GSMNAFTGR 369



>XP_005269721
          Length = 2073

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GSMSAFTGR 9
           GSM+AFTGR
Sbjct: 361 GSMNAFTGR 369



>NP_000386
          Length = 897

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GSMSAFT 7
           GSMSAFT
Sbjct: 498 GSMSAFT 504



>XP_005261397
          Length = 903

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GSMSAFT 7
           GSMSAFT
Sbjct: 504 GSMSAFT 510



>NP_056161
          Length = 2539

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GSMSAFTGR 9
           GS+SA +GR
Sbjct: 802 GSLSAMSGR 810



>NP_036221
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GSMSAFTG 8
          GS+S FTG
Sbjct: 38 GSLSPFTG 45



>XP_006720156
          Length = 2497

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GSMSAFTGR 9
           GS+SA +GR
Sbjct: 802 GSLSAMSGR 810



>NP_001254513
          Length = 512

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GSMSAFTGR 9
           GSM +FT R
Sbjct: 440 GSMPSFTAR 448



>NP_055763
          Length = 541

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GSMSAFTGR 9
           GSM +FT R
Sbjct: 440 GSMPSFTAR 448



>NP_892003
          Length = 467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GSMSAFTGR 9
           GS+S +TGR
Sbjct: 450 GSVSIWTGR 458



>NP_077719
          Length = 2471

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SAFTGR 9
            SAFTGR
Sbjct: 1294 SAFTGR 1299



>NP_005535
          Length = 1242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    GSMSAFT 7
            G MSAFT
Sbjct: 1067 GGMSAFT 1073



>NP_001120648
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GSMSAFTGR 9
           G ++AF GR
Sbjct: 171 GQLAAFAGR 179



>NP_114145
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GSMSAFTGR 9
           G ++AF GR
Sbjct: 157 GQLAAFAGR 165



>XP_005258423
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GSMSAFTGR 9
           G ++AF GR
Sbjct: 157 GQLAAFAGR 165



>XP_005258424
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GSMSAFTGR 9
           G ++AF GR
Sbjct: 157 GQLAAFAGR 165



>XP_005258422
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GSMSAFTGR 9
           G ++AF GR
Sbjct: 157 GQLAAFAGR 165



>XP_006712575
          Length = 1242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    GSMSAFT 7
            G MSAFT
Sbjct: 1067 GGMSAFT 1073



>XP_005246591
          Length = 1242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    GSMSAFT 7
            G MSAFT
Sbjct: 1067 GGMSAFT 1073



>XP_005270959
          Length = 2432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SAFTGR 9
            SAFTGR
Sbjct: 1255 SAFTGR 1260



>XP_005270958
          Length = 2432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SAFTGR 9
            SAFTGR
Sbjct: 1255 SAFTGR 1260



>XP_006710730
          Length = 2432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SAFTGR 9
            SAFTGR
Sbjct: 1255 SAFTGR 1260



>NP_003820
          Length = 2041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1797 GSLSSFT 1803



>NP_001248335
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1793 GSLSSFT 1799



>NP_001248336
          Length = 2008

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1764 GSLSSFT 1770



>NP_055918
          Length = 1925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GSMSAFTGR 9
            GS+ A TG+
Sbjct: 1528 GSIDAITGK 1536



>XP_005251680
          Length = 2000

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1756 GSLSSFT 1762



>XP_006716954
          Length = 2008

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1764 GSLSSFT 1770



>XP_006716953
          Length = 2031

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1787 GSLSSFT 1793



>XP_006716952
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1793 GSLSSFT 1799



>XP_006716951
          Length = 2041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1797 GSLSSFT 1803



>XP_006716950
          Length = 2070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1826 GSLSSFT 1832



>XP_006716949
          Length = 2070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1826 GSLSSFT 1832



>XP_006716948
          Length = 2070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1826 GSLSSFT 1832



>XP_005251679
          Length = 2070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GSMSAFT 7
            GS+S+FT
Sbjct: 1826 GSLSSFT 1832



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.126    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 416,657
Number of extensions: 699
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)