Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     509
NP_001074003                                                           17    3301
XP_005254452                                                           17    3301
XP_005254451                                                           17    3301
NP_001129663                                                           17    4312
NP_056308                                                              17    4312
NP_001092108                                                           17    4312
NP_031399                                                              16    5631
NP_001255968                                                           16    5631
XP_005255623                                                           16    5631
NP_076936                                                              16    5631
NP_008835                                                              16    7355
NP_001012270                                                           16    7355
NP_001075109                                                           16    7355
NP_004769                                                              16    7355
NP_001271308                                                           16    7355
NP_001271306                                                           16    7355
NP_065939                                                              16    7355
NP_001185901                                                           16    7355
XP_005254569                                                           16    7355
XP_006710855                                                           16    7355
XP_006710854                                                           16    7355
NP_000929                                                              16    7355
NP_000114                                                              15    9605
NP_057723                                                              15    9605
NP_000693                                                              15    9605
NP_036270                                                              15    9605
NP_001539                                                              15    9605
NP_001257856                                                           15    9605
NP_001257859                                                           15    9605
NP_001257857                                                           15    9605
NP_001257858                                                           15    9605
NP_659444                                                              15    9605
NP_036399                                                              15    9605
NP_001271236                                                           15    9605
NP_001271235                                                           15    9605
NP_115548                                                              15    9605
NP_071918                                                              15    9605
NP_001191354                                                           15    9605
NP_055646                                                              15    9605
XP_005258760                                                           15    9605
XP_005258758                                                           15    9605
XP_005258757                                                           15    9605
XP_005258690                                                           15    9605
XP_005258689                                                           15    9605
XP_005254649                                                           15    9605
XP_005254648                                                           15    9605
XP_005249939                                                           15    9605
XP_006715854                                                           15    9605
XP_006715853                                                           15    9605
XP_006713495                                                           15    9605
XP_006713156                                                           15    9605
XP_006713155                                                           15    9605
XP_006710884                                                           15    9605
NP_001123578                                                           15    9605
NP_997228                                                              15    9605
>NP_004295
          Length = 1620

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GSLRKLRRA 9
           GS+RKLRRA
Sbjct: 130 GSVRKLRRA 138
>NP_001074003
          Length = 2214

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GSLRKLRR 8
           G+LRKLR+
Sbjct: 179 GALRKLRK 186
>XP_005254452
          Length = 2188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GSLRKLRR 8
           G+LRKLR+
Sbjct: 179 GALRKLRK 186
>XP_005254451
          Length = 2214

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GSLRKLRR 8
           G+LRKLR+
Sbjct: 179 GALRKLRK 186
>NP_001129663
          Length = 851

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GSLRKLRR 8
           G+LRKLR+
Sbjct: 194 GALRKLRQ 201
>NP_056308
          Length = 859

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GSLRKLRR 8
           G+LRKLR+
Sbjct: 194 GALRKLRQ 201
>NP_001092108
          Length = 708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GSLRKLRR 8
           GSL+KLR+
Sbjct: 635 GSLKKLRQ 642
>NP_031399
          Length = 582

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GSLRKLR 7
           G+LRKLR
Sbjct: 445 GNLRKLR 451
>NP_001255968
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GSLRKLR 7
           G+LRKLR
Sbjct: 399 GNLRKLR 405
>XP_005255623
          Length = 585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SLRKLRRA 9
           S RKLRRA
Sbjct: 513 SSRKLRRA 520
>NP_076936
          Length = 585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SLRKLRRA 9
           S RKLRRA
Sbjct: 513 SSRKLRRA 520
>NP_008835
          Length = 4128

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GSLRKLRR 8
            GSL +LRR
Sbjct: 1705 GSLEELRR 1712
>NP_001012270
          Length = 137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  SLRKLRR 8
          +LRKLRR
Sbjct: 84 NLRKLRR 90
>NP_001075109
          Length = 4097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GSLRKLRR 8
            GSL +LRR
Sbjct: 1705 GSLEELRR 1712
>NP_004769
          Length = 395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LRKLRRA 9
           LRKLRR+
Sbjct: 312 LRKLRRS 318
>NP_001271308
          Length = 146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLRKLRRA 9
           SLRKL++A
Sbjct: 131 SLRKLKQA 138
>NP_001271306
          Length = 140

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLRKLRRA 9
           SLRKL++A
Sbjct: 125 SLRKLKQA 132
>NP_065939
          Length = 1094

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLRKLRR 8
           SLRKL+R
Sbjct: 490 SLRKLKR 496
>NP_001185901
          Length = 1035

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLRKLRR 8
           SLRKL+R
Sbjct: 431 SLRKLKR 437
>XP_005254569
          Length = 167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLRKLRRA 9
           SLRKL++A
Sbjct: 152 SLRKLKQA 159
>XP_006710855
          Length = 821

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLRKLRR 8
           SLRKL+R
Sbjct: 217 SLRKLKR 223
>XP_006710854
          Length = 1046

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLRKLRR 8
           SLRKL+R
Sbjct: 442 SLRKLKR 448
>NP_000929
          Length = 1174

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLRKLRR 8
           +LRKLRR
Sbjct: 586 TLRKLRR 592
>NP_000114
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    RKLRRA 9
            RKLRRA
Sbjct: 1174 RKLRRA 1179
>NP_057723
          Length = 129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GSLRKLRR 8
          GSLR+L R
Sbjct: 4  GSLRRLLR 11
>NP_000693
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GSLRKLR 7
           GS+RK+R
Sbjct: 463 GSVRKMR 469
>NP_036270
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GSLRKLRRA 9
          GS+RKL  A
Sbjct: 54 GSIRKLASA 62
>NP_001539
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LRKLRR 8
           LRKLRR
Sbjct: 427 LRKLRR 432
>NP_001257856
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LRKLRR 8
           LRKLRR
Sbjct: 427 LRKLRR 432
>NP_001257859
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LRKLRR 8
           LRKLRR
Sbjct: 396 LRKLRR 401
>NP_001257857
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LRKLRR 8
           LRKLRR
Sbjct: 396 LRKLRR 401
>NP_001257858
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LRKLRR 8
           LRKLRR
Sbjct: 396 LRKLRR 401
>NP_659444
          Length = 1033

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GSLRKL 6
           GSLRKL
Sbjct: 746 GSLRKL 751
>NP_036399
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           G+LRKL R
Sbjct: 187 GALRKLLR 194
>NP_001271236
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKLRRA 9
           S+RK RRA
Sbjct: 241 SIRKKRRA 248
>NP_001271235
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKLRRA 9
           S+RK RRA
Sbjct: 198 SIRKKRRA 205
>NP_115548
          Length = 1316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           GSLRK+ R
Sbjct: 943 GSLRKVDR 950
>NP_071918
          Length = 1883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SLRKLRRA 9
            S+RK RRA
Sbjct: 1013 SIRKKRRA 1020
>NP_001191354
          Length = 1640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    RKLRRA 9
            RKLRRA
Sbjct: 1628 RKLRRA 1633
>NP_055646
          Length = 2925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    RKLRRA 9
            RKLRRA
Sbjct: 2677 RKLRRA 2682
>XP_005258760
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           G+LRKL R
Sbjct: 233 GALRKLLR 240
>XP_005258758
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           G+LRKL R
Sbjct: 187 GALRKLLR 194
>XP_005258757
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           G+LRKL R
Sbjct: 187 GALRKLLR 194
>XP_005258690
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GSLRKL 6
           GSLRKL
Sbjct: 229 GSLRKL 234
>XP_005258689
          Length = 934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GSLRKL 6
           GSLRKL
Sbjct: 647 GSLRKL 652
>XP_005254649
          Length = 1819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SLRKLRRA 9
            S+RK RRA
Sbjct: 1036 SIRKKRRA 1043
>XP_005254648
          Length = 1906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SLRKLRRA 9
            S+RK RRA
Sbjct: 1036 SIRKKRRA 1043
>XP_005249939
          Length = 1324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           GSLRK+ R
Sbjct: 943 GSLRKVDR 950
>XP_006715854
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           GSLRK+ R
Sbjct: 943 GSLRKVDR 950
>XP_006715853
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           GSLRK+ R
Sbjct: 943 GSLRKVDR 950
>XP_006713495
          Length = 2969

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    RKLRRA 9
            RKLRRA
Sbjct: 2721 RKLRRA 2726
>XP_006713156
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           GSLR +RR
Sbjct: 444 GSLRVVRR 451
>XP_006713155
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           GSLR +RR
Sbjct: 444 GSLRVVRR 451
>XP_006710884
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GSLRKL 6
          GSLRKL
Sbjct: 81 GSLRKL 86
>NP_001123578
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSLRKLRR 8
           G+LRKL R
Sbjct: 187 GALRKLLR 194
>NP_997228
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GSLRKLR 7
           GSLRK++
Sbjct: 162 GSLRKIK 168
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.140    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 492,288
Number of extensions: 1028
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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