Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     869
XP_006716142                                                           19     869
XP_005250088                                                           19     869
XP_006716141                                                           19     869
XP_005250085                                                           19     869
XP_006716140                                                           19     869
XP_005250084                                                           19     869
XP_005250083                                                           19     869
XP_005250082                                                           19     869
NP_705838                                                              17    3301
NP_001164409                                                           17    4312
NP_057452                                                              17    4312
NP_001240318                                                           17    4312
XP_006722829                                                           17    4312
XP_005273475                                                           17    4312
NP_689744                                                              16    5631
NP_056140                                                              16    5631
NP_006505                                                              16    5631
XP_005269340                                                           16    5631
XP_005265428                                                           16    5631
NP_001034931                                                           16    5631
XP_006726824                                                           16    7355
XP_006722329                                                           16    7355
XP_006710096                                                           16    7355
NP_078858                                                              15    9605
NP_001278232                                                           15    9605
NP_001278214                                                           15    9605
NP_112200                                                              15    9605
NP_057110                                                              15    9605
NP_689472                                                              15    9605
NP_001239579                                                           15    9605
NP_079109                                                              15    9605
>NP_733751
          Length = 4911

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1580 GSGLGTFSA 1588
>XP_006716142
          Length = 4927

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1597 GSGLGTFSA 1605
>XP_005250088
          Length = 4928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1597 GSGLGTFSA 1605
>XP_006716141
          Length = 4959

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1597 GSGLGTFSA 1605
>XP_005250085
          Length = 4979

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1597 GSGLGTFSA 1605
>XP_006716140
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1597 GSGLGTFSA 1605
>XP_005250084
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1597 GSGLGTFSA 1605
>XP_005250083
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1596 GSGLGTFSA 1604
>XP_005250082
          Length = 4983

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GSGLRTFSA 9
            GSGL TFSA
Sbjct: 1597 GSGLGTFSA 1605
>NP_705838
          Length = 4069

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GSGLRTFS 8
            G GLR+FS
Sbjct: 3848 GEGLRSFS 3855
>NP_001164409
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSGLRTFS 8
           GSGL+T S
Sbjct: 267 GSGLKTMS 274
>NP_057452
          Length = 558

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSGLRTFS 8
           GSGL+T S
Sbjct: 321 GSGLKTMS 328
>NP_001240318
          Length = 430

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSGLRTFS 8
           GSGL+T S
Sbjct: 193 GSGLKTMS 200
>XP_006722829
          Length = 753

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSGLRTFS 8
           GSGL+T S
Sbjct: 516 GSGLKTMS 523
>XP_005273475
          Length = 635

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSGLRTFS 8
           GSG+ TFS
Sbjct: 613 GSGILTFS 620
>NP_689744
          Length = 393

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GSGLRTFSA 9
          GSG  TFS+
Sbjct: 42 GSGRETFSS 50
>NP_056140
          Length = 2266

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SGLRTFSA 9
           SGL TF+A
Sbjct: 440 SGLETFTA 447
>NP_006505
          Length = 1956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SGLRTF 7
           SGLRTF
Sbjct: 212 SGLRTF 217
>XP_005269340
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GSGLRTFSA 9
          GSG  TFS+
Sbjct: 42 GSGRETFSS 50
>XP_005265428
          Length = 1959

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SGLRTF 7
           SGLRTF
Sbjct: 215 SGLRTF 220
>NP_001034931
          Length = 147

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GSGLRTFSA 9
          GSG R FS+
Sbjct: 19 GSGARRFSS 27
>XP_006726824
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  GLRTFSA 9
          GLR FSA
Sbjct: 82 GLRAFSA 88
>XP_006722329
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  GLRTFSA 9
          GLR FSA
Sbjct: 82 GLRAFSA 88
>XP_006710096
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  GLRTFSA 9
          GLR FSA
Sbjct: 82 GLRAFSA 88
>NP_078858
          Length = 4981

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GSGLRTFS 8
            G GLRT S
Sbjct: 4679 GQGLRTSS 4686
>NP_001278232
          Length = 4983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GSGLRTFS 8
            G GLRT S
Sbjct: 4681 GQGLRTSS 4688
>NP_001278214
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GSGLRTFS 8
            G GLRT S
Sbjct: 4680 GQGLRTSS 4687
>NP_112200
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GSGLRTF 7
           G+GL TF
Sbjct: 128 GTGLHTF 134
>NP_057110
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GSGLRTFS 8
           GSG+ T+S
Sbjct: 222 GSGVTTYS 229
>NP_689472
          Length = 1910

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    GSGLRTFSA 9
            G GLRT  A
Sbjct: 1488 GEGLRTADA 1496
>NP_001239579
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GSGLRTFS 8
           GSG+ T+S
Sbjct: 152 GSGVTTYS 159
>NP_079109
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GSGLRTFSA 9
           G G R FSA
Sbjct: 244 GKGRRDFSA 252
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.131    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 571,806
Number of extensions: 968
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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