Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              17    2528
XP_006716142                                                           17    2528
XP_005250088                                                           17    2528
XP_006716141                                                           17    2528
XP_005250085                                                           17    2528
XP_006716140                                                           17    2528
XP_005250084                                                           17    2528
XP_005250083                                                           17    2528
XP_005250082                                                           17    2528
NP_055062                                                              16    5631
XP_006714485                                                           16    5631
XP_005263418                                                           16    5631
XP_005263417                                                           16    5631
XP_006714484                                                           16    5631
XP_005263416                                                           16    5631
XP_006714483                                                           16    5631
XP_005263415                                                           16    5631
NP_612395                                                              16    7355
NP_001122403                                                           16    7355
NP_004594                                                              16    7355
NP_001159375                                                           16    7355
XP_005250609                                                           16    7355
XP_006714477                                                           16    7355
NP_001167532                                                           15    9605
NP_057417                                                              15    9605
NP_963863                                                              15    9605
XP_005255284                                                           15    9605
XP_005255283                                                           15    9605
XP_006720936                                                           15    9605
XP_006720935                                                           15    9605
XP_005255282                                                           15    9605
XP_006715411                                                           15    9605
XP_006711741                                                           15    9605
XP_005245713                                                           15    9605
XP_005245712                                                           15    9605
XP_005245710                                                           15    9605
XP_005245711                                                           15    9605
XP_006711739                                                           15    9605
XP_005245709                                                           15    9605
XP_006711737                                                           15    9605
XP_005245706                                                           15    9605
XP_005245705                                                           15    9605
XP_005245704                                                           15    9605
NP_542781                                                              15    9605
NP_000979                                                              15    9605
NP_116193                                                              15    9605
>NP_733751
          Length = 4911

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 908 GRSKLKS 914
>XP_006716142
          Length = 4927

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 909 GRSKLKS 915
>XP_005250088
          Length = 4928

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 909 GRSKLKS 915
>XP_006716141
          Length = 4959

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 909 GRSKLKS 915
>XP_005250085
          Length = 4979

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 909 GRSKLKS 915
>XP_006716140
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 909 GRSKLKS 915
>XP_005250084
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 909 GRSKLKS 915
>XP_005250083
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 908 GRSKLKS 914
>XP_005250082
          Length = 4983

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   GRSKLKS 7
           GRSKLKS
Sbjct: 909 GRSKLKS 915
>NP_055062
          Length = 1499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 529 GRNKLKKIL 537
>XP_006714485
          Length = 1585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 574 GRNKLKKIL 582
>XP_005263418
          Length = 1654

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 684 GRNKLKKIL 692
>XP_005263417
          Length = 1660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 690 GRNKLKKIL 698
>XP_006714484
          Length = 1685

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 690 GRNKLKKIL 698
>XP_005263416
          Length = 1693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 690 GRNKLKKIL 698
>XP_006714483
          Length = 1695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 684 GRNKLKKIL 692
>XP_005263415
          Length = 1701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GR+KLK ++
Sbjct: 690 GRNKLKKIL 698
>NP_612395
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSKLKS 7
           GR KLKS
Sbjct: 333 GRKKLKS 339
>NP_001122403
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSKLKS 7
           GR KLKS
Sbjct: 333 GRKKLKS 339
>NP_004594
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RSKLKSL 8
          RSKLKS+
Sbjct: 90 RSKLKSI 96
>NP_001159375
          Length = 251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RSKLKSL 8
          RSKLKS+
Sbjct: 90 RSKLKSI 96
>XP_005250609
          Length = 280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RSKLKSL 8
          RSKLKS+
Sbjct: 90 RSKLKSI 96
>XP_006714477
          Length = 364

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSKLKS 7
           GR KLKS
Sbjct: 333 GRKKLKS 339
>NP_001167532
          Length = 1145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 918 GRSLFKSLL 926
>NP_057417
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GRS+ +SL+
Sbjct: 700 GRSRSRSLV 708
>NP_963863
          Length = 1178

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 914 GRSLFKSLL 922
>XP_005255284
          Length = 2581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GRS+ +SL+
Sbjct: 700 GRSRSRSLV 708
>XP_005255283
          Length = 2751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GRS+ +SL+
Sbjct: 700 GRSRSRSLV 708
>XP_006720936
          Length = 2751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GRS+ +SL+
Sbjct: 699 GRSRSRSLV 707
>XP_006720935
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GRS+ +SL+
Sbjct: 700 GRSRSRSLV 708
>XP_005255282
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSKLKSLI 9
           GRS+ +SL+
Sbjct: 700 GRSRSRSLV 708
>XP_006715411
          Length = 166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GRSKLKSL 8
          GRS+ KSL
Sbjct: 83 GRSRSKSL 90
>XP_006711741
          Length = 1141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 914 GRSLFKSLL 922
>XP_005245713
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 942 GRSLFKSLL 950
>XP_005245712
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 942 GRSLFKSLL 950
>XP_005245710
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 942 GRSLFKSLL 950
>XP_005245711
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 942 GRSLFKSLL 950
>XP_006711739
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 943 GRSLFKSLL 951
>XP_005245709
          Length = 1187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 960 GRSLFKSLL 968
>XP_006711737
          Length = 1193

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 966 GRSLFKSLL 974
>XP_005245706
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 989 GRSLFKSLL 997
>XP_005245705
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSKLKSLI 9
           GRS  KSL+
Sbjct: 989 GRSLFKSLL 997
>XP_005245704
          Length = 1239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GRSKLKSLI 9
            GRS  KSL+
Sbjct: 1012 GRSLFKSLL 1020
>NP_542781
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GRSKLKSL 8
           GRS+ KSL
Sbjct: 178 GRSRSKSL 185
>NP_000979
          Length = 136

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RSKLKSLI 9
          RSK+KS +
Sbjct: 65 RSKIKSFV 72
>NP_116193
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SKLKSLI 9
          SKLKSL+
Sbjct: 52 SKLKSLL 58
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.135    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 377,039
Number of extensions: 519
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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