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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     869
NP_444254                                                              19     869
NP_444253                                                              19     869
NP_444255                                                              19     869
XP_005247551                                                           19     869
XP_005247550                                                           19     869
XP_006713712                                                           19     869
XP_005247549                                                           19     869
NP_001182192                                                           17    4312
NP_004949                                                              15    9605
NP_009041                                                              15    9605
XP_005268084                                                           15    9605
XP_005263496                                                           15    9605
XP_005263495                                                           15    9605

>NP_444256
          Length = 1794

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1636 GRLSSMAMI 1644



>NP_444254
          Length = 1845

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1687 GRLSSMAMI 1695



>NP_444253
          Length = 1914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1756 GRLSSMAMI 1764



>NP_444255
          Length = 1863

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1705 GRLSSMAMI 1713



>XP_005247551
          Length = 1855

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1697 GRLSSMAMI 1705



>XP_005247550
          Length = 1856

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1698 GRLSSMAMI 1706



>XP_006713712
          Length = 1923

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1766 GRLSSMAMI 1774



>XP_005247549
          Length = 1924

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GRLSSMVMI 9
            GRLSSM MI
Sbjct: 1766 GRLSSMAMI 1774



>NP_001182192
          Length = 1033

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRLSSMVMI 9
           GR  SMVM+
Sbjct: 623 GRFDSMVML 631



>NP_004949
          Length = 2549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GRLSSM 6
           GRLSSM
Sbjct: 716 GRLSSM 721



>NP_009041
          Length = 2627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    GRLSSMV 7
            GRL SMV
Sbjct: 1860 GRLDSMV 1866



>XP_005268084
          Length = 2627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    GRLSSMV 7
            GRL SMV
Sbjct: 1860 GRLDSMV 1866



>XP_005263496
          Length = 2322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GRLSSM 6
           GRLSSM
Sbjct: 489 GRLSSM 494



>XP_005263495
          Length = 2549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GRLSSM 6
           GRLSSM
Sbjct: 716 GRLSSM 721



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.138    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 183,124
Number of extensions: 97
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)