Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           21     175
NP_055903                                                              21     175
NP_004477                                                              16    5631
XP_005251989                                                           16    5631
XP_005251988                                                           16    5631
XP_005251987                                                           16    5631
NP_619723                                                              16    7355
NP_115941                                                              16    7355
NP_001164015                                                           16    7355
NP_001273707                                                           16    7355
NP_113649                                                              16    7355
NP_001273706                                                           16    7355
NP_689733                                                              16    7355
NP_722576                                                              16    7355
NP_612642                                                              16    7355
NP_001164014                                                           16    7355
NP_064522                                                              16    7355
XP_005262424                                                           16    7355
XP_005272685                                                           16    7355
XP_005251782                                                           16    7355
XP_006717025                                                           16    7355
XP_006717024                                                           16    7355
XP_005251781                                                           16    7355
XP_005251780                                                           16    7355
XP_005251779                                                           16    7355
XP_005251778                                                           16    7355
XP_005251777                                                           16    7355
XP_005251776                                                           16    7355
XP_006716544                                                           16    7355
NP_596870                                                              15    9605
NP_060712                                                              15    9605
NP_055423                                                              15    9605
NP_001274739                                                           15    9605
NP_008877                                                              15    9605
NP_056453                                                              15    9605
NP_001032409                                                           15    9605
NP_955400                                                              15    9605
NP_001104595                                                           15    9605
XP_006726466                                                           15    9605
XP_006724647                                                           15    9605
XP_006724646                                                           15    9605
XP_006724645                                                           15    9605
XP_006724644                                                           15    9605
XP_006724642                                                           15    9605
XP_005272600                                                           15    9605
XP_005274250                                                           15    9605
XP_006718734                                                           15    9605
XP_006718733                                                           15    9605
XP_005274249                                                           15    9605
XP_006718732                                                           15    9605
XP_006718408                                                           15    9605
XP_006712969                                                           15    9605
XP_005275995                                                           15    9605
NP_001032410                                                           15    9605
NP_055191                                                              15    9605
>NP_001155973
          Length = 1833

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GREQAQIHS 9
           GREQAQIH+
Sbjct: 361 GREQAQIHN 369
>NP_055903
          Length = 1723

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GREQAQIHS 9
           GREQAQIH+
Sbjct: 361 GREQAQIHN 369
>NP_004477
          Length = 1002

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   REQAQIH 8
           REQ Q+H
Sbjct: 213 REQLQVH 219
>XP_005251989
          Length = 975

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   REQAQIH 8
           REQ Q+H
Sbjct: 186 REQLQVH 192
>XP_005251988
          Length = 1002

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   REQAQIH 8
           REQ Q+H
Sbjct: 213 REQLQVH 219
>XP_005251987
          Length = 1029

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   REQAQIH 8
           REQ Q+H
Sbjct: 240 REQLQVH 246
>NP_619723
          Length = 1065

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 490 GRELSSLHS 498
>NP_115941
          Length = 1132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 586 GRELSSLHS 594
>NP_001164015
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GREQAQI 7
           GREQA++
Sbjct: 124 GREQAEL 130
>NP_001273707
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          R+Q+Q+H
Sbjct: 73 RQQSQVH 79
>NP_113649
          Length = 500

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   REQAQIH 8
           R+Q+Q+H
Sbjct: 261 RQQSQVH 267
>NP_001273706
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          R+Q+Q+H
Sbjct: 75 RQQSQVH 81
>NP_689733
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  QAQIHS 9
          QAQ+HS
Sbjct: 48 QAQVHS 53
>NP_722576
          Length = 3080

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    REQAQIH 8
            REQ QIH
Sbjct: 2994 REQWQIH 3000
>NP_612642
          Length = 255

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GREQAQI 7
           GREQA++
Sbjct: 124 GREQAEL 130
>NP_001164014
          Length = 289

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GREQAQI 7
           GREQA++
Sbjct: 124 GREQAEL 130
>NP_064522
          Length = 841

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QAQIHS 9
           QAQ+HS
Sbjct: 512 QAQVHS 517
>XP_005262424
          Length = 2875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    REQAQIH 8
            REQ QIH
Sbjct: 2789 REQWQIH 2795
>XP_005272685
          Length = 815

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QAQIHS 9
           QAQ+HS
Sbjct: 512 QAQVHS 517
>XP_005251782
          Length = 1072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 497 GRELSSLHS 505
>XP_006717025
          Length = 1085

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 510 GRELSSLHS 518
>XP_006717024
          Length = 1086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 511 GRELSSLHS 519
>XP_005251781
          Length = 1128

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 553 GRELSSLHS 561
>XP_005251780
          Length = 1138

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 614 GRELSSLHS 622
>XP_005251779
          Length = 1160

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 614 GRELSSLHS 622
>XP_005251778
          Length = 1161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 586 GRELSSLHS 594
>XP_005251777
          Length = 1167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 614 GRELSSLHS 622
>XP_005251776
          Length = 1189

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GREQAQIHS 9
           GRE + +HS
Sbjct: 614 GRELSSLHS 622
>XP_006716544
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   REQAQIH 8
           R+Q+Q+H
Sbjct: 177 RQQSQVH 183
>NP_596870
          Length = 5604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    REQAQIH 8
            REQ +IH
Sbjct: 4638 REQQEIH 4644
>NP_060712
          Length = 570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   EQAQIHS 9
           +QAQ+H+
Sbjct: 467 QQAQVHA 473
>NP_055423
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EQAQIHS 9
          EQA +HS
Sbjct: 61 EQATVHS 67
>NP_001274739
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EQAQIHS 9
          EQA +HS
Sbjct: 61 EQATVHS 67
>NP_008877
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    QAQIHS 9
            QAQ+HS
Sbjct: 1403 QAQLHS 1408
>NP_056453
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   GREQAQIH 8
           GR++ Q+H
Sbjct: 401 GRQRNQVH 408
>NP_001032409
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EQAQIHS 9
          EQA +HS
Sbjct: 61 EQATVHS 67
>NP_955400
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   GREQAQIH 8
           GR++ Q+H
Sbjct: 393 GRQRNQVH 400
>NP_001104595
          Length = 1488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          RE++Q+H
Sbjct: 62 REESQLH 68
>XP_006726466
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GREQAQI 7
           GRE+AQ+
Sbjct: 307 GREKAQL 313
>XP_006724647
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          RE++Q+H
Sbjct: 62 REESQLH 68
>XP_006724646
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          RE++Q+H
Sbjct: 62 REESQLH 68
>XP_006724645
          Length = 1212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          RE++Q+H
Sbjct: 62 REESQLH 68
>XP_006724644
          Length = 1244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          RE++Q+H
Sbjct: 62 REESQLH 68
>XP_006724642
          Length = 1520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  REQAQIH 8
          RE++Q+H
Sbjct: 62 REESQLH 68
>XP_005272600
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EQAQIHS 9
          EQA +HS
Sbjct: 61 EQATVHS 67
>XP_005274250
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    QAQIHS 9
            QAQ+HS
Sbjct: 1403 QAQLHS 1408
>XP_006718734
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    QAQIHS 9
            QAQ+HS
Sbjct: 1403 QAQLHS 1408
>XP_006718733
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    QAQIHS 9
            QAQ+HS
Sbjct: 1403 QAQLHS 1408
>XP_005274249
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    QAQIHS 9
            QAQ+HS
Sbjct: 1403 QAQLHS 1408
>XP_006718732
          Length = 2397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    QAQIHS 9
            QAQ+HS
Sbjct: 1410 QAQLHS 1415
>XP_006718408
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   GREQAQIH 8
           G E +QIH
Sbjct: 338 GEEYSQIH 345
>XP_006712969
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GREQAQI 7
           GRE+AQ+
Sbjct: 307 GREKAQL 313
>XP_005275995
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GREQAQI 7
           GRE+AQ+
Sbjct: 307 GREKAQL 313
>NP_001032410
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EQAQIHS 9
          EQA +HS
Sbjct: 61 EQATVHS 67
>NP_055191
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EQAQIHS 9
          EQA +HS
Sbjct: 61 EQATVHS 67
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.121    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 636,068
Number of extensions: 1278
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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