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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              19     665
NP_009297                                                              19     665
NP_055603                                                              16    5631
NP_150088                                                              16    5631
NP_003413                                                              16    5631
NP_932172                                                              16    5631
XP_006723423                                                           16    5631
XP_006723422                                                           16    5631
XP_006723421                                                           16    5631
XP_005259261                                                           16    5631
XP_005259260                                                           16    5631
XP_006717402                                                           16    5631
XP_006717401                                                           16    5631
XP_005252380                                                           16    5631
XP_005252379                                                           16    5631
XP_005252378                                                           16    5631
XP_005252377                                                           16    5631
NP_115575                                                              16    7355
NP_569735                                                              16    7355
NP_065931                                                              16    7355
NP_077015                                                              16    7355
NP_001244899                                                           16    7355
NP_009041                                                              15    9605
NP_004590                                                              15    9605
NP_689997                                                              15    9605
XP_006724373                                                           15    9605
XP_005256177                                                           15    9605
XP_005268084                                                           15    9605
XP_005248339                                                           15    9605
NP_006741                                                              15    9605

>NP_005148
          Length = 1130

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GQRSILLRY 9
           GQRSI LRY
Sbjct: 159 GQRSISLRY 167



>NP_009297
          Length = 1149

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GQRSILLRY 9
           GQRSI LRY
Sbjct: 178 GQRSISLRY 186



>NP_055603
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 258 ERSLLLKY 265



>NP_150088
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 258 ERSLLLKY 265



>NP_003413
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           G+RS+LL++
Sbjct: 523 GRRSVLLQH 531



>NP_932172
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           G+RS+LL++
Sbjct: 523 GRRSVLLQH 531



>XP_006723423
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           G+RS+LL++
Sbjct: 523 GRRSVLLQH 531



>XP_006723422
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           G+RS+LL++
Sbjct: 523 GRRSVLLQH 531



>XP_006723421
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           G+RS+LL++
Sbjct: 508 GRRSVLLQH 516



>XP_005259261
          Length = 775

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           G+RS+LL++
Sbjct: 564 GRRSVLLQH 572



>XP_005259260
          Length = 775

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           G+RS+LL++
Sbjct: 564 GRRSVLLQH 572



>XP_006717402
          Length = 392

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 208 ERSLLLKY 215



>XP_006717401
          Length = 392

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 208 ERSLLLKY 215



>XP_005252380
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 258 ERSLLLKY 265



>XP_005252379
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 258 ERSLLLKY 265



>XP_005252378
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 258 ERSLLLKY 265



>XP_005252377
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRSILLRY 9
           +RS+LL+Y
Sbjct: 258 ERSLLLKY 265



>NP_115575
          Length = 1188

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQRSILLRY 9
           GQ+  LL+Y
Sbjct: 138 GQKEALLKY 146



>NP_569735
          Length = 376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GQRSILLRY 9
           GQR  LLR+
Sbjct: 132 GQRRTLLRH 140



>NP_065931
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   GQRSILLRY 9
           GQ+S+L+++
Sbjct: 417 GQKSVLIQH 425



>NP_077015
          Length = 1004

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRSILLR 8
           GQ+ +LLR
Sbjct: 144 GQKEVLLR 151



>NP_001244899
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRSILLR 8
           GQ+ +LLR
Sbjct: 144 GQKEVLLR 151



>NP_009041
          Length = 2627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRSILLR 8
           G+R +LLR
Sbjct: 912 GERDLLLR 919



>NP_004590
          Length = 1141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GQRSILL 7
            GQR++LL
Sbjct: 1100 GQRAVLL 1106



>NP_689997
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GQRSILL 7
           GQR ILL
Sbjct: 250 GQRGILL 256



>XP_006724373
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GQRSILL 7
            GQR++LL
Sbjct: 1070 GQRAVLL 1076



>XP_005256177
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GQRSILLRY 9
           G  SIL RY
Sbjct: 308 GSSSILYRY 316



>XP_005268084
          Length = 2627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRSILLR 8
           G+R +LLR
Sbjct: 912 GERDLLLR 919



>XP_005248339
          Length = 2584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SILLRY 9
          SILLRY
Sbjct: 12 SILLRY 17



>NP_006741
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GQRSILLRY 9
           G  SIL RY
Sbjct: 475 GSSSILYRY 483



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.151    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 422,196
Number of extensions: 869
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)