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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005912                                                              18    1135
XP_005248577                                                           18    1135
XP_005248576                                                           18    1135
NP_079358                                                              17    3301
XP_006721341                                                           17    3301
XP_006721340                                                           17    3301
NP_056029                                                              16    5631
NP_001157706                                                           16    5631
NP_001157704                                                           16    5631
NP_703143                                                              16    5631
XP_005273513                                                           16    5631
XP_005273511                                                           16    5631
XP_005273512                                                           16    5631
NP_000483                                                              16    7355
NP_055029                                                              16    7355
NP_001185863                                                           16    7355
NP_258261                                                              16    7355
XP_006715905                                                           16    7355
XP_005249527                                                           16    7355
XP_006715303                                                           16    7355
NP_001098985                                                           15    9605
NP_005836                                                              15    9605
NP_004906                                                              15    9605
NP_997511                                                              15    9605
NP_058198                                                              15    9605
NP_997512                                                              15    9605
NP_997510                                                              15    9605
NP_997057                                                              15    9605
NP_057290                                                              15    9605
NP_001171516                                                           15    9605
NP_001171515                                                           15    9605
NP_079533                                                              15    9605
XP_005254082                                                           15    9605
XP_006719977                                                           15    9605
XP_005264809                                                           15    9605
NP_001135977                                                           15    9605
NP_071452                                                              15    9605

>NP_005912
          Length = 1512

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQRLRIAVF 9
            GQRLRIA F
Sbjct: 1381 GQRLRIADF 1389



>XP_005248577
          Length = 1349

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQRLRIAVF 9
            GQRLRIA F
Sbjct: 1218 GQRLRIADF 1226



>XP_005248576
          Length = 1375

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQRLRIAVF 9
            GQRLRIA F
Sbjct: 1244 GQRLRIADF 1252



>NP_079358
          Length = 561

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   QRLRIAVF 9
           QRLR++VF
Sbjct: 155 QRLRLSVF 162



>XP_006721341
          Length = 458

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  QRLRIAVF 9
          QRLR++VF
Sbjct: 52 QRLRLSVF 59



>XP_006721340
          Length = 458

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  QRLRIAVF 9
          QRLR++VF
Sbjct: 52 QRLRLSVF 59



>NP_056029
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G+R+R AV+
Sbjct: 94  GERMRYAVY 102



>NP_001157706
          Length = 227

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G+R+R AV+
Sbjct: 94  GERMRYAVY 102



>NP_001157704
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G+R+R AV+
Sbjct: 94  GERMRYAVY 102



>NP_703143
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QRLRIAVF 9
           +RLR AVF
Sbjct: 165 ERLRFAVF 172



>XP_005273513
          Length = 681

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G+R+R AV+
Sbjct: 94  GERMRYAVY 102



>XP_005273511
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G+R+R AV+
Sbjct: 94  GERMRYAVY 102



>XP_005273512
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G+R+R AV+
Sbjct: 94  GERMRYAVY 102



>NP_000483
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G ++RIA+F
Sbjct: 149 GMQMRIAMF 157



>NP_055029
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GQRLRIA 7
           GQRLR++
Sbjct: 154 GQRLRVS 160



>NP_001185863
          Length = 1492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR RIA+
Sbjct: 729 GQRARIAL 736



>NP_258261
          Length = 1464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR RIA+
Sbjct: 701 GQRARIAL 708



>XP_006715905
          Length = 1588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQRLRIAVF 9
           G ++RIA+F
Sbjct: 257 GMQMRIAMF 265



>XP_005249527
          Length = 822

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR RIA+
Sbjct: 729 GQRARIAL 736



>XP_006715303
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR RIA+
Sbjct: 729 GQRARIAL 736



>NP_001098985
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           G RLR+A+
Sbjct: 163 GMRLRVAM 170



>NP_005836
          Length = 1325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           G RLR+A+
Sbjct: 163 GMRLRVAM 170



>NP_004906
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 220 GQRKRLAI 227



>NP_997511
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 198 GQRKRLAI 205



>NP_058198
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 220 GQRKRLAI 227



>NP_997512
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 217 GQRKRLAI 224



>NP_997510
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 222 GQRKRLAI 229



>NP_997057
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 231 GQRKRLAI 238



>NP_057290
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GQRLRIAVF 9
          GQ+ ++AVF
Sbjct: 40 GQQKKLAVF 48



>NP_001171516
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QRLRIAV 8
           QR+RIA+
Sbjct: 256 QRIRIAI 262



>NP_001171515
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QRLRIAV 8
           QR+RIA+
Sbjct: 290 QRIRIAI 296



>NP_079533
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QRLRIAV 8
           QR+RIA+
Sbjct: 332 QRIRIAI 338



>XP_005254082
          Length = 1282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           G RLR+A+
Sbjct: 120 GMRLRVAM 127



>XP_006719977
          Length = 1295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           G RLR+A+
Sbjct: 163 GMRLRVAM 170



>XP_005264809
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GQRLRIAVF 9
          GQ+ ++AVF
Sbjct: 40 GQQKKLAVF 48



>NP_001135977
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 204 GQRKRLAI 211



>NP_071452
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQRLRIAV 8
           GQR R+A+
Sbjct: 204 GQRKRLAI 211



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.158    0.447 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 409,494
Number of extensions: 594
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)