Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              21     175
NP_002778                                                              17    2528
NP_112503                                                              17    3301
NP_000094                                                              17    3301
XP_006720469                                                           17    3301
XP_005254249                                                           17    3301
XP_005254248                                                           17    3301
XP_005254247                                                           17    3301
NP_067009                                                              16    5631
NP_057062                                                              16    5631
NP_065147                                                              16    5631
NP_001106279                                                           16    5631
NP_001186256                                                           16    5631
NP_065390                                                              16    7355
NP_060580                                                              16    7355
NP_008922                                                              16    7355
NP_001020026                                                           16    7355
NP_689934                                                              16    7355
XP_005256100                                                           16    7355
XP_005256099                                                           16    7355
XP_005264840                                                           16    7355
NP_001035502                                                           15    9605
NP_001035501                                                           15    9605
NP_001073878                                                           15    9605
NP_976044                                                              15    9605
NP_001274320                                                           15    9605
NP_001035500                                                           15    9605
XP_005276413                                                           15    9605
XP_005252383                                                           15    9605
XP_005276087                                                           15    9605
>NP_115803
          Length = 1156

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GQMTRENIF 9
           GQMT+ENIF
Sbjct: 281 GQMTQENIF 289
>NP_002778
          Length = 234

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           GQMT +NI
Sbjct: 201 GQMTEDNI 208
>NP_112503
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           G +TREN+
Sbjct: 289 GDLTRENV 296
>NP_000094
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           G +TREN+
Sbjct: 289 GDLTRENV 296
>XP_006720469
          Length = 401

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           G +TREN+
Sbjct: 187 GDLTRENV 194
>XP_005254249
          Length = 444

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           G +TREN+
Sbjct: 230 GDLTRENV 237
>XP_005254248
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           G +TREN+
Sbjct: 289 GDLTRENV 296
>XP_005254247
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           G +TREN+
Sbjct: 289 GDLTRENV 296
>NP_067009
          Length = 540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQMTRENIF 9
           G +TR++IF
Sbjct: 271 GSVTRDSIF 279
>NP_057062
          Length = 835

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTRENIF 9
           +T+ENIF
Sbjct: 298 LTKENIF 304
>NP_065147
          Length = 859

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           G+MT EN+
Sbjct: 140 GEMTSENL 147
>NP_001106279
          Length = 949

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTRENIF 9
           +T+ENIF
Sbjct: 412 LTKENIF 418
>NP_001186256
          Length = 856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTRENIF 9
           +T+ENIF
Sbjct: 412 LTKENIF 418
>NP_065390
          Length = 317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTRENI 8
           GQ+T EN+
Sbjct: 270 GQLTLENL 277
>NP_060580
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QMTRENIF 9
           Q+T ENI+
Sbjct: 158 QITHENIY 165
>NP_008922
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MTRENI 8
         MTREN+
Sbjct: 1 MTRENV 6
>NP_001020026
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MTRENI 8
         MTREN+
Sbjct: 1 MTRENV 6
>NP_689934
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QMTRENIF 9
           Q+T ENI+
Sbjct: 158 QITHENIY 165
>XP_005256100
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QMTRENIF 9
           Q+T ENI+
Sbjct: 158 QITHENIY 165
>XP_005256099
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QMTRENIF 9
           Q+T ENI+
Sbjct: 158 QITHENIY 165
>XP_005264840
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MTRENI 8
         MTREN+
Sbjct: 1 MTRENV 6
>NP_001035502
          Length = 1031

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTRENIF 9
           G  +RENI+
Sbjct: 407 GMQSRENIW 415
>NP_001035501
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTRENIF 9
           G  +RENI+
Sbjct: 382 GMQSRENIW 390
>NP_001073878
          Length = 864

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRENIF 9
           TRENI+
Sbjct: 196 TRENIY 201
>NP_976044
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTRENIF 9
           G  +RENI+
Sbjct: 455 GMQSRENIW 463
>NP_001274320
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTRENIF 9
           G  +RENI+
Sbjct: 382 GMQSRENIW 390
>NP_001035500
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTRENIF 9
           G  +RENI+
Sbjct: 420 GMQSRENIW 428
>XP_005276413
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MTRENI 8
         MTREN+
Sbjct: 4 MTRENL 9
>XP_005252383
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MTRENI 8
         MTREN+
Sbjct: 4 MTRENL 9
>XP_005276087
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 MTRENI 8
         MTREN+
Sbjct: 4 MTRENL 9
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 428,346
Number of extensions: 567
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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