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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              22     134
NP_002778                                                              20     509
NP_001273708                                                           17    4312
NP_115669                                                              17    4312
XP_005252325                                                           17    4312
XP_006717365                                                           17    4312
XP_005252324                                                           17    4312
NP_002871                                                              16    5631
NP_067009                                                              16    5631
XP_005265417                                                           16    5631
XP_005265416                                                           16    5631
XP_005265415                                                           16    5631
XP_005265414                                                           16    5631
XP_005265412                                                           16    5631
NP_001618                                                              16    7355
NP_443161                                                              16    7355
NP_002301                                                              16    7355
NP_001121143                                                           16    7355
NP_001230212                                                           16    7355
NP_001230210                                                           16    7355
NP_001230209                                                           16    7355
XP_006710380                                                           16    7355
XP_005245788                                                           16    7355
XP_006710379                                                           16    7355
XP_006710378                                                           16    7355
NP_001020330                                                           15    9605
NP_055328                                                              15    9605
NP_001170936                                                           15    9605
NP_001020329                                                           15    9605
NP_004346                                                              15    9605
NP_078798                                                              15    9605
XP_006714874                                                           15    9605

>NP_115803
          Length = 1156

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GQMTQDNIF 9
           GQMTQ+NIF
Sbjct: 281 GQMTQENIF 289



>NP_002778
          Length = 234

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   GQMTQDNI 8
           GQMT+DNI
Sbjct: 201 GQMTEDNI 208



>NP_001273708
          Length = 991

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTQDNIF 9
           GQ+T D +F
Sbjct: 191 GQLTDDEMF 199



>NP_115669
          Length = 1013

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTQDNIF 9
           GQ+T D +F
Sbjct: 213 GQLTDDEMF 221



>XP_005252325
          Length = 1036

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTQDNIF 9
           GQ+T D +F
Sbjct: 236 GQLTDDEMF 244



>XP_006717365
          Length = 1062

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTQDNIF 9
           GQ+T D +F
Sbjct: 262 GQLTDDEMF 270



>XP_005252324
          Length = 1076

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQMTQDNIF 9
           GQ+T D +F
Sbjct: 276 GQLTDDEMF 284



>NP_002871
          Length = 648

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+DN+
Sbjct: 410 GYMTKDNL 417



>NP_067009
          Length = 540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQMTQDNIF 9
           G +T+D+IF
Sbjct: 271 GSVTRDSIF 279



>XP_005265417
          Length = 494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+DN+
Sbjct: 410 GYMTKDNL 417



>XP_005265416
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+DN+
Sbjct: 296 GYMTKDNL 303



>XP_005265415
          Length = 567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+DN+
Sbjct: 329 GYMTKDNL 336



>XP_005265414
          Length = 615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+DN+
Sbjct: 377 GYMTKDNL 384



>XP_005265412
          Length = 648

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+DN+
Sbjct: 410 GYMTKDNL 417



>NP_001618
          Length = 583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MTQDNIF 9
           +T+DN+F
Sbjct: 116 VTEDNVF 122



>NP_443161
          Length = 1412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QMTQDNI 8
           Q+T+DNI
Sbjct: 526 QVTEDNI 532



>NP_002301
          Length = 1097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TQDNIF 9
           T+DNIF
Sbjct: 318 TEDNIF 323



>NP_001121143
          Length = 1097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TQDNIF 9
           T+DNIF
Sbjct: 318 TEDNIF 323



>NP_001230212
          Length = 133

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MTQDNIF 9
           +T+DN+F
Sbjct: 116 VTEDNVF 122



>NP_001230210
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MTQDNIF 9
           +T+DN+F
Sbjct: 116 VTEDNVF 122



>NP_001230209
          Length = 570

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MTQDNIF 9
           +T+DN+F
Sbjct: 116 VTEDNVF 122



>XP_006710380
          Length = 1398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QMTQDNI 8
           Q+T+DNI
Sbjct: 526 QVTEDNI 532



>XP_005245788
          Length = 1434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QMTQDNI 8
           Q+T+DNI
Sbjct: 526 QVTEDNI 532



>XP_006710379
          Length = 1452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QMTQDNI 8
           Q+T+DNI
Sbjct: 526 QVTEDNI 532



>XP_006710378
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QMTQDNI 8
           Q+T+DNI
Sbjct: 526 QVTEDNI 532



>NP_001020330
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+D++
Sbjct: 135 GNMTEDHV 142



>NP_055328
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GQMTQD 6
          G+MTQD
Sbjct: 88 GRMTQD 93



>NP_001170936
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GQMTQD 6
           GQ+TQD
Sbjct: 989 GQVTQD 994



>NP_001020329
          Length = 160

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+D++
Sbjct: 135 GNMTEDHV 142



>NP_004346
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+D++
Sbjct: 135 GNMTEDHV 142



>NP_078798
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GQMTQDNIF 9
          GQ++QD  F
Sbjct: 87 GQLSQDRDF 95



>XP_006714874
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   GQMTQDNI 8
           G MT+D++
Sbjct: 212 GNMTEDHV 219



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.136    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 481,957
Number of extensions: 698
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)