Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results 
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done




                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     390
NP_671714                                                              20     390
XP_006715890                                                           20     390
XP_005250160                                                           20     390
XP_005250159                                                           20     390
NP_001034463                                                           18    1935
NP_976035                                                              16    5631
XP_005249548                                                           16    5631
NP_002496                                                              16    7355
NP_056083                                                              16    7355
XP_005247302                                                           16    7355
NP_005459                                                              16    7355
NP_005054                                                              15    9605
NP_075050                                                              15    9605
NP_001137295                                                           15    9605
NP_001120925                                                           15    9605
NP_004394                                                              15    9605
NP_001230633                                                           15    9605
NP_003400                                                              15    9605
NP_001230631                                                           15    9605
XP_005265644                                                           15    9605
XP_006713482                                                           15    9605



>NP_005742
          Length = 3907

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   QKIETMQF 9
           QKIETMQF
Sbjct: 676 QKIETMQF 683





>NP_671714
          Length = 3899

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   QKIETMQF 9
           QKIETMQF
Sbjct: 676 QKIETMQF 683





>XP_006715890
          Length = 3860

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   QKIETMQF 9
           QKIETMQF
Sbjct: 676 QKIETMQF 683





>XP_005250160
          Length = 3939

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   QKIETMQF 9
           QKIETMQF
Sbjct: 676 QKIETMQF 683





>XP_005250159
          Length = 3952

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   QKIETMQF 9
           QKIETMQF
Sbjct: 676 QKIETMQF 683





>NP_001034463
          Length = 534

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GQKIETMQ 8
           GQK+E+MQ
Sbjct: 118 GQKLESMQ 125





>NP_976035
          Length = 346

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQKIETMQF 9
           G +I+T+QF
Sbjct: 132 GTEIDTLQF 140





>XP_005249548
          Length = 342

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GQKIETMQF 9
           G +I+T+QF
Sbjct: 132 GTEIDTLQF 140





>NP_002496
          Length = 347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  GQKIETMQ 8
          GQ+++T+Q
Sbjct: 46 GQQVQTLQ 53





>NP_056083
          Length = 2243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GQKIETMQF 9
          G+K ET++F
Sbjct: 77 GKKSETLKF 85





>XP_005247302
          Length = 2248

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GQKIETMQF 9
          G+K ET++F
Sbjct: 77 GKKSETLKF 85





>NP_005459
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQKIETMQ 8
           GQ+I T+Q
Sbjct: 636 GQRISTLQ 643





>NP_005054
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GQKIETM 7
          G K+ETM
Sbjct: 32 GDKLETM 38





>NP_075050
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQKIETMQ 8
           GQK+ T+Q
Sbjct: 402 GQKMTTLQ 409





>NP_001137295
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   GQKIETMQ 8
           GQ++E+M+
Sbjct: 217 GQEVESME 224





>NP_001120925
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QKIETMQ 8
           QKI TMQ
Sbjct: 160 QKITTMQ 166





>NP_004394
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QKIETMQ 8
           QKI TMQ
Sbjct: 160 QKITTMQ 166





>NP_001230633
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   GQKIETMQ 8
           GQ++E+M+
Sbjct: 217 GQEVESME 224





>NP_003400
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   GQKIETMQ 8
           GQ++E+M+
Sbjct: 217 GQEVESME 224





>NP_001230631
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   GQKIETMQ 8
           GQ++E+M+
Sbjct: 217 GQEVESME 224





>XP_005265644
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQKIETMQ 8
           GQK+ T+Q
Sbjct: 402 GQKMTTLQ 409





>XP_006713482
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GQKIETMQ 8
           GQK+ T+Q
Sbjct: 402 GQKMTTLQ 409





  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.133    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 417,794
Number of extensions: 769
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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