Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
NP_653080                                                              19     665
XP_005267292                                                           19     665
XP_005267291                                                           19     665
XP_005267290                                                           19     665
NP_036223                                                              17    4312
NP_001131140                                                           16    7355
NP_036247                                                              16    7355
NP_001131139                                                           16    7355
NP_001278383                                                           15    9605
NP_055676                                                              15    9605
NP_001273456                                                           15    9605
NP_060530                                                              15    9605
NP_112599                                                              15    9605
NP_113609                                                              15    9605
XP_006722192                                                           15    9605
XP_006721418                                                           15    9605
>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQARSDISK 9
            GQA SDISK
Sbjct: 1624 GQAHSDISK 1632
>NP_653080
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQARSDISK 9
            GQA SDISK
Sbjct: 1526 GQAHSDISK 1534
>XP_005267292
          Length = 1677

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQARSDISK 9
            GQA SDISK
Sbjct: 1519 GQAHSDISK 1527
>XP_005267291
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQARSDISK 9
            GQA SDISK
Sbjct: 1526 GQAHSDISK 1534
>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GQARSDISK 9
            GQA SDISK
Sbjct: 1624 GQAHSDISK 1632
>NP_036223
          Length = 502

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QARSDISK 9
           QARS ISK
Sbjct: 428 QARSQISK 435
>NP_001131140
          Length = 1982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QARSDISK 9
           +A+SD+SK
Sbjct: 180 RAKSDVSK 187
>NP_036247
          Length = 1982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QARSDISK 9
           +A+SD+SK
Sbjct: 180 RAKSDVSK 187
>NP_001131139
          Length = 1982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QARSDISK 9
           +A+SD+SK
Sbjct: 180 RAKSDVSK 187
>NP_001278383
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQARSDISK 9
           GQ  +D+SK
Sbjct: 541 GQTGTDVSK 549
>NP_055676
          Length = 946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQARSDISK 9
           GQ  +D+SK
Sbjct: 692 GQTGTDVSK 700
>NP_001273456
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQARSDISK 9
           GQ  +D+SK
Sbjct: 692 GQTGTDVSK 700
>NP_060530
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ARSDISK 9
           AR DISK
Sbjct: 719 ARDDISK 725
>NP_112599
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  GQARSDI 7
          G+ARSD+
Sbjct: 25 GRARSDL 31
>NP_113609
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GQARSDI 7
           G+ARSD+
Sbjct: 572 GKARSDL 578
>XP_006722192
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GQARSDI 7
           G+ARSD+
Sbjct: 409 GKARSDL 415
>XP_006721418
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GQARSDISK 9
           GQ  +D+SK
Sbjct: 689 GQTGTDVSK 697
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.118    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 381,988
Number of extensions: 493
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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